全文获取类型
收费全文 | 2117篇 |
免费 | 72篇 |
国内免费 | 6篇 |
专业分类
2195篇 |
出版年
2024年 | 3篇 |
2023年 | 26篇 |
2022年 | 83篇 |
2021年 | 142篇 |
2020年 | 89篇 |
2019年 | 98篇 |
2018年 | 102篇 |
2017年 | 89篇 |
2016年 | 105篇 |
2015年 | 149篇 |
2014年 | 171篇 |
2013年 | 167篇 |
2012年 | 157篇 |
2011年 | 140篇 |
2010年 | 90篇 |
2009年 | 78篇 |
2008年 | 107篇 |
2007年 | 74篇 |
2006年 | 62篇 |
2005年 | 67篇 |
2004年 | 59篇 |
2003年 | 33篇 |
2002年 | 39篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1964年 | 3篇 |
1962年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有2195条查询结果,搜索用时 15 毫秒
851.
Iram Parveen Naseem Ahmed Danish Idrees Parvez Khan Md. Imtaiyaz Hassan 《Bioorganic & medicinal chemistry letters》2017,27(18):4493-4499
Substituted 2-amino-7-((6-(4-(2-hydroxyethyl) piperazin-1-yl)-2-methylpyrimidin-4-yl)oxy)-4-phenyl-4H-chromene-3-carbonitriles and 2-amino-7-((6-(4-(2-hydroxyethyl)piperazin-1-yl)-2-methylpyrimidin-4-yl)oxy)-4-phenyl-1,4-dihydroquinoline-3-carbonitriles were synthesized via an efficient multi-component one pot synthesis under mild conditions. These compounds 1–20 were evaluated against human breast cancer cell lines (MCF-7) and human embryonic kidney cells (HEK293) for cytotoxic activities. Among them, compounds 6, 7, 15, 17 and 19 showed better anti-proliferative activities as (IC50 value 48 ± 1.70, 65 ± 1.13, 92 ± 1.18, 30 ± 1.17 and 16 ± 1.10 µM) than curcumin drug (48 ± 1.11 µM). Molecular docking was also performed with active compounds 6, 7 and 15 against Bcl-2 protein which gave good binding affinity (ΔG = ?9.08, ?8.29 and ?7.70 kcal/mol) respectively. Furthermore, the structure-activity relationship (SAR) analysis revealed that the chromene and quinoline moieties, when attached with pyrimide and piperazine moieties, enhanced anti-proliferative activities. 相似文献
852.
How well documented is Australia's flora? Understanding spatial bias in vouchered plant specimens 下载免费PDF全文
Md. Mohasinul Haque David A. Nipperess Rachael V. Gallagher Linda J. Beaumont 《Austral ecology》2017,42(6):690-699
Massive digitization of natural history collections (NHC) has opened the door for researchers to conduct inferential studies on the collection of biological diversity across space and time. The widespread use of NHCs in scientific research makes it essential to characterize potential sources of spatial bias. In this study, we assessed spatial patterns in records from the Australian Virtual Herbarium (AVH), based on >3 000 000 vouchered specimens of around 21 000 native plant species. The AVH is the main database for describing Australia's flora, and identifying its limitations is of paramount interest for the validity of conservation and environmental studies. We characterized how sampling effort is distributed across each Interim Bioregion of Australia (IBRA), then asked: (i) How complete are species inventories for each bioregion? We define completeness (C) as the ratio of observed to estimated species richness, using the Chao 1 estimator, (ii) How is sampling effort related to a commonly used Human Influence Index (HII)? and (iii) What is the probability that additional collections would result in the identification of previously unrecorded species in each bioregion? Sampling effort across bioregions is unequal, which partially reflects the collecting behaviour of naturalists in relation to species richness patterns. The density of records in bioregions ranges from 0.02–8.37 km?2. At the bioregional scale, completeness is generally high with 79% of bioregions estimated to have records for at least 80% of their species. Completeness is partly explained by sampling effort (r = 0.43, p = 0.01), although some bioregions (e.g. Northern Kimberley and Burt Plain) have high completeness yet relatively low sampling effort. The inventory of Hampton, however, is substantially less complete than other bioregions (C = 0.66). Bioregions with high HII consistently have high completeness, while regions with low HII span the full range of completeness values. We calculated that an additional specimen collected from a bioregion has a 0.33% (Wet Tropics) to 11.7% (Arnhem Coast) probability of representing a new species for that region. Our assessment can assist with directing future systematic survey efforts by identifying bioregions where additional surveying may result in the greatest return, in terms of increasing knowledge of species richness and diversity. 相似文献
853.
