全文获取类型
收费全文 | 159篇 |
免费 | 23篇 |
专业分类
182篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 3篇 |
2019年 | 5篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 8篇 |
2014年 | 22篇 |
2013年 | 11篇 |
2012年 | 9篇 |
2011年 | 15篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 6篇 |
2007年 | 6篇 |
2006年 | 10篇 |
2005年 | 5篇 |
2004年 | 5篇 |
2003年 | 3篇 |
2002年 | 6篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 1篇 |
1992年 | 2篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1974年 | 3篇 |
1969年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有182条查询结果,搜索用时 15 毫秒
41.
42.
Yagahira E. Castro-Sesquen Robert H. Gilman Carolina Mejia Daniel E. Clark Jeong Choi Melissa J. Reimer-McAtee Rosario Castro Edward Valencia-Ayala Jorge Flores Natalie Bowman Ricardo Castillo-Neyra Faustino Torrico Lance Liotta Caryn Bern Alessandra Luchini The Chagas/HIV Working Group in Bolivia Peru 《PLoS neglected tropical diseases》2016,10(2)
Background
Early diagnosis of reactivated Chagas disease in HIV patients could be lifesaving. In Latin America, the diagnosis is made by microscopical detection of the T. cruzi parasite in the blood; a diagnostic test that lacks sensitivity. This study evaluates if levels of T. cruzi antigens in urine, determined by Chunap (Chagas urine nanoparticle test), are correlated with parasitemia levels in T. cruzi/HIV co-infected patients.Methodology/Principal Findings
T. cruzi antigens in urine of HIV patients (N = 55: 31 T. cruzi infected and 24 T. cruzi serology negative) were concentrated using hydrogel particles and quantified by Western Blot and a calibration curve. Reactivation of Chagas disease was defined by the observation of parasites in blood by microscopy. Parasitemia levels in patients with serology positive for Chagas disease were classified as follows: High parasitemia or reactivation of Chagas disease (detectable parasitemia by microscopy), moderate parasitemia (undetectable by microscopy but detectable by qPCR), and negative parasitemia (undetectable by microscopy and qPCR). The percentage of positive results detected by Chunap was: 100% (7/7) in cases of reactivation, 91.7% (11/12) in cases of moderate parasitemia, and 41.7% (5/12) in cases of negative parasitemia. Chunap specificity was found to be 91.7%. Linear regression analysis demonstrated a direct relationship between parasitemia levels and urine T. cruzi antigen concentrations (p<0.001). A cut-off of > 105 pg was chosen to determine patients with reactivation of Chagas disease (7/7). Antigenuria levels were 36.08 times (95% CI: 7.28 to 64.88) higher in patients with CD4+ lymphocyte counts below 200/mL (p = 0.016). No significant differences were found in HIV loads and CD8+ lymphocyte counts.Conclusion
Chunap shows potential for early detection of Chagas reactivation. With appropriate adaptation, this diagnostic test can be used to monitor Chagas disease status in T. cruzi/HIV co-infected patients. 相似文献43.
44.
Protein acetylation affects acetate metabolism,motility and acid stress response in
Escherichia coli 下载免费PDF全文
45.
46.
Victoria V. Abzianidze Sofya A. Zakharenkova Natalia I. Moiseeva Petr P. Beltyukov Valeriy A. Polukeev Yaroslav A. Dubrovskii Victor A. Kuznetsov Yuri G. Trishin Jennifer E. Mejia Alvin A. Holder 《Bioorganic & medicinal chemistry letters》2019,29(1):59-61
New derivatives of phaeosphaeride A (PPA) were synthesized and characterized. Anti-tumor studies were carried out on the U937, HCT-116, PC3, MCF-7, A549, К562, NCI-H929, Jurkat, THP-1, RPMI8228 tumor cell lines, and on the HEF cell line. All the compounds synthesized were found to have better efficacy than PPA towards the tumor cell lines mentioned. Compound 6 (IC50?=?0.59?±?0.27?µM) was observed to be 11 times more active than PPA (IC50?=?6.5?±?0.30?µM) towards the NCI-H929 cell line, with a therapeutic index of 18. Compound 6 was determined to be over half and 16 times more active than etoposide towards the NCI-H929 (IC50?=?0.9?±?0.05?µM) and A549 (IC50?=?100?±?7.0?µM) cell lines, respectively. 相似文献
47.
