全文获取类型
收费全文 | 1519篇 |
免费 | 82篇 |
国内免费 | 2篇 |
专业分类
1603篇 |
出版年
2024年 | 4篇 |
2023年 | 18篇 |
2022年 | 42篇 |
2021年 | 73篇 |
2020年 | 37篇 |
2019年 | 40篇 |
2018年 | 75篇 |
2017年 | 47篇 |
2016年 | 58篇 |
2015年 | 76篇 |
2014年 | 100篇 |
2013年 | 90篇 |
2012年 | 101篇 |
2011年 | 75篇 |
2010年 | 52篇 |
2009年 | 54篇 |
2008年 | 77篇 |
2007年 | 78篇 |
2006年 | 55篇 |
2005年 | 50篇 |
2004年 | 58篇 |
2003年 | 43篇 |
2002年 | 29篇 |
2001年 | 23篇 |
2000年 | 32篇 |
1999年 | 17篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 10篇 |
1995年 | 7篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 16篇 |
1991年 | 15篇 |
1990年 | 16篇 |
1989年 | 18篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 10篇 |
1985年 | 7篇 |
1984年 | 13篇 |
1983年 | 6篇 |
1981年 | 9篇 |
1980年 | 7篇 |
1977年 | 4篇 |
1975年 | 5篇 |
1974年 | 2篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1965年 | 2篇 |
排序方式: 共有1603条查询结果,搜索用时 0 毫秒
41.
42.
43.
Md. Aktar-Uz-Zaman Md. Ariful Islam Md. Shahin Iqbal Md. Jahangir Alam Debashish Sarkar Bander Albogami Ahmed Gaber Akbar Hossain 《Phyton》2023,92(3):883-899
Ensuring food security for the rapidly increasing population and changing climatic scenarios are requisites for
exploiting the genetic divergence of food crops. A study was undertaken to sort out an early maturing chickpea
variety for fitting easily between rice-rice cropping systems in the Eastern Indo-Gangetic Plain of Bangladesh. The
trial was comprised of eight elite lines of chickpea and executed at various localities in Bangladesh from 2014–
15 to 2017–18. The result explored the chickpea genotype, BARI Chola-11 remained superior to the rest of
the elite genotypes for having a short maturity period (100–106 days), and lesser days to 50% flowering (47–
55 days). The same genotype was recorded to have robust vegetative and reproductive yield attributes including
plant height (49–57 cm), podsplant−1 (37–50), and optimum 100 seed weight (19.5–20.6 g). Owing to better yield
attributes, BARI Chola-11 resulted in the maximum seed yield (1200–1500 kg ha−1
) of chickpea and might be
recommended for general adoption in the region for boosting nutritional security status through improved productivity under changing climate. 相似文献
44.
45.
Vaibhav K Shrivastava P Javed H Khan A Ahmed ME Tabassum R Khan MM Khuwaja G Islam F Siddiqui MS Safhi MM Islam F 《Molecular and cellular biochemistry》2012,367(1-2):73-84
The pathophysiological mechanisms leading to neuronal injury in middle cerebral artery occlusion (MCAO) model of cerebral stroke are complex and multifactorial that form the bases of behavioral deficits and inflammation mediated damage. The present study demonstrates the effect of piperine pretreatment (10 mg/kg b wt, once daily p.o. for 15 days) on cerebral ischemia-induced inflammation in male Wistar rats. The right middle cerebral artery was occluded for 2 h followed by reperfusion for 22 h. A maximum infarct volume (57.80 %) was observed in ischemic MCAO group. However, piperine administration prior to ischemia showed a significant reduction in infarct volume (28.29 %; p < 0.05) and neuronal loss (12.72 %; p < 0.01). As a result of piperine pretreatment, a significant improvement in behavioral outputs of MCAO rats (p < 0.05-0.01) was observed. Piperine successfully reduced the level of proinflammatory cytokines IL-1β, IL-6 and TNF-α, in ischemic group (p < 0.01). Ischemic group brain has shown edematous morphology with vacuolated architecture and pyknotic nuclei in H & E staining which was successfully ameliorated by piperine administration. Moreover, piperine also succeeded in lowering the expression of COX-2, NOS-2, and NF-κB (p < 0.01). Both cytosolic and nuclear NF-κB were down-regulated in ischemic group pre-administered with piperine (p < 0.01). The present study suggests that piperine is able to salvage the ischemic penumbral zone neurons by virtue of its anti-inflammatory property, thereby limiting ischemic cell death. 相似文献
46.
