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41.
High congruency of QTL positions for heterosis of grain yield in three crosses of maize 总被引:1,自引:0,他引:1
Chris C. Schön Baldev S. Dhillon H. Friedrich Utz Albrecht E. Melchinger 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(2):321-332
The genetic basis of heterosis in maize has been investigated in a number of studies but results have not been conclusive.
Here, we compare quantitative trait loci (QTL) mapping results for grain yield, grain moisture, and plant height from three
populations derived from crosses of the heterotic pattern Iowa Stiff Stalk Synthetic × Lancaster Sure Crop, investigated with
the Design III, and analyzed with advanced statistical methods specifically developed to examine the genetic basis of mid-parent
heterosis (MPH). In two populations, QTL analyses were conducted with a joint fit of linear transformations Z
1 (trait mean across pairs of backcross progenies) and Z
2 (half the trait difference between pairs of backcross progenies) to estimate augmented additive and augmented dominance effects
of each QTL, as well as their ratio. QTL results for the third population were obtained from the literature. For Z
2 of grain yield, congruency of QTL positions was high across populations, and a large proportion of the genetic variance (~70%)
was accounted for by QTL. This was not the case for Z
1 or the other two traits. Further, almost all congruent grain yield QTL were located in the same or an adjacent bin encompassing
the centromere. We conclude that different alleles have been fixed in each heterotic pool, which in combination with allele(s)
from the opposite heterotic pool lead to high MPH for grain yield. Their positive interactions very likely form the base line
for the superior performance of the heterotic pattern under study. 相似文献
42.
43.
Quantitative trait locus mapping based on resampling in a vast maize testcross experiment and its relevance to quantitative genetics for complex traits 总被引:17,自引:0,他引:17
From simulation studies it is known that the allocation of experimental resources has a crucial effect on power of QTL detection as well as on accuracy and precision of QTL estimates. In this study, we used a very large experimental data set composed of 976 F(5) maize testcross progenies evaluated in 19 environments and cross-validation to assess the effect of sample size (N), number of test environments (E), and significance threshold on the number of detected QTL, the proportion of the genotypic variance explained by them, and the corresponding bias of estimates for grain yield, grain moisture, and plant height. In addition, we used computer simulations to compare the usefulness of two cross-validation schemes for obtaining unbiased estimates of QTL effects. The maximum, validated genotypic variance explained by QTL in this study was 52.3% for grain moisture despite the large number of detected QTL, thus confirming the infinitesimal model of quantitative genetics. In both simulated and experimental data, the effect of sample size on power of QTL detection as well as on accuracy and precision of QTL estimates was large. The number of detected QTL and the proportion of genotypic variance explained by QTL generally increased more with increasing N than with increasing E. The average bias of QTL estimates and its range were reduced by increasing N and E. Cross-validation performed well with respect to yielding asymptotically unbiased estimates of the genotypic variance explained by QTL. On the basis of our findings, recommendations for planning of QTL mapping experiments and allocation of experimental resources are given. 相似文献
44.
M. Vuylsteke R. Mank R. Antonise E. Bastiaans M. L. Senior C. W. Stuber A. E. Melchinger T. Lübberstedt X. C. Xia P. Stam M. Zabeau M. Kuiper 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(6):921-935
This study demonstrates the relative ease of generating high-density linkage maps using the AFLP® technology. Two high-density AFLP linkage maps of Zea mays L. were generated based on: (1) a B73 × Mo17 recombinant inbred population and (2) a D32 × D145 immortalized F2 population. Although AFLP technology is in essence a mono-allelic marker system, markers can be scored quantitatively and used to deduce zygosity. AFLP markers were generated using the enzyme combinations EcoRI/MseI and PstI/MseI. A total of 1539 and 1355 AFLP markers have been mapped in the two populations, respectively. Among the mapped PstI/MseIAFLP markers we have included fragments bounded by a methylated PstI site (mAFLP markers). Mapping these mAFLP markers shows that the presence of C-methylation segregates in perfect accordance with the primary target sequence, leading to Mendelian inheritance. Simultaneous mapping of PstI/MseIAFLP and PstI/MseI mAFLP markers allowed us to identify a number of epi-alleles, showing allelic variation in the CpNpG methylation only. However, their frequency in maize is low. Map comparison shows that, despite some rearrangements, most of the AFLP markers that are common in both populations, map at similar positions. This would indicate that AFLP markers are predominantly single-locus markers. Changes in map order occur mainly in marker-dense regions. These marker-dense regions, representing clusters of mainly EcoRI/MseI AFLP and PstI/MseI mAFLP markers, co- localize well with the putative centromeric regions of the maize chromosomes. In contrast, PstI/MseImarkers are more uniformly distributed over the genome. 相似文献
45.
