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Mixtures of unsaturated lipids, sphingolipids, and cholesterol form coexisting liquid-disordered and sphingolipid and cholesterol-rich liquid-ordered (Lo) phases in water. The detergent Triton X-100 does not readily solubilize Lo domains, but does solubilize liquid-disordered domains, and is commonly used to prepare detergent-resistant membranes from cells and model membranes. However, it has been proposed that in membranes with mixtures of sphingomyelin (SM), 1-palmitoyl 2-oleoyl phosphatidylcholine (POPC), and cholesterol Triton X-100 may induce Lo domain formation, and therefore detergent-resistant membranes may not reflect the presence of preexisting domains. To examine this hypothesis, the effect of Triton on Lo domain formation was measured in SM/POPC/cholesterol vesicles. Nitroxide quenching methods that can detect ordered nanodomains with radii >12 Å showed that in the absence of Triton X-100 this mixture formed ordered state domains that melt with a midpoint (= Tmid) at ∼45°C. However, Tmid was lower when detected using various fluorescence resonance energy transfer (FRET) pairs. Furthermore, the Tmid value was Ro dependent, and decreased as Ro increased. Because FRET can only readily detect domains with radii >Ro, this result can be explained by domain radii that are close to Ro and decrease as temperature increases. An analysis of FRET and quenching data suggests that nanodomain radius gradually decreases from ≥150 Å to <40 Å as temperature increases from 10 to 45°C. Interestingly, the presence of Triton X-100 or a transmembrane-type peptide did not stabilize ordered state formation when detected by nitroxide quenching, i.e., did not increase Tmid. However, FRET-detected Tmid did increase in the presence of Triton X-100 or a transmembrane peptide, indicating that both increased domain size. Controls showed that the results could not be accounted for by probe-induced perturbations. Thus, SM/POPC/cholesterol, a mixture similar to that in the outer leaflet of plasma membranes, forms nanodomains at physiological temperatures, and TX-100 does not induce domain formation or increase the fraction of the bilayer in the ordered state, although it does increase domain size by coalescing preexisting domains. 相似文献
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Tarala D. Nandedkar Medha S. Rajadhyaksha Rita R. Mukhopadhya S. G. A. Rao D. S. Joshf 《Journal of biosciences》1998,23(3):271-277
Ovarian follicular fluid peptide (OFFP) purified from sheep ovaries has been earlier shown to induce degeneration of ovarian
follicles in mice. In the present study, whether the effect of OFFP on granulosa cells was similar to apoptosis was studied
using three parameters. Immature mice injected with pregnant mare serum gonadotropin on day 0 were administered with 10 or
20 μg of OFFP on day 1 and autopsied on day 2. The granulosa cells were collected from the ovarian follicles. The presence
of apoptotic bodies were observed by staining the cells with acridine orange. DNA profiles of DAPI-stained cells analysed
by flow cytometry also revealed apoptotic response to OFFP. Furthermore, agarose gel electrophoresis of low molecular weight
DNA fraction extracted from the cells of OFFP-treated animals confirmed ladder formation and induction of apoptosis and not
necrosis in granulosa cells. In conclusion, all the three parameters indicated apoptotic changes in granulosa cells of ovarian
follicles in .mice treated with OFFP. The effect of OFFP seems to be exerted directly on the granulosa cells showing its autocrine
role in the process of follicular atresia. This is discussed in the light of other intra/extra ovarian factors. 相似文献
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Benjamin A. Pinsky Malaya K. Sahoo Johanna Sandlund Marika Kleman Medha Kulkarni Per Grufman Malin Nygren Robert Kwiatkowski Ellen Jo Baron Fred Tenover Blake Denison Russell Higuchi Reuel Van Atta Neil Reginald Beer Alda Celena Carrillo Pejman Naraghi-Arani Chad E. Mire Charlene Ranadheera Allen Grolla Nina Lagerqvist David H. Persing 《PloS one》2015,10(11)
BackgroundThe recently developed Xpert® Ebola Assay is a novel nucleic acid amplification test for simplified detection of Ebola virus (EBOV) in whole blood and buccal swab samples. The assay targets sequences in two EBOV genes, lowering the risk for new variants to escape detection in the test. The objective of this report is to present analytical characteristics of the Xpert® Ebola Assay on whole blood samples.ConclusionIn summary, we found the Xpert® Ebola Assay to have high analytical sensitivity and specificity for the detection of EBOV in whole blood. It offers ease of use, fast turnaround time, and remote monitoring. The test has an efficient viral inactivation protocol, fulfills inclusivity and exclusivity criteria, and has specimen stability characteristics consistent with the need for decentralized testing. The simplicity of the assay should enable testing in a wide variety of laboratory settings, including remote laboratories that are not capable of performing highly complex nucleic acid amplification tests, and during outbreaks where time to detection is critical. 相似文献
85.
