排序方式: 共有94条查询结果,搜索用时 15 毫秒
91.
P. J. Eswari Pandaranayaka J. Kanagavalli S. R. Krishnadas P. Sundaresan S. Krishnaswamy 《World journal of microbiology & biotechnology》2008,24(6):903-907
Human myocilin is a 55 kDa protein that is implicated in primary open angle glaucoma (POAG). Understanding the structure and
folding of the native protein and the mutants that increase aggregation could lead to possible prevention of the condition.
We report here the over expression and purification of the human myocilin in E. coli. The initial expression of recombinant myocilin in E. coli was found to be low. The problem of low yield was found to be due to multiple causes and was overcome using a suitable combination
of vectors, tags, host background and expression protocols. The overexpressed human myocilin was purified by affinity column
chromatography to yield about 8 mg of protein from 1 l of culture. The protein purity and folding were confirmed using electrophoresis,
immunoblotting and fluorescence spectroscopy. Further biophysical characterization and crystallization trials using the recombinant
human myocilin will pave the way for better understanding of the structure–aggregation relationship that is involved in causing
POAG. 相似文献
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Lina J. Bird Rebecca L. Mickol Brian J. Eddie Meghna Thakur Matthew D. Yates Sarah M. Glaven 《Microbial biotechnology》2023,16(3):494-506
The junction of bioelectrochemical systems and synthetic biology opens the door to many potentially groundbreaking technologies. When developing these possibilities, choosing the correct chassis organism can save a great deal of engineering effort and, indeed, can mean the difference between success and failure. Choosing the correct chassis for a specific application requires a knowledge of the metabolic potential of the candidate organisms, as well as a clear delineation of the traits, required in the application. In this review, we will explore the metabolic and electrochemical potential of a single genus, Marinobacter. We will cover its strengths, (salt tolerance, biofilm formation and electrochemical potential) and weaknesses (insufficient characterization of many strains and a less developed toolbox for genetic manipulation) in potential synthetic electromicrobiology applications. In doing so, we will provide a roadmap for choosing a chassis organism for bioelectrochemical systems. 相似文献
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Pierre Gillotay Meghna Shankar Benoit Haerlingen Eski Sema Elif Macarena PozoMorales Ins Garteizgogeascoa Susanne Reinhardt Annekathrin Krnkel Juliane Blsche Andreas Petzold Nikolay Ninov Gokul Kesavan Christian Lange Michael Brand Anne Lefort Frdrick Libert Vincent Detours Sabine Costagliola Singh Sumeet Pal 《EMBO reports》2020,21(12)