Immunoperoxidase detection of myeloid antigens in glycolmethacrylate-embedded human bone marrow 总被引:2,自引:0,他引:2
E Archimbaud A Islam H D Preisler 《The journal of histochemistry and cytochemistry》1987,35(5):595-599
Different methods for fixation and exposure of antigenic determinants were tested for detection of a granulocytic differentiation antigen by the monoclonal antibody L12-2, using an indirect immunoperoxidase method on semi-thin sections of undecalcified, glycolmethacrylate-embedded human bone marrow biopsies. Fixation in Bouin's solution for 3 hr gave a more intense and more homogeneous immunological staining than fixation in absolute methanol, 4% formalin, B5, or Michel's medium, and the morphological detail was excellent. Digestion by pronase or trypsin was required. Coating the glass slides with Alcian blue prevented loss of sections from the slides during the staining procedure. Bouin fixation also made possible detection of two other differentiation antigens expressed in the granulocytic series, using the monoclonal antibodies 1G10 and R1B19. Furthermore, the same technique also permitted detection of factor VIII-RAg in the megakaryocytes, as well as recognition of cells of the erythroid series by use of polyclonal rabbit antisera. 相似文献
47.
Sato Nana Yuzawa Mao Aminul Md Islam Tomokiyo Mikado Albarracin Leonardo Garcia-Castillo Valeria Ideka-Ohtsubo Wakako Iwabuchi Noriyuki Xiao Jin-zhong Garcia-Cancino Apolinaria Villena Julio Kitazawa Haruki 《Probiotics and antimicrobial proteins》2021,13(3):824-836
Probiotics and Antimicrobial Proteins - The use of in vitro systems that allow efficient selection of probiotic candidates with immunomodulatory properties could significantly minimize the use of... 相似文献
48.
49.
Protein methyltransferases (PMTs) catalyze arginine and lysine methylation of diverse histone and nonhistone targets. These posttranslational modifications play essential roles in regulating multiple cellular events in an epigenetic manner. In the recent process of defining PMT targets, S-adenosyl-L-methionine (SAM) analogues have emerged as powerful small molecule probes to label and profile PMT targets. To examine efficiently the reactivity of PMTs and their variants on SAM analogues, we transformed a fluorogenic PMT assay into a ready high throughput screening (HTS) format. The reformulated fluorogenic assay is featured by its uncoupled but more robust character with the first step of accumulation of the commonly-shared reaction byproduct S-adenosyl-L-homocysteine (SAH), followed by SAH-hydrolase-mediated fluorogenic quantification. The HTS readiness and robustness of the assay were demonstrated by its excellent Z' values of 0.83-0.95 for the so-far-examined 8 human PMTs with SAM as a cofactor (PRMT1, PRMT3, CARM1, SUV39H2, SET7/9, SET8, G9a and GLP1). The fluorogenic assay was further implemented to screen the PMTs against five SAM analogues (allyl-SAM, propargyl-SAM, (E)-pent-2-en-4-ynyl-SAM (EnYn-SAM), (E)-hex-2-en-5-ynyl-SAM (Hey-SAM) and 4-propargyloxy-but-2-enyl-SAM (Pob-SAM)). Among the examined 8 × 5 pairs of PMTs and SAM analogues, native SUV39H2, G9a and GLP1 showed promiscuous activity on allyl-SAM. In contrast, the bulky SAM analogues, such as EnYn-SAM, Hey-SAM and Pob-SAM, are inert toward the panel of human PMTs. These findings therefore provide the useful structure-activity guidance to further evolve PMTs and SAM analogues for substrate labeling. The current assay format is ready to screen methyltransferase variants on structurally-diverse SAM analogues. 相似文献
50.