Rhamnose synthase activity is required for pathogenicity of the vascular wilt fungus Verticillium dahliae 总被引:1,自引:0,他引:1 下载免费PDF全文
Parthasarathy Santhanam Jordi C. Boshoven Omar Salas Kyle Bowler Md Tohidul Islam Mojtaba Keykha Saber Grardy C. M. van den Berg Maor Bar‐Peled Bart P. H. J. Thomma 《Molecular Plant Pathology》2017,18(3):347-362
The initial interaction of a pathogenic fungus with its host is complex and involves numerous metabolic pathways and regulatory proteins. Considerable attention has been devoted to proteins that play a crucial role in these interactions, with an emphasis on so‐called effector molecules that are secreted by the invading microbe to establish the symbiosis. However, the contribution of other types of molecules, such as glycans, is less well appreciated. Here, we present a random genetic screen that enabled us to identify 58 novel candidate genes that are involved in the pathogenic potential of the fungal pathogen Verticillium dahliae, which causes vascular wilt diseases in over 200 dicotyledonous plant species, including economically important crops. One of the candidate genes that was identified concerns a putative biosynthetic gene involved in nucleotide sugar precursor formation, as it encodes a putative nucleotide‐rhamnose synthase/epimerase‐reductase (NRS/ER). This enzyme has homology to bacterial enzymes involved in the biosynthesis of the nucleotide sugar deoxy‐thymidine diphosphate (dTDP)‐rhamnose, a precursor of L‐rhamnose, which has been shown to be required for virulence in several human pathogenic bacteria. Rhamnose is known to be a minor cell wall glycan in fungi and has therefore not been suspected as a crucial molecule in fungal–host interactions. Nevertheless, our study shows that deletion of the VdNRS/ER gene from the V. dahliae genome results in complete loss of pathogenicity on tomato and Nicotiana benthamiana plants, whereas vegetative growth and sporulation are not affected. We demonstrate that VdNRS/ER is a functional enzyme in the biosynthesis of uridine diphosphate (UDP)‐rhamnose, and further analysis has revealed that VdNRS/ER deletion strains are impaired in the colonization of tomato roots. Collectively, our results demonstrate that rhamnose, although only a minor cell wall component, is essential for the pathogenicity of V. dahliae. 相似文献
854.
Syeda Shahnaz Parvez Mohammad Masud Parvez Yoshiharu Fujii Hiroshi Gemma 《Plant Growth Regulation》2004,42(3):245-252
Allelopathic performance of the bark and seed of Tamarindus indica L. tree was evaluated through bioassay-guided studies using seven common agronomic crops (asparagus, cucumber, lettuce, radish,
sesame, tomato and welsh onion) and seven weed species (barnyard grass, Chinese milk vetch, perennial ryegrass, phacelia,
timothy grass, white clover and wild ginger) under laboratory conditions. As demonstrated by a sandwich method, the bark of
the tamarind tree caused strong growth inhibition (compared to the corresponding controls) in both radicles and hypocotyls
of the species tested, and the inhibitory effect was highest in barnyard grass (52–65%) and lowest in welsh onion (19–13%).
The crude-water soluble extracts of bark at different concentrations (1, 5 and 10%) (w/v) exhibited a strong growth inhibition
in all the plant species tested, and a proportional increase in the percentage of growth inhibition was observed with an increase
in the concentrations of the extracts. The magnitude of inhibition in weed species was higher (5–60%) than those of agronomic
crop species (3–40%). The growth of all the weed species tested was strongly inhibited (17–56%), while the agronomic crop
species showed both inhibited (5–21%) and stimulated (5–27%) growth due to the effect of crude-water soluble exudates of tamarind
seed. Among the agronomic crop species tested, lettuce (22–27%) followed by radish (20–25%) and sesame (5–8%) showed stimulatory
growth with the crude-water soluble exudates of seed. In the pot culture experiments using four agronomic crops (lettuce,
radish, tomato and cucumber) and two weed species (barnyard grass and white clover), spraying of crude-water soluble extracts
of tamarind seed-coat at three different concentrations (1, 5 and 10%) (w/v) showed that the growth of lettuce (35–62%) and
radish (32–56%) was stimulated, while all other species tested showed growth inhibition (29–61%). When the spraying of crude
extracts of seed-coat was turned off, the growth of both lettuce and radish continued to be stimulated (4–7%) and all other
previously inhibited species recovered well, the recovery percentage ranging between 78 and 82%. However, when spraying of
crude extracts of seed-coat was continued, growth increased (10–14%) in lettuce and radish, and reduced (37–76%) in four other
species tested. The inhibitory or stimulatory effects of the crude extracts on agronomic crop and weed species were higher
in the radicle than the hypocotyl and reached a peak with 10% (w/v) concentrations. These results clearly demonstrated the
differential allelopathic effects (inhibitory and excitatory) of bark and seed of tamarind tree in the species tested. Thus,
it is evident that these two organs contain certain biologically active true growth regulator(s) and are either additively
or synergistically involved in the plant-specific expression, particularly by the seed-coat. 相似文献
855.
