Comments in a Discussion: Differential Rates of d- and l-tyrosine Crystallization

We earlier reported that we had observed quantifiable differences in crystallization rates of D and L tyrosine. It has been suggested that these results were due to the presence of impurities. Here we argue that it is premature to conclude that impurities entirely explain the results. More generally, there is an accumulating weight of evidence that D and L enantiomers display unexpected differences in their physical properties and behavior. These should be taken into account as we attempt to understand the origin of biochirality.     

Isolation of N,N-dialkylated derivatives of valproylglycinamide from dog plasma by active charcoal adsorption and their quantification by high-performance liquid chromatography

A selective assay for quantification of N,N-dimethylvalproylglycinamide (DM-VGD) and N,N-diethylvalproylglycinamide (DE-VGD) in dog plasma utilizing reversed-phase high-performance liquid chromatography and UV detection has been developed. These compounds are derivatives of the potential anticonvulsant drug, valproylglycinamide, which is currently undergoing clinical trials. The method is based on extraction of dog plasma with activated charcoal, separation of the charcoal pallet and extracting it with methanol, evaporation of the solvent and injecting the reconstituted residue onto the column. The active charcoal adsorption method is reliable and reproducible, and it provides a chromatogram free of interfering endogenous plasma compounds. The assay was validated and provided a limit of quantification of 2.3 mmol/l for DE-VGD and 5.3 mmol/l for DM-VGD. Mean recovery of these compounds from plasma averages 75%. This analytical method is suitable for the quantitative determination of DM-VGD and DE-VGD in plasma and it has been applied to a pharmacokinetic study of these compounds in a dog.     

Seedling growth declines in warmed tropical forest soils

The response of plants to a warming climate could have a large feedback on further climatic change. This feedback is especially important for tropical forests, where the global peak in plant productivity and biodiversity occurs. Here we test the response of tropical forest tree seedling growth, photosynthesis and herbivory to 3 years of in situ full-soil profile warming. We studied six species, three of which are known nitrogen-fixers and we hypothesized that the warming response of growth will be mediated by nutrient availability to plants. Across species, growth was significantly lower in warmed soil compared to soil at ambient temperature, and the same pattern was observed for light-saturated photosynthesis, pointing toward a growth decline associated with decreased C fixation. Within species, the relative growth decline was significant for two species, Inga laurina and Tachigali versicolor, both of which are N-fixers. Together our results suggest a growth decline may have resulted from a negative effect of warming on N-fixation, rather than via changes in nutrient mineralization from soil organic matter, which was unchanged for N and increased for P during the dry-to-wet season transition. Overall, our study demonstrates that belowground warming causes species-specific declines in the growth and photosynthesis of seedlings, with a suggestion—requiring further investigation—that this growth decline is larger in N-fixing species.     

Relationship between suicide and myocardial infarction with regard to changing physical environmental conditions

In recent years, the possible association of changes in mortality from cardiovascular disease and myocardial infarction (MI) and deaths related to violence and the suicide rate has been repeatedly discussed. This study examined the relationship between cosmic physical changes (solar, geomagnetic and other space activity parameters) and changes in the total number of in-hospital and MI-related deaths and deaths from suicide to determine if a relationship exists between the distribution of total and MI-related deaths with suicide over time; some differences in the serotonergic mechanisms involved in the pathogenesis of MI and suicide were also taken into account. All suicides (n=2359) registered in the State of Israel from 1981 to 1989 (108 months) were analysed and compared with the total number of deaths (n=15601) and deaths from MI (n=1573) in a large university hospital over 180 months (1974–1989). The following were the main features of the Results. (1) Monthly suicide rate was correlated with space proton flux (r=0.42,P=0.0001) and with geomagnetic activity (r=–0.22,P=0.03). (2) Total hospital and MI-related deaths were correlated with solar activity parameters (r=0.35,P<0.001) and radiowave propagation (r=0.52-0.44,P<0.001), an with proton flux (r=–0.3 to –0.26,P<0.01). (3) Monthly suicide distribution over 108 months was correlated with MI (r=–0.33,P=0.0005) and total hospital mortality (r=–0.22,P=0.024). (4) Gender differences were prominent. We conclude that the monthly distributions of suicides and deaths from MI are adversely related to many environmental physical parameters and negatively correlated with each other.     

Does conservation planning matter in a dynamic and uncertain world?

Loss of biodiversity is one of the world's overriding environmental challenges. Reducing those losses by creating reserve networks is a cornerstone of global conservation and resource management. Historically, assembly of reserve networks has been ad hoc, but recently the focus has shifted to identifying optimal reserve networks. We show that while comprehensive reserve network design is best when the entire network can be implemented immediately, when conservation investments must be staged over years, such solutions actually may be sub‐optimal in the context of biodiversity loss and uncertainty. Simple decision rules, such as protecting the available site with the highest irreplaceability or with the highest species richness, may be more effective when implementation occurs over many years.     

Automatic noninvasive measurement of systolic blood pressure using photoplethysmography

Background  

Automatic measurement of arterial blood pressure is important, but the available commercial automatic blood pressure meters, mostly based on oscillometry, are of low accuracy.     

Improved Bacterial Detection Using Immobilized Acyl-Lysyl Oligomers

The global need to improve bacterial detection in liquid media has motivated multidisciplinary research efforts toward developing new approaches that overcome the shortcomings of traditional techniques. We recently proposed the use of oligomers of acylated lysyls (OAKs) in their resin-linked form (ROAKs) for the efficient, robust, and inexpensive filtration of bacteria. Here, to investigate the potential for the use of ROAKs in downstream applications, we first examined the capacity of ROAKs to capture bacteria as a function of environmental conditions and structure-activity relationships (SARs). We next assessed their ability to release the captured bacteria and then combined both abilities to improve real-time PCR outcomes. ROAKs were able to deplete liquid samples of bacterial content after incubation or continuous flow, illustrating the efficient capture of different bacterial species under a wide range of ionic strength and pH conditions. We also show circumstances for the significant release of captured bacteria, live or dead, for further analysis. Finally, the SAR study revealed a shorter ROAK derivative exhibiting a capture capacity similar to that of the parent construct but the increased recovery of ROAK-bound bacteria, enabling improvement of the detection sensitivity by 20-fold. Collectively, the data support the potential usefulness of a simple, robust, and efficient approach for rapid capture/analysis of bacteria from tap water and, possibly, from more complex media.