全文获取类型
收费全文 | 965篇 |
免费 | 95篇 |
出版年
2022年 | 5篇 |
2021年 | 11篇 |
2019年 | 6篇 |
2018年 | 15篇 |
2017年 | 5篇 |
2016年 | 9篇 |
2015年 | 29篇 |
2014年 | 36篇 |
2013年 | 39篇 |
2012年 | 35篇 |
2011年 | 41篇 |
2010年 | 33篇 |
2009年 | 26篇 |
2008年 | 29篇 |
2007年 | 43篇 |
2006年 | 39篇 |
2005年 | 41篇 |
2004年 | 37篇 |
2003年 | 34篇 |
2002年 | 34篇 |
2001年 | 40篇 |
2000年 | 45篇 |
1999年 | 24篇 |
1998年 | 11篇 |
1997年 | 20篇 |
1996年 | 14篇 |
1995年 | 15篇 |
1994年 | 9篇 |
1993年 | 12篇 |
1992年 | 22篇 |
1991年 | 23篇 |
1990年 | 22篇 |
1989年 | 24篇 |
1988年 | 29篇 |
1987年 | 21篇 |
1986年 | 13篇 |
1985年 | 12篇 |
1984年 | 16篇 |
1983年 | 11篇 |
1982年 | 11篇 |
1981年 | 9篇 |
1980年 | 17篇 |
1979年 | 16篇 |
1978年 | 13篇 |
1977年 | 8篇 |
1976年 | 8篇 |
1975年 | 7篇 |
1974年 | 7篇 |
1973年 | 4篇 |
1970年 | 7篇 |
排序方式: 共有1060条查询结果,搜索用时 109 毫秒
191.
Design, synthesis, and testing of an 6-O-linked series of benzimidazole based inhibitors of CDK5/p25
Jain P Flaherty PT Yi S Chopra I Bleasdell G Lipay J Ferandin Y Meijer L Madura JD 《Bioorganic & medicinal chemistry》2011,19(1):359-373
Alzheimer’s disease (AD) is a progressive neurodegenerative disease resulting in cognitive and behavioral impairment. The two classic pathological hallmarks of AD include extraneuronal deposition of amyloid ?? (A??) and intraneuronal formation of neurofibrillary tangles (NFTs). NFTs contain hyperphosphorylated tau. Tau is the major microtubule-associated protein in neurons and stabilizes microtubules (MTs). Cyclin dependent kinase 5 (CDK5), when activated by the regulatory binding protein p25, phosphorylates tau at a number of proline-directed serine/threonine residues, resulting in formation of phosphorylated tau as paired helical filaments (PHFs) then in subsequent deposition of PHFs as NFTs. Beginning with the structure of Roscovitine, a moderately selective CDK5 inhibitor, we sought to conduct structural modifications to increase inhibitory potency of CDK5 and increase selectivity over a similar enzyme, cyclin dependent kinase 2 (CDK2). The design, synthesis, and testing of a series of 1-isopropyl-4-aminobenzyl-6-ether-linked benzimidazoles is presented. 相似文献
192.
A. B. P. van Kuilenburg J. Meijer G. Gökcay T. Baykal M. E. Rubio-Gozalbo A. N. P. M. Mul 《Nucleosides, nucleotides & nucleic acids》2013,32(4-6):509-514
Dihydropyrimidine dehydrogenase (DPD) deficiency is an autosomal recessive disorder of the pyrimidine degradation pathway. In a patient presenting with convulsions, psychomotor retardation and Reye like syndrome, strongly elevated levels of uracil and thymine were detected in urine. No DPD activity could be detected in peripheral blood mononuclear cells. Analysis of the gene encoding DPD (DPYD) showed that the patient was homozygous for a novel c.505_513del (p.169_171del) mutation in exon 6 of DPYD. 相似文献
193.
