全文获取类型
收费全文 | 1460篇 |
免费 | 42篇 |
出版年
2023年 | 25篇 |
2022年 | 28篇 |
2021年 | 47篇 |
2020年 | 32篇 |
2019年 | 48篇 |
2018年 | 39篇 |
2017年 | 42篇 |
2016年 | 48篇 |
2015年 | 48篇 |
2014年 | 96篇 |
2013年 | 106篇 |
2012年 | 110篇 |
2011年 | 147篇 |
2010年 | 87篇 |
2009年 | 67篇 |
2008年 | 85篇 |
2007年 | 82篇 |
2006年 | 80篇 |
2005年 | 70篇 |
2004年 | 66篇 |
2003年 | 55篇 |
2002年 | 45篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1986年 | 3篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1964年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有1502条查询结果,搜索用时 36 毫秒
1.
Kathryn L. Butler Vijayakrishnan Ambravaneswaran Nitin Agrawal Maryelizabeth Bilodeau Mehmet Toner Ronald G. Tompkins Shawn Fagan Daniel Irimia 《PloS one》2010,5(7)
Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility and patient mortality might exist, so far, cumbersome assays have prohibited exploration of the prognostic and diagnostic significance of chemotaxis after burn injury. To address this need, we developed a microfluidic device that is simple to operate and allows for precise and robust measurements of chemotaxis speed and persistence characteristics at single-cell resolution. Using this assay, we established a reference set of migration speed values for neutrophils from healthy subjects. Comparisons with samples from burn patients revealed impaired directional migration speed starting as early as 24 hours after burn injury, reaching a minimum at 72–120 hours, correlated to the size of the burn injury and potentially serving as an early indicator for concurrent infections. Further characterization of neutrophil chemotaxis using this new assay may have important diagnostic implications not only for burn patients but also for patients afflicted by other diseases that compromise neutrophil functions. 相似文献
2.
Fatih M Simsek Bulent Alten Selim S Caglar Yusuf Ozbel A Murat Aytekin Sinan Kaynas Asli Belen Ozge Erisoz Kasap Mehmet Yaman Samiye Rastgeldi 《Journal of vector ecology》2007,32(2):269-279
The two Old World genera, Phlebotomus and Sergentomyia, were both recorded in southern Anatolia in Turkey. Phlebotomus species predominated and comprised about 93% of the entire collection (3,172 specimens). Out of the sixteen species identified, two belonged to the genus Sergentomyia: S. dentata and S. theodori. The remaining fourteen species in the genus Phlebotomus were grouped under four subgenera including some species that are elsewhere known to act as vectors of human cutaneous leishmaniasis. Most of the Phlebotomus were P. tobbi (32.5%), but P. papatasi, P. transcaucasicus, P. halepensis, P. galilaeus, P. sergenti, P. syriacus, P. neglectus, P. simici, P. alexandri, P. similis, P jacusieli, P. perfiliewi, and P. brevis were also identified. There were two associations of sand fly fauna with altitudinal gradient; the first one at relatively higher altitudes and the second one at lower altitudes. The transition between these two assemblages was within the range of 800-1,000 m. It is likely that Adana and Hatay provinces are transitional areas between western and eastern Anatolia. Mountains do not appear to be important geographical barriers for sand fly distribution. We also found that the proven vector P. sergenti is a widely distributed species throughout southern Anatolia and this species, together with its closely related species P. similis, shows sympatry in Konya Province. 相似文献
3.
Mehmet Yaar can 《American journal of physical anthropology》1989,78(1):3-8
This study analyzes and compares the dental size variation and sexual dimorphism of the Highland Beach Mound people, a late southeastern prehistoric aboriginal Florida population (ca. 800-1200 AD) with a somewhat earlier midwestern group (ca. 200-800 AD) and an Archaic sample (ca. 4160-2558 BC). The lengths and breadths of maxillary and mandibular permanent teeth of 99 individuals were measured, and crown surface areas were calculated for the molars. Results indicated that there was little sexual dimorphism. Only the mesiodistal dimensions of the upper P2 and lower C were significantly different at the P less than 0.05 level. In terms of dental size, what appears to be a chronologically based reduction in most points of comparison was seen in all three groups, with the exception of the molars, which were larger in the Highland Beach sample than in the earlier Schultz Mound. 相似文献
4.
Mehmet Rafet Gazvani 《BMJ (Clinical research ed.)》1994,308(6942):1511-1512
5.
Sevinc Yanar Murat Kasap Aylin Kanli Gurler Akpinar Mehmet Sarihan 《Journal of biochemical and molecular toxicology》2023,37(4):e23289
Small cell lung carcinoma (SCLC) is a highly aggressive cancer with low survival rate. Although initial response to chemotherapy in SCLC patients is well-rated, the treatments applied after the disease relapses are not successful. Drug resistance is accepted to be one of the main reasons for this failure. Therefore, there is an urgent need for new treatment strategies for SCLC. Meclofenamic acid, a nonsteroidal anti-inflammatory drug, has been shown to have anticancer effects on various types of cancers via different mechanisms. The aim of this study was to investigate the alterations that meclofenamic acid caused on a SCLC cell line, DMS114 using the tools of proteomics namely two-dimensional gel electrophoresis coupled to MALDI-TOF/TOF and nHPLC coupled to LC-MS/MS. Among the proteins identified by both methods, those showing significantly altered expression levels were evaluated using bioinformatics databases, PANTHER and STRING. The key altered metabolism upon meclofenamic acid treatment appeared to the cellular energy metabolism. Glycolysis was suppressed, whereas mitochondrial activity and oxidative phosphorylation were boosted. The cells underwent metabolic reprogramming to adapt into their new environment for survival. Metabolic reprogramming is known to cause drug resistance in several cancer types including SCLC. The identified differentially regulated proteins in here associated with energy metabolism hold value as the potential targets to overcome drug resistance in SCLC treatment. 相似文献
6.
