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121.
Commentary: Carbon Metabolism of the Terrestrial Biosphere: A Multitechnique Approach for Improved Understanding 总被引:2,自引:0,他引:2
J. G. Canadell H. A. Mooney D. D. Baldocchi J. A. Berry J. R. Ehleringer C. B. Field S. T. Gower D. Y. Hollinger J. E. Hunt R. B. Jackson S. W. Running G. R. Shaver W. Steffen S. E. Trumbore R. Valentini B. Y. Bond 《Ecosystems》2000,3(2):115-130
Understanding terrestrial carbon metabolism is critical because terrestrial ecosystems play a major role in the global carbon
cycle. Furthermore, humans have severely disrupted the carbon cycle in ways that will alter the climate system and directly
affect terrestrial metabolism. Changes in terrestrial metabolism may well be as important an indicator of global change as
the changing temperature signal. Improving our understanding of the carbon cycle at various spatial and temporal scales will
require the integration of multiple, complementary and independent methods that are used by different research communities.
Tools such as air sampling networks, inverse numerical methods, and satellite data (top-down approaches) allow us to study
the strength and location of the global- and continental-scale carbon sources and sinks. Bottom-up studies provide estimates
of carbon fluxes at finer spatial scales and examine the mechanisms that control fluxes at the ecosystem, landscape, and regional
scales. Bottom-up approaches include comparative and process studies (for example, ecosystem manipulative experiments) that
provide the necessary mechanistic information to develop and validate terrestrial biospheric models. An iteration and reiteration
of top-down and bottom-up approaches will be necessary to help constrain measurements at various scales. We propose a major
international effort to coordinate and lead research programs of global scope of the carbon cycle.
Received 7 May 1999; accepted 28 September 1999. 相似文献
122.
Lineage-specific evolution and gene flow in Listeria monocytogenes are independent of bacteriophages
Roxana Zamudio Richard D. Haigh Joseph D. Ralph Megan De Ste Croix Taurai Tasara Katrin Zurfluh Min Jung Kwun Andrew D. Millard Stephen D. Bentley Nicholas J. Croucher Roger Stephan Marco R. Oggioni 《Environmental microbiology》2020,22(12):5058-5072
Listeria monocytogenes is a foodborne pathogen causing systemic infection with high mortality. To allow efficient tracking of outbreaks a clear definition of the genomic signature of a cluster of related isolates is required, but lineage-specific characteristics call for a more detailed understanding of evolution. In our work, we used core genome MLST (cgMLST) to identify new outbreaks combined to core genome SNP analysis to characterize the population structure and gene flow between lineages. Whilst analysing differences between the four lineages of L. monocytogenes we have detected differences in the recombination rate, and interestingly also divergence in the SNP differences between sub-lineages. In addition, the exchange of core genome variation between the lineages exhibited a distinct pattern, with lineage III being the best donor for horizontal gene transfer. Whilst attempting to link bacteriophage-mediated transduction to observed gene transfer, we found an inverse correlation between phage presence in a lineage and the extent of recombination. Irrespective of the profound differences in recombination rates observed between sub-lineages and lineages, we found that the previously proposed cut-off of 10 allelic differences in cgMLST can be still considered valid for the definition of a foodborne outbreak cluster of L. monocytogenes. 相似文献
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Like all parasitic protozoa, the human malaria parasite Plasmodium falciparum lacks the enzymes required for de novo synthesis of purines and it is therefore reliant upon the salvage of these compounds from the external environment. P. falciparum equilibrative nucleoside transporter 1 (PfENT1) is a nucleoside transporter that has been localized to the plasma membrane of the intraerythrocytic form of the parasite. In this study we have characterized the transport of purine and pyrimidine nucleosides across the plasma membrane of 'isolated' trophozoite-stage P. falciparum parasites and compared the transport characteristics of the parasite with those of PfENT1 expressed in Xenopus oocytes. The transport of nucleosides into the parasite: (i) was, in the case of adenosine, inosine and thymidine, very fast, equilibrating within a few seconds; (ii) was of low affinity [K(m) (adenosine) = 1.45 +/- 0.25 mM; K(m) (thymidine) = 1.11 +/- 0.09 mM]; and (iii) showed 'cross-competition' for adenosine, inosine and thymidine, but not cytidine. The kinetic characteristics of nucleoside transport in intact parasites matched very closely those of PfENT1 expressed in Xenopus oocytes [K(m) (adenosine) = 1.86 +/- 0.28 mM; K(m) (thymidine) = 1.33 +/- 0.17 mM]. Furthermore, PfENT1 transported adenosine, inosine and thymidine, with a cross-competition profile the same as that seen for isolated parasites. The data are consistent with PfENT1 serving as a major route for the uptake of nucleosides across the parasite plasma membrane. 相似文献
