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151.
Melanie M. Davidson Ruth C. Butler David A. J. Teulon 《Journal of Insect Behavior》2012,25(3):297-307
The effect of visual cue color and size, volume of para-anisaldehyde (plant-derived semiochemical), and airflow on thrips behavior were examined in a flight chamber. After 5 min
more female Frankliniella occidentalis (western flower thrips) landed on sticky traps containing yellow plastic squares (100 cm2) (55.2% of those that flew landed on the trap) than blue (21%), white (4.7%), or transparent traps (2%). The percentage of
thrips caught on traps increased with increasing size of the visual cues (0 and 1 cm2 (4%), 4 cm2 (16%), 25 cm2 (44–49%), 100 cm2 (60%)). Using a yellow (100 cm2) square, fewer thrips flew in the presence of 1.0 ml (47%) or 2 ml (55%) of para-anisaldehyde than of 0.5 ml (78%). However, more thrips landed on a trap with a 100 cm2 yellow square when 1 ml of para-anisaldehyde (81%) was added than when 0.5 ml (55%) or 2 ml (62%) were added. Airflow (0–0.3 m/s) did not affect the percentage
of thrips that flew or landed on traps. Results suggest that thrips responded to a yellow cue in the absence of UV. Further,
the volume of para-anisladehyde affected the percentage that flew or landed on a trap containing a yellow cue. 相似文献
152.
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154.
Jünger M Vautz W Kuhns M Hofmann L Ulbricht S Baumbach JI Quintel M Perl T 《Applied microbiology and biotechnology》2012,93(6):2603-2614
Presently, 2 to 4 days elapse between sampling at infection suspicion and result of microbial diagnostics. This delay for
the identification of pathogens causes quite often a late and/or inappropriate initiation of therapy for patients suffering
from infections. Bad outcome and high hospitalization costs are the consequences of these currently existing limited pathogen
identification possibilities. For this reason, we aimed to apply the innovative method multi-capillary column–ion mobility
spectrometry (MCC-IMS) for a fast identification of human pathogenic bacteria by determination of their characteristic volatile
metabolomes. We determined volatile organic compound (VOC) patterns in headspace of 15 human pathogenic bacteria, which were
grown for 24 h on Columbia blood agar plates. Besides MCC-IMS determination, we also used thermal desorption–gas chromatography–mass
spectrometry measurements to confirm and evaluate obtained MCC-IMS data and if possible to assign volatile compounds to unknown
MCC-IMS signals. Up to 21 specific signals have been determined by MCC-IMS for Proteus mirabilis possessing the most VOCs of all investigated strains. Of particular importance is the result that all investigated strains
showed different VOC patterns by MCC-IMS using positive and negative ion mode for every single strain. Thus, the discrimination
of investigated bacteria is possible by detection of their volatile organic compounds in the chosen experimental setup with
the fast and cost-effective method MCC-IMS. In a hospital routine, this method could enable the identification of pathogens
already after 24 h with the consequence that a specific therapy could be initiated significantly earlier. 相似文献
155.
A Zahs MD Bird L Ramirez JR Turner MA Choudhry EJ Kovacs 《American journal of physiology. Gastrointestinal and liver physiology》2012,303(6):G705-G712
Laboratory evidence suggests that intestinal permeability is elevated following either binge ethanol exposure or burn injury alone, and this barrier dysfunction is further perturbed when these insults are combined. We and others have previously reported a rise in both systemic and local proinflammatory cytokine production in mice after the combined insult. Knowing that long myosin light-chain kinase (MLCK) is important for epithelial barrier maintenance and can be activated by proinflammatory cytokines, we examined whether inhibition of MLCK alleviated detrimental intestinal responses seen after ethanol exposure and burn injury. To accomplish this, mice were given vehicle or a single binge ethanol exposure followed by a sham or dorsal scald burn injury. Following injury, one group of mice received membrane permeant inhibitor of MLCK (PIK). At 6 and 24 h postinjury, bacterial translocation and intestinal levels of proinflammatory cytokines were measured, and changes in tight junction protein localization and total intestinal morphology were analyzed. Elevated morphological damage, ileal IL-1β and IL-6 levels, and bacterial translocation were seen in mice exposed to ethanol and burn injury relative to either insult alone. This increase was not seen in mice receiving PIK after injury. Ethanol-exposed and burn-injured mice had reduced zonula occludens protein-1 and occludin localization to the tight junction relative to sham-injured mice. However, the observed changes in junctional complexes were not seen in our PIK-treated mice following the combined insult. These data suggest that MLCK activity may promote morphological and inflammatory responses in the ileum following ethanol exposure and burn injury. 相似文献
156.
