河南新乡地区儿童头面部测量

本文对河南省新乡地区汉族儿童（４－１３岁）头面部进行了测量,比较和分析了儿童体质发育与年龄增长的关系,据儿童头面部各指数数值大小分型,确定该地区汉族儿童面部的形态为：圆头型、高头型、狭头型、狭面型、狭鼻型。     

氧化应激与cfos和cjun转录和AP－1激活

激活剂蛋白-1(activator protein-1,AP-1)是近年来受到关注的与氧化应激基因表达调控有关的转录因子.文章就氧化应激与cfos和cjun基因表达,AP-1的激活,AP-1对氧化应激反应的调控,AP-1与亲电子反应元件等有关内容作了简要的综述．     

Fractionation of primary amyloid fibrils. Characterization and chemical interaction of the subunits.

Amyloid fibrils of kappa origin from a patient with primary amyloidosis are dissociated in various denaturants and fractionated into their subunit components on Sepharose 6B. Solubilization of the fibrils in 4 M guanidine-HCl followed by reduction and alkylation produced 22 000 and 17 000 dalton fractions. Without prior reduction and alkylation, these fractions exist as a high molecular weight protein which can be separated on Sepharose 6B. A high molecular weight protein can be directly dissociated from the amyloid fibril with 1% sodium dodecyl sulfate or 1 M NaCl. Reduction and alkylation of this material produces the two lower molecular weight fractions, i.e., 22 000 and 17 000. These have in the first 20 residues identical N-terminal amino acid sequences; they share immunologic identity and have similar tryptic peptide map profiles. Amino acid analysis of the 22 000 dalton fraction is identical with the intact immunoglobulin light chain isolated from the patient's serum. These data suggest that the insoluble amyloid fibril is the result of aggregation by disulfide linkages between the 22 000 and 17 000 dalton fractions.     

Studies of adult amphibian heart cells in Vitro: DNA synthesis and mitosis

The ventricle of the adult newt heart was excised and cut into several pieces of approximately 0.5 – 1.0 mm. These heart pieces were then cultured for 60 days at 25 °C in a modified Leibovitz medium (L-15). Approximately 37% of the explants were attached to the substrate and more than 33% of the attached explants and approximately 15% of the unattached explants established pulsation rates which ranged 3–67 beats/min. The explants were labeled with 1 μCi/ml of ³H-thymidine for 24 hr at 7, 15, 21, 30, 45 and 60 days of culture initiation, and processed for electron microscopic autoradiography. The examination of the autoradiograms revealed that as the culture continued, the cardiac muscle cells altered their morphology, resembling embryonic cardiac muscle cells. These altered muscle cells were termed dedifferentiated cardiac muscle cells. The number of these dedifferentiated cells increased over the period of culture, showing 10.3–94% dedifferentiated cells after 7–60 days of culture respectively. DNA synthesis and mitosis were observed in the dedifferentiated cardiac muscle cells, apart from the non-muscle cells. The quantitation of the autoradiograms revealed that the number of labeled nuclei in the cardiac muscle cells gradually increased over the period of culture, and a maximum number of labeled cardiac muscle cells (30%) was observed in the third week. The peak was followed by a decline in the eighth week which exhibited 1.5 % labeled cardiac muscle cells. The trend of mitosis was similar to that of DNA synthesis. The maximum number of mitotic figures (9%) was observed in the third week of culture, which was followed by a decline and finally absent in the eighth week. The cardiac non-muscle cells, mostly fibroblasts and endothelial cells, also showed incorporation of ³H-thymidine in their nuclei. The number of labeled non-muscle cells nuclei and the mitotic index were highest (61 and 15% respectively) in the first week of culture, but then they decreased gradually over the eight-week period in culture. This study provides evidence for the first time that the adult amphibian cardiac myocytes can undergo DNA synthesis and mitosis when explanted and cultured. The significance of this cell replication is discussed.     