İncili Çınar Yiğit Arslan Büşra Çelik Esra Nurten Yer Ulu Ferhat Horuz Erdoğan Baloglu Mehmet Cengiz Çağlıyan Ebrar Burcu Gamze Bayarslan Aslı Ugurlu Altunoglu Yasemin Celik 《Protoplasma》2023,260(2):509-527
Protoplasma - Watermelon and melon are members of the Cucurbitaceae family including economically significant crops in the world. The expansin protein family, which is one of the members of the... 相似文献
7.
Prof. Dr. Mehmet Emin Duru Büşra Eroğlu Assoc. Prof. Dr. Gülsen Tel-Çayan Meltem Taş-Küçükaydın Dr. Selçuk Küçükaydın Dr. Fatih Çayan Prof. Dr. Özgür Ceylan 《化学与生物多样性》2023,20(6):e202300486
Sunflower honey (SH) is bright yellow, fragrant, pollen-flavoured, slightly herbaceous and has a unique taste. The present research aims to examine the enzyme inhibitory, antioxidant, anti-inflammatory, antimicrobial and anti-quorum sensing activities and phenolic compositions of 30 sunflower honeys (SHs) produced from several regions of Turkey with chemometric study. SAH from Samsun exhibited the best antioxidant activity in β-carotene linoleic acid (IC50: 7.33±0.17 mg/mL) and CUPRAC (A0.50: 4.94±0.13 mg/mL) assays, anti-urease activity (60.63±0.87 %) and anti-inflammatory activity against COX-1 (73.94±1.08 %) and COX-2 (44.96±0.85 %). SHs exhibited mild antimicrobial activity against the test microorganisms while they showed high quorum sensing inhibition zones measured in the range of 42–52 mm against the CV026 strain. The phenolic composition was determined by high performance liquid chromatography with diode array detection (HPLC-DAD) system and levulinic, gallic, p-hydroxybenzoic, vanillic and p-coumaric acids were identified in all studied SHs. The classification of SHs was performed the using PCA and HCA. This study revealed that phenolic compounds and biological properties are effective in classification of SHs according to their geographical origin. The results suggest that studied SHs could be valued as potential agents with versatile bioactivities in oxidative stress-related disease, microbial infections, inflammation, melanoma, and peptic ulcer. 相似文献
8.
Samet Evyapan Emine Elçin Oruç-Emre Yusuf Sıcak Ayşegül Karaküçük-İyidoğan Gizem Tatar Yılmaz Mehmet Öztürk 《化学与生物多样性》2023,20(8):e202300626
In this study, new chiral thiourea and 1,3-thiazolidine-4,5-dione derivatives were synthesized, it was aimed to evaluate the various biological activities and molecular docking of these compounds. Firstly, the new thioureas ( 1 – 16 ) were obtained by reacting 1-naphthylisothiocyanate with different chiral amines. Then, the chiral thioureas were cyclized with oxalyl chloride to obtain 1,3-thiazolidine-4,5-dione derivatives ( 17 – 32 ). All compounds were evaluated with several in vitro antioxidant and enzyme inhibition activities. Compound 30 was the most active compound against AChE, with a value of IC50=8.09±0.58 μM. On the other hand, all compounds were tested in silico absorption, distribution, metabolism, and excretion (ADME) assays to better understand their bioavailability. These physicochemical properties, pharmacokinetics, and drug-likeness of all compounds were calculated using SwissADME. Furthermore, according to molecular docking analyses compound 30 exhibited significant binding affinities for all enzymes. Based on our overall observations, compound 30 could be recommended as a potential lead for the therapuetic of Alzheimer's. 相似文献
9.
Peter Malessa Klaus H. Rockenfeller Mehmet A. Pak Phillip-P. Roth 《Biological cybernetics》1982,43(1):29-34
By considering the frequency dependence of the ocular volume elasticity it is possible to locate the static volume elasticity function of the avascular compartment of the eye in vivo. The procedure used involved measuring the dynamic volume elasticity function E=f(P, v), where E=volume elasticity, P=intraocular pressure, and v=frequency, in vivo and post mortem at a frequency higher than the apparent upper mechanical response frequency of the intraocular vascular bed. In addition, post mortem measurements were made of the volume elasticity function at a frequency which was as low as experimentally possible. For practical purposes the latter volume elasticity function may serve as an estimate of the static elasticity function of the avascular compartment in vivo. This is possible in all cases because at the high frequency level the dynamic volume elasticity functions measured in vivo and post mortem are identical.Partly presented by the first author at the 4th Mackenzie Symposium, Stirling 1977Decaased 18.3. 1980 相似文献
10.
Revealing organizational principles of biological networks is an important goal of systems biology. In this study, we sought to analyze the dynamic organizational principles within the protein interaction network by studying the characteristics of individual neighborhoods of proteins within the network based on their gene expression as well as protein-protein interaction patterns. By clustering proteins into distinct groups based on their neighborhood gene expression characteristics, we identify several significant trends in the dynamic organization of the protein interaction network. We show that proteins with distinct neighborhood gene expression characteristics are positioned in specific localities in the protein interaction network thereby playing specific roles in the dynamic network connectivity. Remarkably, our analysis reveals a neighborhood characteristic that corresponds to the most centrally located group of proteins within the network. Further, we show that the connectivity pattern displayed by this group is consistent with the notion of “rich club connectivity” in complex networks. Importantly, our findings are largely reproducible in networks constructed using independent and different datasets. 相似文献