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201.
Background
Using sequence-structure threading we have conducted structural characterization of complete proteomes of 37 archaeal, bacterial and eukaryotic organisms (including worm, fly, mouse and human) totaling 167,888 genes. 相似文献202.
Kim AT Verheijden Linette EM Willemsen Saskia Braber Thea Leusink-Muis Dianne JM Delsing Johan Garssen Aletta D Kraneveld Gert Folkerts 《Respiratory research》2015,16(1)
Background
Allergic asthma is strongly associated with the exposure to house dust mite (HDM) and is characterized by eosinophilic pulmonary inflammation and airway hyperresponsiveness (AHR). Recently, there is an increased interest in using dietary oligosaccharides, also known as prebiotics, as a novel strategy to prevent the development of, or reduce, symptoms of allergy.Aim
We investigated the preventive capacity of dietary galacto-oligosaccharides (GOS) compared to an intra-airway therapeutic treatment with budesonide on the development of HDM-induced allergic asthma in mice.Methods
BALB/c mice were intranasally sensitized with 1 μg HDM on day 0 followed by daily intranasal challenge with PBS or 10 μg HDM on days 7 to 11. Two weeks prior to the first sensitization and throughout the experiment mice were fed a control diet or a diet containing 1% GOS. Reference mice were oropharyngeally instilled with budesonide (500 μg/kg) on days 7, 9, 11, and 13, while being fed the control diet. On day 14, AHR was measured by nebulizing increasing doses of methacholine into the airways. At the end of the experiment, bronchoalveolar lavage fluid (BALF) and lungs were collected.Results
Sensitization and challenge with HDM resulted in AHR. In contrast to budesonide, dietary intervention with 1% GOS prevented the development of AHR. HDM sensitization and challenge resulted in a significant increase in BALF leukocytes numbers, which was suppressed by budesonide treatment and dietary intervention with 1% GOS. Moreover, HDM sensitization and challenge resulted in significantly enhanced concentrations of IL-6, CCL17, IL-33, CCL5 and IL-13 in lung tissue. Both dietary intervention with 1% GOS or budesonide treatment significantly decreased the HDM-induced increased concentrations of CCL5 and IL-13 in lung tissue, while budesonide also reduced the HDM-enhanced concentrations of IL-6 and CCL17 in lung tissue.Conclusion
Not only did dietary intervention with 1% GOS during sensitization and challenge prevent the induction of airway eosinophilia and Th2-related cytokine and chemokine concentrations in the lung equally effective as budesonide treatment, it also prevented AHR development in HDM-allergic mice. GOS might be useful for the prevention and/or treatment of symptoms in asthmatic disease.Electronic supplementary material
The online version of this article (doi:10.1186/s12931-015-0171-0) contains supplementary material, which is available to authorized users. 相似文献203.
Rob Jelier Guido Jenster Lambert CJ Dorssers Bas J Wouters Peter JM Hendriksen Barend Mons Ruud Delwel Jan A Kors 《BMC bioinformatics》2007,8(1):14
Background
High-throughput experiments, such as with DNA microarrays, typically result in hundreds of genes potentially relevant to the process under study, rendering the interpretation of these experiments problematic. Here, we propose and evaluate an approach to find functional associations between large numbers of genes and other biomedical concepts from free-text literature. For each gene, a profile of related concepts is constructed that summarizes the context in which the gene is mentioned in literature. We assign a weight to each concept in the profile based on a likelihood ratio measure. Gene concept profiles can then be clustered to find related genes and other concepts. 相似文献204.
Background
The definition of human MHC class I haplotypes through association of HLA-A, HLA-Cw and HLA-B has been used to analyze ethnicity, population migrations and disease association.Results
Here, we present HLA-E allele haplotype association and population linkage disequilibrium (LD) analysis within the ~1.3 Mb bounded by HLA-B/Cw and HLA-A to increase the resolution of identified class I haplotypes. Through local breakdown of LD, we inferred ancestral recombination points both upstream and downstream of HLA-E contributing to alternative block structures within previously identified haplotypes. Through single nucleotide polymorphism (SNP) analysis of the MHC region, we also confirmed the essential genetic fixity, previously inferred by MHC allele analysis, of three conserved extended haplotypes (CEHs), and we demonstrated that commercially-available SNP analysis can be used in the MHC to help define CEHs and CEH fragments.Conclusion
We conclude that to generate high-resolution maps for relating MHC haplotypes to disease susceptibility, both SNP and MHC allele analysis must be conducted as complementary techniques. 相似文献205.
206.
Maged1, a new regulator of skeletal myogenic differentiation and muscle regeneration 总被引:1,自引:0,他引:1
Tuan HN Nguyen Mathieu JM Bertrand Christiane Sterpin Younes Achouri Olivier RY De Backer 《BMC cell biology》2010,11(1):57
Background
In normal adult skeletal muscle, cell turnover is very slow. However, after an acute lesion or in chronic pathological conditions, such as primary myopathies, muscle stem cells, called satellite cells, are induced to proliferate, then withdraw definitively from the cell cycle and fuse to reconstitute functional myofibers. 相似文献207.
208.
Evolution of SINE S1 retroposons in Cruciferae plant species 总被引:3,自引:0,他引:3
Lenoir A; Cournoyer B; Warwick S; Picard G; Deragon JM 《Molecular biology and evolution》1997,14(9):934-941
The S1 element is a plant short interspersed element (SINE) that was first
described and studied in Brassica napus. In this work, we investigated the
distribution and the molecular phylogeny of the S1 element within the
Cruciferae (= Brassicaceae). S1 elements were found to be widely
distributed within the Cruciferae, especially in species of the tribe
Brassiceae. The molecular phylogeny of S1 elements in eight Cruciferae
species (Brassica oleracea, Brassica rapa, Brassica napus, Brassica nigra,
Sinapis, arvensis, Sinapis pubescens, Coincya monensis, and Vella spinosa)
was inferred using 14-36 elements per species. Significant neighbor-joining
and maximum-parsimony phylogenetic clusters, supported by high bootstrap P
values and/or represented in 100% of the most-parsimonious trees, were
observed for each species. Most of these clusters probably correspond to
recent species-specific bursts of S1 amplification. Since these species
diverged recently, S1 amplification in Cruciferae plants is proposed to be
a highly dynamic process that could contribute to genome rearrangements and
eventually lead to reproductive isolation. S1 sequence analysis also
revealed putative gene conversion events that occurred between different S1
elements of a given species. These events suggest that gene conversion is a
minor but significant component of the molecular drive governing S1
concerted evolution.
相似文献
209.
210.