Crossovers (COs) between homologous chromosomes ensure their faithful segregation during meiosis. We identify C.?elegans COSA-1, a cyclin-related protein conserved in metazoa, as a key component required to convert meiotic double-strand breaks (DSBs) into COs. During late meiotic prophase, COSA-1 localizes to foci that correspond to the single CO site on each homolog pair and indicate sites of eventual concentration of other conserved CO proteins. Chromosomes gain and lose competence to load CO proteins during meiotic progression, with competence to load COSA-1 requiring prior licensing. Our data further suggest a self-reinforcing mechanism maintaining CO designation. Modeling of a nonlinear dose-response relationship between IR-induced DSBs and COSA-1 foci reveals efficient conversion of DSBs into COs when DSBs are limiting and a robust capacity to limit cytologically differentiated CO sites when DSBs are in excess. COSA-1 foci serve as a unique live cell readout for investigating CO formation and CO interference. 相似文献
157.
158.
Wallis IR Edwards MJ Windley H Krockenberger AK Felton A Quenzer M Ganzhorn JU Foley WJ 《Oecologia》2012,169(2):281-291
Ecologists want to explain why populations of animals are not evenly distributed across landscapes and often turn to nutritional explanations. In seeking to link population attributes with food quality, they often contrast nutritionally positive traits, such as the concentration of nitrogen, against negative ones, such as fibre concentration, by using a ratio of these traits. This specific ratio has attracted attention because it sometimes correlates with the biomass of colobine primates across sites in Asia and Africa. Although empirically successful, we have identified problems with the ratio that may explain why it fails under some conditions to predict colobine biomass. First, available nitrogen, rather than total nitrogen, is nutritionally important, while the presence of tannins is the major factor reducing the availability of nitrogen in browse plant species. Second, tannin complexes inflate measures of fibre. Finally, simple ratios may be unsound statistically because they implicitly assume isometric relationships between variables. We used data on the chemical composition of plants from three continents to examine the relationships between the concentrations of nitrogen, available nitrogen, fibre and tannins in foliage and to evaluate the nitrogen to fibre ratio. Our results suggest that the ratio of the concentration of nitrogen to fibre in leaves does sometimes closely correlate with the concentration of available nitrogen. However, the ratio may give misleading results when leaves contain high concentrations of tannins. The concentration of available nitrogen, which incorporates measures of total nitrogen, dry matter digestibility and tannins, should give a better indication of the nutritional value of leaves for herbivorous mammals that can readily be extrapolated to habitats. 相似文献
159.
Dammhahn M 《Proceedings. Biological sciences / The Royal Society》2012,279(1738):2645-2651
Despite increasing interest, animal personality is still a puzzling phenomenon. Several theoretical models have been proposed to explain intraindividual consistency and interindividual variation in behaviour, which have been primarily supported by qualitative data and simulations. Using an empirical approach, I tested predictions of one main life-history hypothesis, which posits that consistent individual differences in behaviour are favoured by a trade-off between current and future reproduction. Data on life-history were collected for individuals of a natural population of grey mouse lemurs (Microcebus murinus). Using open-field and novel-object tests, I quantified variation in activity, exploration and boldness for 117 individuals over 3 years. I found systematic variation in boldness between individuals of different residual reproductive value. Young males with low current but high expected future fitness were less bold than older males with high current fecundity, and males might increase in boldness with age. Females have low variation in assets and in boldness with age. Body condition was not related to boldness and only explained marginal variation in exploration. Overall, these data indicate that a trade-off between current and future reproduction might maintain personality variation in mouse lemurs, and thus provide empirical support of this life-history trade-off hypothesis. 相似文献
160.
Zuo W Moses ME West GB Hou C Brown JH 《Proceedings. Biological sciences / The Royal Society》2012,279(1734):1840-1846
The temperature size rule (TSR) is the tendency for ectotherms to develop faster but mature at smaller body sizes at higher temperatures. It can be explained by a simple model in which the rate of growth or biomass accumulation and the rate of development have different temperature dependence. The model accounts for both TSR and the less frequently observed reverse-TSR, predicts the fraction of energy allocated to maintenance and synthesis over the course of development, and also predicts that less total energy is expended when developing at warmer temperatures for TSR and vice versa for reverse-TSR. It has important implications for effects of climate change on ectothermic animals. 相似文献