Puri Vikram Priyadarshini Ishaani Kumar Raghvendra Van Le Chung 《Cluster computing》2021,24(3):1675-1694
Cluster Computing - Internet of Things (IoT) is one of the most powerful platforms that incorporates several other technological components within itself. The IoT ecosystem comprises devices,... 相似文献
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Jyoti Mareddy Suresh Babu Nallapati Jayasree Anireddy Yumnam Priyadarshini Devi Lakshmi Narasu Mangamoori Ravikumar Kapavarapu Sarbani Pal 《Bioorganic & medicinal chemistry letters》2013,23(24):6721-6727
A new class of 1,2,3-triazol derivatives derived from nimesulide was designed as potential inhibitors of PDE4B. Synthesis of these compounds was carried out via a multi-step sequence consisting of copper-catalyzed azide–alkyne cycloaddition (CuAAC) as a key step in aqueous media. The required azide was prepared via the reaction of aryl amine (obtained from nimesulide) with α-chloroacetyl chloride followed by displacing the α-chloro group by an azide. Some of the synthesized compounds showed encouraging PDE4B inhibitory properties in vitro that is >50% inhibition at 30 μM that were supported by the docking studies of these compounds at the active site of PDE4B enzyme (dock scores ~ ?28.6 for a representative compound). Two of these PDE4 inhibitors showed promising cytotoxic properties against HCT-15 human colon cancer cells in vitro with IC50 ~ 21–22 μg/mL. 相似文献
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Molecular Analysis of Drought Tolerance in Tea by cDNA-AFLP Based Transcript Profiling 总被引:1,自引:0,他引:1
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Tripti Dogra Anupama Priyadarshini Kanika Ashok Kumar Nagendra Kumar Singh 《Symbiosis (Philadelphia, Pa.)》2013,61(3):135-143
In an attempt to find the genes involved in salt tolerance of the highly adaptable chickpea rhizobium strain, Mesorhizobium ciceri Ca181, a Tn5 transposon insertion library was generated and screened to identify five mutants with inability to survive in the presence of 0.1 M NaCl. The genes disrupted in these mutants due to insertion of the transposon were identified by sequencing of Tn5 flanking sequences after inverse PCR. One of the mutants had a disruption in diguanylate cyclase gene which is involved in bacterial biofilm formation and persistence. The second mutant had a disruption in an ABC transporter membrane protein gene, which is involved in the uptake of nutrients and cellular osmoprotection. The third mutant had a disruption in a gene showing homology with rhamnulose 1-phosphate aldolase which has an important role in the central metabolism of L-rhamnulose. The fourth mutant had a disruption in a capsule synthesis gene and the fifth mutant had an insertion in an oxidoreductase gene. When these mutants were inoculated into the host chickpea plant under normal non-saline conditions, they formed symbiotic nodules but with severely reduced nitrogenase activity. Hence, it appears that bacterial ability to adapt to hyper-osmotic salt stress conditions is also important for its nitrogen fixing ability in the chickpea root nodules. Allele mining for variant forms of the identified genes in the germplasm resources of M. ciceri may help in the development of highly adaptive and efficient nitrogen fixing strains of the chickpea rhizobium. 相似文献
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Saradadevi Gargi Prasad Priyadarshini Neha Bera Aveepsha Mohannath Gireesha 《Journal of plant biochemistry and biotechnology.》2020,29(4):743-753
Journal of Plant Biochemistry and Biotechnology - Eukaryotic cells carry several hundreds of ribosomal RNA (rRNA) genes which are clustered as tandem repeats at chromosomal loci called nucleolus... 相似文献
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Harikishore Pingali Mukul Jain Shailesh Shah Pandurang Zaware Pankaj Makadia Suresh Pola Baban Thube Darshit Patel Pravin Patil Priyanka Priyadarshini Dinesh Suthar Maanan Shah Suresh Giri Pankaj Patel 《Bioorganic & medicinal chemistry letters》2010,20(3):1156-1161
Bis-oximinoalkanoic acid derivatives were designed and synthesized to aid in the characterization of selective PPARα agonists by replacing the oxazole ring with flexible oximino group in the lipophilic tail part of a previously reported compound 3. Selected compounds 9d and 9m showed excellent potency and high selectivity towards PPARα in vitro. These compounds found effective in reducing serum triglycerides (TG) in vivo. 相似文献