Papst C Bohn M Utz HF Melchinger AE Klein D Eder J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(8):1545-1554
In hybrid breeding the performance of lines in hybrid combinations is more important than their performance per se. Little information is available on the correlation between individual line and testcross (TC) performances for the resistance to European corn borer (ECB, Ostrinia nubilalis Hb.) in maize (Zea mays L.). Marker assisted selection (MAS) will be successful only if quantitative trait loci (QTL) found in F2 derived lines for ECB resistance are still expressed in hybrid combinations. The objectives of our study were: (1) to identify and characterize QTL for ECB resistance as well as agronomic and forage quality traits in a population of testcrossed F2:3 families; (2) to evaluate the consistency of QTL for per se and TC performances; and (3) to determine the association between per se and TC performances of F2:3 lines for these traits. Two hundred and four F2:3 lines were derived from the cross between maize lines D06 (resistant) and D408 (susceptible). These lines were crossed to D171 and the TC progenies were evaluated for ECB resistance and agronomic performance in two locations in 2000 and 2001. Using these TC progenies, six QTL for stalk damage rating (SDR) were found. These QTL explained 27.4% of the genotypic variance in a simultaneous fit. Three QTL for SDR were detected consistently for per se and TC performance. Phenotypic and genotypic correlations were low for per se and TC performance for SDR. Correlations between SDR and quality traits were not significant. Based on these results, we conclude that MAS will not be an efficient method for improving SDR. However, new molecular tools might provide the opportunity to use QTL data as a first step to identify genes involved in ECB resistance. Efficient MAS procedures might then be based on markers designed to trace and to combine specific genes and their alleles in elite maize breeding germplasm.Communicated by G. Wenzel 相似文献
46.
Micic Z Hahn V Bauer E Melchinger AE Knapp SJ Tang S Schön CC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(2):233-242
Midstalk rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in sunflower (Helianthus annuus L.). Objectives of this study were to: (1) estimate the number, genomic positions and genetic effects of quantitative trait loci (QTL) for resistance to midstalk rot in line TUB-5-3234, derived from an interspecific cross; (2) determine congruency of QTL between this line and other sources of resistance; and (3) make inferences about the efficiency of selective genotyping (SG) in detecting QTL conferring midstalk rot resistance in sunflower. Phenotypic data for three resistance (stem lesion, leaf lesion and speed of fungal growth) and two morphological (leaf length and leaf length with petiole) traits were obtained from 434 F3 families from cross CM625 (susceptible) × TUB-5-3234 (resistant) under artificial infection in field experiments across two environments. The SG was applied by choosing the 60 most resistant and the 60 most susceptible F3 families for stem lesion. For genotyping of the respective F2 plants, 78 simple sequence repeat markers were used. Genotypic variances were highly significant for all traits. Heritabilities and genotypic correlations between resistance traits were moderate to high. Three to four putative QTL were detected for each resistance trait explaining between 40.8% and 72.7% of the genotypic variance (
). Two QTL for stem lesion showed large genetic effects and corroborated earlier findings from the cross NDBLOSsel (resistant) × CM625 (susceptible). Our results suggest that SG can be efficiently used for QTL detection and the analysis of congruency for resistance genes across populations. 相似文献
47.
Trends in genetic diversity among European maize cultivars and their parental components during the past 50 years 总被引:3,自引:0,他引:3
Reif JC Hamrit S Heckenberger M Schipprack W Maurer HP Bohn M Melchinger AE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(5):838-845
It has been claimed that the system that delivers the products of plant breeding reduces the diversity of cultivated varieties
leading to an increased genetic vulnerability. The main goal of our study was to monitor the temporal trends in genetic diversity
over the past five decades among maize cultivars with the largest acreage in Central Europe. Our objectives were to (1) investigate
how much of the genetic diversity present in important adapted open-pollinated varieties (OPVs) has been captured in the elite
flint germplasm pool, (2) examine changes in the genetic diversity among the most important commercial hybrids as well as
in their dent and flint parents, (3) analyze temporal changes in allele frequencies between the dent and flint parental inbreds,
and (4) investigate linkage disequilibrium (LD) trends between pairs of loci within the set of parental dent and flint lines.