Sensory innervation of the gastro-intestinal junction: new electrophysiological, histological and histochemical data]

The sensory innervation of the small intestine was studied in the cat with electrophysiological, histological and histochemical techniques. Thanks to the histochemical technique (peroxydase method) the exact number and proportion of splanchnic and vagal fibres was determined : the latter being about 9 times more numerous than the former. On the other hand the exact position of the corresponding cells was defined in the nodose and spinal ganglia by means of the previous technique and the microelectrophysiological method (recording of single units into the ganglia with extracellular glass microelectrodes). The splanchnic neurones were found in the T9, T10 and T11 ganglia whereas the vagal ones were chiefly located in the lower half of the nodose ganglia. The histological studies using electronic microscope showed many non-medullated endings, which were often found beneath the epithelium and in the lamina propria of the villi close to the blood vessels. This result is certainly the proof that numerous receptors (mechanoreceptors, chemoreceptors and even thermoreceptors do exist in the small intestine.     

武汉地区的水绵

作者自1965年开始即从事湖北省双星藻科植物标本的采集。在武汉地区采得水绵属(Spirogyra)标本中,鉴定了28个种和两个变型。其中有5个种(扩大水绵,双形水绵,长圆水绵,伪美丽水绵,武昌水绵)和两个变型(泡状水绵小形变型,网纹水绵柱孢变型)在目前可以认为是武汉地区特有的种;有5个种(泡状水绵,陈氏水绵,河北水绵,丘疹水绵,青岛水绵)是我国的特有种;有两个种(隐纹水绵,雅托巴水绵)是     

子莲新品种‘武植子莲1号’和‘武植子莲2号’产量与营养品质分析

对子莲（Nelumbo nucifera Gaertn.）新品种‘武植子莲1号’和‘武植子莲2号’与其他12个主栽子莲品种的莲子产量和品质性状进行分析,并通过隶属函数分析法对他们的营养品质性状进行综合评价。结果显示：不同子莲品种的产量和营养品质性状差异显著,同一品种在不同发育时期莲子的可溶性糖和淀粉含量等营养指标差异较大;鲜莲子的可溶性糖含量显著高于成熟莲子,而蛋白质和淀粉含量显著低于成熟莲子。与12个主栽子莲品种相比,‘武植子莲1号’在产量上较为突出,成熟莲子的淀粉含量达52.15%,是生产天然淀粉的优良品种;‘武植子莲2号’鲜莲子的直链淀粉和支链淀粉含量低,可溶性糖含量达23.43%,适合鲜食。隶属函数分析结果表明,‘武植子莲1号’和‘武植子莲2号’的综合营养品质较好,具有很高的应用价值。     

地方性克汀病遗传方式的分析

本文通过1794人、176家系,340户的调查,检出地方性克汀病患者64例,应用分离分析和多基因阈值理论,证实本症属多基因遗传,其加权平均遗传率和标准误为78.51±7.53.     

1,25-双羟胆钙化醇及其受体含量检测的实验研究

 建立了一种改良的血清1,25-双羟胆钙化醇(1,25-Dihydroxycholecalciferol,DHCC)超微量放射受体检测(RRA)技术。完成了灵敏度、精密度、准确度、稳定性及特异性等技术指标。报告了我国健康青年血清DHCC正常值;检测了先天性佝偻病、青春期佝偻病病人及患肾性骨病奶牛等血清DHCC水平。 根据配体与受体相互结合的定量关系,建立了DHCCR(DHCC受体)检测技术。在游离与结合配基分离方面,除建立与比较了DCC(葡聚糖包埋的活性炭)及HAP(羟基磷灰石)方法外,还首次将IEF(等电聚焦电泳)应用于DHCCR分离技术。对佝偻病鸡小肠粘膜上皮细胞受体含量进行了检测并比较了鸡小肠、输卵管壳腺及肝组织DHCCR含量。     

Identification of the C2-1H histidine NMR resonances in chloramphenicol acetyltransferase by a 13C-1H heteronuclear multiple quantum coherence method

Chloramphenicol acetyltransferase (CAT) was used to assess the feasibility of study of specific proton resonances in an enzyme of overall molecular mass 75,000, [ring 2-13C]Histidine was selectively incorporated into the type III chloramphenicol acetyltransferase (CATIII) using a histidine auxotroph of E. coli. Heteronuclear multiple and single quantum experiments were used to select the C2 protons in the histidyl imidazole ring. One- and two-dimensional spectra revealed six signals out of a total of seven histidine residues in CATIII. pH titration, chemical modification and ligand binding were used to demonstrate that the signal from H195, the histidine at the active site, is not among those observed. Nevertheless, this work demonstrates that selective isotopic enrichment and multiple quantum coherence techniques can be used to distinguish proton resonances in a protein of high molecular mass.