Per-Johan Meijer Gösta Lilius Niklas Holmberg Leif Bülow 《Biotechnology letters》1996,18(10):1133-1138
Summary An artificial bifunctional enzyme, -glutamyl kinase/-glutamyl phosphate reductase, was obtained by fusing the Escherichia coli genes proA and proB. The proB gene was fused to the 5-end of the proA gene with a linker encoding five amino acids. When expressed in E. coli enhanced intracellular concentrations of proline were observed. At 0.6 M NaCl the growth rates for the strain carrying the fusion enzyme and a control harbouring a plasmid encoding the wild-type enzymes were 320 and 530 min, respectively. 相似文献
194.
Harold J. G. Meijer Nullin Divecha Herman van den Ende Alan Musgrave Teun Munnik 《Planta》1999,208(2):294-298
Cells from several different plant species synthesised a polyphosphoinositide (PPI)-like lipid when osmo-stressed. Synthesis
was maximal after about 10 min and was stimulated by a variety of osmolytes. Using NaCl, the strongest response centred around
200 mM. The lipid was shown to be the novel PPI isomer phosphatidyl-inositol 3,5-bisphosphate [PtdIns-(3,5)P2] by analytical thin-layer chromatography and conversion to PtdIns(3,4,5)P3 using recombinant phosphoinositide 4-OH kinase. The results indicate that PtdIns-(3,5)P2 plays a role in a general osmo-signalling pathway in plants. Its potential role is discussed.
Received: 6 November 1998 / Accepted: 14 December 1998 相似文献
195.
196.
197.
Development of fish communities in lakes after biomanipulation 总被引:2,自引:0,他引:2
M. -L. Meijer E. H. R. R. Lammens A. J. P. Raat J. G. P. Klein Breteler M. P. Grimm 《Aquatic Ecology》1995,29(1):91-101
Biomanipulation measures in the Netherlands are usually a combination of a drastic fish stock reduction and an introduction of pike fingerlings. In three small shallow lakes (Noorddiep, Bleiswijkse Zoom and Zwemlust) these measures resulted in a clear water state and the development of macrophytes. After the measures the fish community developed differently because of the new physical and biological conditions. Results of lake Noorddiep and lake Bleiswijkse Zoom showed that the fish community became more divers. Bream and carp became less dominant and were partly replaced by roach and perch. The importance of the main predator pike-perch was strongly reduced and replaced by pike and perch. The share of piscivorous fish in the total fish stock increased at all sites. The recruitment of young-of-the-year was similar or even higher in the clear overgrown areas than in the turbid water before the measures, but the recruitment of young-of-the-year to older fish differed between the species. Predation by pike and perch could not control the young-of-the-year cyprinids, but their predation may have contributed to the shift from bream to roach, because of selective predation on bream in the open water, while roach was hiding in the vegetation. The macrophytes provide new refugia and feeding conditions that favour roach and perch, but offer relatively poor survival conditions for bream and carp. 相似文献
198.
Schang LM Bantly A Knockaert M Shaheen F Meijer L Malim MH Gray NS Schaffer PA 《Journal of virology》2002,76(15):7874-7882
Pharmacological cyclin-dependent kinase (cdk) inhibitors (PCIs) block replication of several viruses, including herpes simplex virus type 1 (HSV-1) and human immunodeficiency virus type 1 (HIV-1). Yet, these antiviral effects could result from inhibition of either cellular cdks or viral enzymes. For example, in addition to cellular cdks, PCIs could inhibit any of the herpesvirus-encoded kinases, DNA replication proteins, or proteins involved in nucleotide metabolism. To address this issue, we asked whether purine-derived PCIs (P-PCIs) inhibit HSV and HIV-1 replication by targeting cellular or viral proteins. P-PCIs inhibited replication of HSV-1 and -2 and HIV-1, which require cellular cdks to replicate, but not vaccinia virus or lymphocytic choriomeningitis virus, which are not known to require cdks to replicate. P-PCIs also inhibited strains of HSV-1 and HIV-1 that are resistant to conventional antiviral drugs, which target viral proteins. In addition, the anti-HSV effects of P-PCIs and a conventional antiherpesvirus drug, acyclovir, were additive, demonstrating that the two drugs act by distinct mechanisms. Lastly, the spectrum of proteins that bound to P-PCIs in extracts of mock- and HSV-infected cells was the same. Based on these observations, we conclude that P-PCIs inhibit virus replication by targeting cellular, not viral, proteins. 相似文献
199.