48.
Reconstruction of the eyelids can range from simple repair to the integration of multiple complex procedures. Knowledge of eyelid anatomy, adequate preoperative planning, and meticulous surgical technique will optimize the anatomical and functional result. The purpose of this article is to review the relevant anatomy for eyelid reconstruction, to simplify defect analysis and preoperative planning, and to provide options for reconstruction of this complex area. 相似文献
49.
Francine H. Mejia Valerie Ouellet Martin A. Briggs Stephanie M. Carlson Roser Casas-Mulet Mollie Chapman Mathias J. Collins Stephen J. Dugdale Joseph L. Ebersole Danielle M. Frechette Aimee H. Fullerton Carole-Anne Gillis Zachary C. Johnson Christa Kelleher Barret L. Kurylyk Rebecca Lave Benjamin H. Letcher Knut M. Myrvold Tracie-Lynn Nadeau Helen Neville Herve Piégay Kathryn A. Smith Diego Tonolla Christian E. Torgersen 《Global Change Biology》2023,29(19):5482-5508
Human activities and climate change threaten coldwater organisms in freshwater ecosystems by causing rivers and streams to warm, increasing the intensity and frequency of warm temperature events, and reducing thermal heterogeneity. Cold-water refuges are discrete patches of relatively cool water that are used by coldwater organisms for thermal relief and short-term survival. Globally, cohesive management approaches are needed that consider interlinked physical, biological, and social factors of cold-water refuges. We review current understanding of cold-water refuges, identify gaps between science and management, and evaluate policies aimed at protecting thermally sensitive species. Existing policies include designating cold-water habitats, restricting fishing during warm periods, and implementing threshold temperature standards or guidelines. However, these policies are rare and uncoordinated across spatial scales and often do not consider input from Indigenous peoples. We propose that cold-water refuges be managed as distinct operational landscape units, which provide a social and ecological context that is relevant at the watershed scale. These operational landscape units provide the foundation for an integrated framework that links science and management by (1) mapping and characterizing cold-water refuges to prioritize management and conservation actions, (2) leveraging existing and new policies, (3) improving coordination across jurisdictions, and (4) implementing adaptive management practices across scales. Our findings show that while there are many opportunities for scientific advancement, the current state of the sciences is sufficient to inform policy and management. Our proposed framework provides a path forward for managing and protecting cold-water refuges using existing and new policies to protect coldwater organisms in the face of global change. 相似文献
50.
William A. Taylor Edgard M. Mejia Ryan W. Mitchell Patrick C. Choy Genevieve C. Sparagna Grant M. Hatch 《PloS one》2012,7(11)
Cardiolipin (CL) is a mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes involved in the generation of ATP. In order to facilitate its role CL must be remodeled with appropriate fatty acids. We previously identified a human monolysocardiolipin acyltransferase activity which remodels CL via acylation of monolysocardiolipin (MLCL) to CL and was identical to the alpha subunit of trifunctional protein (αTFP) lacking the first 227 amino acids. Full length αTFP is an enzyme that plays a prominent role in mitochondrial β-oxidation, and in this study we assessed the role, if any, which this metabolic enzyme plays in the remodeling of CL. Purified human recombinant αTFP exhibited acyl-CoA acyltransferase activity in the acylation of MLCL to CL with linoleoyl-CoA, oleoyl-CoA and palmitoyl-CoA as substrates. Expression of αTFP increased radioactive linoleate or oleate or palmitate incorporation into CL in HeLa cells. Expression of αTFP in Barth Syndrome lymphoblasts, which exhibit reduced tetralinoleoyl-CL, elevated linoleoyl-CoA acylation of MLCL to CL in vitro, increased mitochondrial respiratory Complex proteins and increased linoleate-containing species of CL. Knock down of αTFP in Barth Syndrome lymphoblasts resulted in greater accumulation of MLCL than those with normal αTFP levels. The results clearly indicate that the human αTFP exhibits MLCL acyltransferase activity for the resynthesis of CL from MLCL and directly links an enzyme of mitochondrial β-oxidation to CL remodeling. 相似文献