We examined 30 individuals of five prominent OPVs from Central Europe, 85 maize hybrids of economic importance, and their
dent and flint parental components with 55 SSRs. LD was significant at probability level P=0.01 for 20.2% of the SSR marker pairs in the 82 dent lines and for 17.2% in the 66 flint lines. The dent and flint heterotic
groups were clearly separated already at the beginning of hybrid breeding in Central Europe. Furthermore, the genetic variation
within and among varieties decreased significantly during the five decades. The five OPVs contain numerous unique alleles
that were absent in the elite flint pool. Consequently, OPVs could present useful sources for broadening the genetic base
of elite maize breeding germplasm. 相似文献
48.
Heckenberger M Bohn M Frisch M Maurer HP Melchinger AE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(3):598-608
Genetic similarities (GS) based on molecular markers have been proposed as a tool for identification of essentially derived varieties (EDVs). Nevertheless, scientifically reliable criteria for discrimination of EDVs and independently derived varieties with GS estimates are scanty, and implementation into practical breeding has not yet taken place. Our objectives were to (1) assess the influence of chromosome number and length, marker density, and distribution, as well as the degree of polymorphism between the parental inbreds on the distribution of GS between parental inbreds and their progenies [GS(P1,O)] derived from F2 and different backcross populations and (2) evaluate these factors with regard to the power for distinguishing F2- versus BC1- and BC1- versus BC2-derived lines with molecular markers. We developed an approach based on statistical test theory for the identification of EDVs with molecular markers. Standard deviations and overlaps of distributions of GS(P1,O) of F2-, BC1-, and BC2-derived lines were smaller with (1) increasing chromosome number and length, (2) increasing marker density, and (3) uniformly instead of randomly distributed markers, approaching a lower boundary determined by the genetic parameters. The degree of polymorphism between the parental inbreds influenced the power only if the remaining number of polymorphic markers was low. Furthermore, suggestions are made for (1) determining the number of markers required to ascertain a given power and (2) EDV identification procedures. 相似文献
49.
X. Dong X. Xu J. Miao L. Li D. Zhang X. Mi C. Liu X. Tian A. E. Melchinger S. Chen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(7):1713-1720
Production of haploids by the in vivo haploid induction method has now become routine for generating new inbred lines in maize. In previous studies, a major quantitative trait locus (QTL) (qhir1) located in bin 1.04 was detected, explaining up to 66 % of the genotypic variance for haploid induction rate (HIR). Our objectives were to (1) fine-map qhir1 and (2) identify closely linked markers useful for marker-assisted breeding of new inducers. For this purpose, we screened a mapping population of 14,375 F2 plants produced from a cross between haploid inducer UH400 and non-inducer line 1680 to identify recombinants. Based on sequence information from the B73 reference genome, markers polymorphic between the two parents were developed to conduct fine mapping with these recombinants. A progeny test mapping strategy was applied to accurately determine the HIR of the 14 recombinants identified. Furthermore, F3 progeny of recombinant F2 plants were genotyped and in parallel evaluated for HIR. We corroborated earlier studies in that qhir1 has both a significantly positive effect on HIR but also a strong selective disadvantage, as indicated by significant segregation distortion. Altogether, we were able to narrow down the qhir1 locus to a 243 kb region flanked by markers X291 and X263. 相似文献
50.
Michael Stange H. Friedrich Utz Tobias A. Schrag Albrecht E. Melchinger Tobias Würschum 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(10):2563-2574
High-density genotyping is extensively exploited in genome-wide association mapping studies and genomic selection in maize. By contrast, linkage mapping studies were until now mostly based on low-density genetic maps and theoretical results suggested this to be sufficient. This raises the question, if an increase in marker density would be an overkill for linkage mapping in biparental populations, or if important QTL mapping parameters would benefit from it. In this study, we addressed this question using experimental data and a simulation based on linkage maps with marker densities of 1, 2, and 5 cM. QTL mapping was performed for six diverse traits in a biparental population with 204 doubled haploid maize lines and in a simulation study with varying QTL effects and closely linked QTL for different population sizes. Our results showed that high-density maps neither improved the QTL detection power nor the predictive power for the proportion of explained genotypic variance. By contrast, the precision of QTL localization, the precision of effect estimates of detected QTL, especially for small and medium sized QTL, as well as the power to resolve closely linked QTL profited from an increase in marker density from 5 to 1 cM. In conclusion, the higher costs for high-density genotyping are compensated for by more precise estimates of parameters relevant for knowledge-based breeding, thus making an increase in marker density for linkage mapping attractive. 相似文献