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We examined the anaerobic degradation of phenol and the ortho, meta, and para isomers of chlorophenol, methoxyphenol, methylphenol (cresol), and nitrophenol in anaerobic sewage sludge diluted to 10% in a mineral salts medium. Of the 12 monosubstituted phenols studied, only p-chlorophenol and o-cresol were not significantly degraded during an 8-week incubation period. The phenol compounds degraded and the time required for complete substrate disappearance (in weeks) were: phenol (2), o-chlorophenol (3), m-chlorophenol (7), o-methoxyphenol (2), m- and p-methoxyphenol (1), m-cresol (7), p-cresol (3), and o-, m-, and p-nitrophenol (1). Complete mineralization of phenol, o-chlorophenol, m-cresol, p-cresol, o-nitrophenol, p-nitrophenol, and o-, m-, and p-methoxyphenol was observed. In general, the presence of Cl and NO2 groups on phenols inhibited methane production. Elimination or transformation of these substituents was accompanied by increased methane production, o-Chlorophenol was metabolized to phenol, which indicated that dechlorination was the initial degradation step. The methoxyphenols were transformed to the corresponding dihydroxybenzene compounds, which were subsequently mineralized. 相似文献
129.
P. S. Blair P. J. Fleming D. Bensley I. Smith C. Bacon E. Taylor J. Berry J. Golding J. Tripp 《BMJ (Clinical research ed.)》1996,313(7051):195-198
OBJECTIVE--To investigate the effects of exposure to tobacco smoke and of parental consumption of alcohol and illegal drugs as risk factors for the sudden infant death syndrome after a national risk reduction campaign which included advice on prenatal and postnatal avoidance of tobacco smoke. DESIGN--Two year population based case-control study. Parental interviews were conducted for each infant who died and four controls matched for age and date of interview. SETTING--Three regions in England with a total population of 17 million people. SUBJECTS--195 babies who died and 780 matched controls. RESULTS--More index than control mothers (62.6% v 25.1%) smoked during pregnancy (multivariate odds ratio = 2.10; 95% confidence interval 1.24 to 3.54). Paternal smoking had an additional independent effect when other factors were controlled for (2.50; 1.48 to 4.22). The risk of death rose with increasing postnatal exposure to tobacco smoke, which had an additive effect among those also exposed to maternal smoking during pregnancy (2.93; 1.56 to 5.48). The population attributable risk was over 61%, which implies that the numbers of deaths from the syndrome could be reduced by almost two third if parents did not smoke. Alcohol use was higher among index than control mothers but was strongly correlated with smoking and on multivariate analysis was not found to have any additional independent effect. Illegal drug use was more common among the index parents, and paternal use of illegal drugs remained significant in the multivariate model (4.68; 1.56 to 14.05). CONCLUSIONS--This study confirms the increased risk of the sudden infant death syndrome associated with maternal smoking during pregnancy and shows evidence that household exposure to tobacco smoke has an independent additive effect. Parental drug misuse has an additional small but significant effect. 相似文献
130.
Ecological surveys were carried out to investigate the distribution and characterization of remaining mangrove stands in Hong
Kong. The field studies indicate that 43 mangrove stands, excluding Mai Po Nature Reserve, still remained along the coastline
of Hong Kong despite tremendous reclamation and development which occurred in the past 40 years. Most mangrove stands were
found in Deep Bay (western part)and Sai Kung District (eastern coasts). The total areas occupied by these mangrove stands
were 178 ha,varying from a very small stand (with 1–2 mangrove shrubs) to fairly extensive mangroves in Deep Bay (> 10 ha).
It appeared that mangrove stands located in Deep Bay area were larger than those in the eastern coasts. Twenty plant species
were identified from these stands, with 13 being exclusive or associate mangrove species. The major constituent species were
Kandelia candel, Aegiceras corniculatum, Excoecaria agallocha and Avicennia marina. Rare species such as Heritiera littoralis
were only found in a few mangrove stands. Out of the 43remaining mangrove stands, 23 were more worthwhile for conservation
and their plant community structures were further investigated by transect and quadrat analyses. The importance values (sum
of relative abundance,frequency and dominance) show that K. candel was the most dominant species. Species richness and Simpson's
indices together with tree height, tree density and canopy area fluctuated significantly between mangrove stands. These values
were used to prioritize the conservation potential of the remaining mangrove stands in Hong Kong.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献