Molecular Evidence for Association of Chlamydiales Bacteria with Epitheliocystis in Leafy Seadragon (Phycodurus eques), Silver Perch (Bidyanus bidyanus), and Barramundi (Lates calcarifer) 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Adam Meijer Paul J. M. Roholl Jacobus M. Ossewaarde Brian Jones Barbara F. Nowak 《Applied microbiology》2006,72(1):284-290
Epitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer), previously associated with chlamydial bacterial infection using ultrastructural analysis, was further investigated by using molecular and immunocytochemical methods. Morphologically, all three species showed epitheliocystis cysts in the gills, and barramundi also showed lymphocystis cysts in the skin. From gill cysts of all three species and from skin cysts of barramundi 16S rRNA gene fragments were amplified by PCR and sequenced, which clustered by phylogenetic analysis together with other chlamydia-like organisms in the order Chlamydiales in a lineage separate from the family Chlamydiaceae. By using in situ RNA hybridization, 16S rRNA Chlamydiales-specific sequences were detected in gill cysts of silver perch and in gill and skin cysts of barramundi. By applying immunocytochemistry, chlamydial antigens (lipopolysaccharide and/or membrane protein) were detected in gill cysts of leafy seadragon and in gill and skin cysts of barramundi, but not in gill cysts of silver perch. In conclusion, this is the first time epitheliocystis agents of leafy seadragon, silver perch and barramundi have been undoubtedly identified as belonging to bacteria of the order Chlamydiales by molecular methods. In addition, the results suggested that lymphocystis cysts, known to be caused by iridovirus infection, could be coinfected with the epitheliocystis agent. 相似文献
200.
D H?ussinger S Soboll A J Meijer W Gerok J M Tager H Sies 《European journal of biochemistry》1985,152(3):597-603
In livers of fed rats and in perfused livers supplied with a physiological portal glutamine concentration of 0.6 mM, the mitochondrial and cytosolic glutamine concentrations are 20 mM and 7 mM, respectively, thus, the mitochondrial/cytosolic glutamine concentration gradient is 2-3. Uptake and release of glutamine by periportal and perivenous hepatocytes occurs predominantly by an Na+-dependent transport system (so-called system 'N'). Histidine in near-physiological concentrations inhibits both glutamine uptake by periportal hepatocytes and its release by perivenous hepatocytes. This is not due to an inhibition of glutamine-metabolizing enzymes by histidine or its metabolites. With physiological portal glutamine concentrations (0.6 mM), stimulation of glutaminase flux or of glutamine transaminase flux is followed by a decrease of hepatic glutamine levels to about 80% or 30%, respectively, glutamine levels are further decreased to 50% or 20% in the presence of histidine. When glutamine is synthesized endogenously (no glutamine added), the histidine-induced inhibition of glutamine release is paralleled by a 210% increase of the hepatic tissue level of glutamine. In experiments with and without methionine sulfoximine and in the absence of added glutamine, the glutamine content in the small perivenous hepatocyte population containing glutamine synthetase is estimated to be about 3.5 mumol/g wet weight and that in the periportal hepatocytes as low as 0.1 mumol/g wet weight. In contrast to the prevailing view, it is concluded that glutamine transport across the plasma membrane of hepatocytes is a potential regulatory site in glutamine degradation and synthesis, especially under the influence of effectors like histidine. 相似文献