Bumblebee, Bombus terrestris queens undergo winter diapause and show a great difference in diapause duration in natural conditions. Queens emerged from diapause initiate colonies by producing a batch of diploid (fertilised) eggs that develop into workers. In this study we investigated the effects of both the duration of queen diapause (2, 3, 4, or 5 months) and colony size (artificially limited to 50, 100, 150, and 200 workers) on the number of sexuals (males or new queens — gynes) produced, when gynes are produced and the longevity of both the foundress queen and the colony. Both worker population and diapause duration showed significant effect on sexual gyne production, foundress queen longevity and colony longevity but their interaction effect was insignificant. The worker number and diapause duration, respectively showed significant effect on sexual male production and gyne emergence period, but their interaction effects were insignificant. 相似文献
856.
研究了体外培养一种孟加拉传统香蕉(Musa spp.Cv. Kanthali)的茎尖组织。茎尖的原始细胞表面经无菌处理(0.1%HgCl2处理12min) ,接种6~15d后外植体地下茎部分仍有微生物污染(大部分是细菌) ,杀死了85%的外植体。为确定无污染培养基,将等量外植体分别浸泡在含400mg/L氨苄青霉素和200mg/L庆大霉素(两种光谱抗生素)的培养基中1h。结果表明,经抗生素处理的外植体完全没有污染,但培养3周后不能再生。进行二次继代培养后,其中一部分外植体吸收了培养基并胀大,颜色由苍白转变成浅绿或深绿。三次继代培养后数天,不再观察到外植体的生长,所有经抗生素处理过的外植体都开始死亡。在未经抗生素处理的活外植体中,单个茎发育的最佳培养基是:MS 4.0mg/L BA 0.5mg/L KT 15% CW,平均生长时间为18~21d,但再生率很低,只有30%。茎细胞增殖的最佳培养基是:MS 4.0mg/L BA 2.0mg/LIAA 15% CW,每个茎平均只萌发3~4个芽。最后,在添加0.5mg/LIBA的一半浓度的MS培养基中,体外培养茎最大生根率达到90%。 相似文献
857.
Md. Shahidul Islam 《Phytomedicine》2011,19(1):25-31
White tea (WT) is very similar to green tea (GT) but it is exceptionally prepared only from the buds and young tea leaves of Camelia sinensis plant while GT is prepared from the matured tea leaves. The present study was investigated to examine the effects of a 0.5% aqueous extract of WT in a streptozotocin-induced diabetes model of rats. Six-week-old male Sprague-Dawley rats were divided into 3 groups of 6 animals in each group namely: normal control (NC), diabetic control (DBC) and diabetic white tea (DWT). Diabetes was induced by an intraperitoneal injection of streptozotocin (65 mg/kg BW) in DBC and DWT groups except the NC group. After 4 weeks feeding of 0.5% aqueous extracts of WT, the drink intake was significantly (P < 0.05) increased in the DWT group compared to the DBC and NC groups. Blood glucose concentrations were significantly decreased and glucose tolerance ability was significantly improved in the DWT group compared to the DBC group. Liver weight and liver glycogen were significantly increased and serum total cholesterol and LDL-cholesterol were significantly decreased in the DWT group compared to the DBC group. The food intake, body weight gain, serum insulin and fructosamine concentrations were not influenced by the consumption of WT. Data of this study suggest that the 0.5% aqueous extract of WT is effective to reduce most of the diabetes associated abnormalities in a steptozotocin-induced diabetes model of rats. 相似文献
858.
859.
Aung Ko Ko Oo Anna Sanchez Calle Neha Nair Hafizah Mahmud Arun Vaidyanath Junya Yamauchi Aprilliana Cahya Khayrani Juan Du Md Jahangir Alam Akimasa Seno Akifumi Mizutani Hiroshi Murakami Yoshiaki Iwasaki Ling Chen Tomonari Kasai Masaharu Seno 《Translational oncology》2018,11(3):653-663
Previously, we have succeeded in converting induced pluripotent stem cells (iPSCs) into cancer stem cells (CSCs) by treating the iPSCs with conditioned medium of Lewis lung carcinoma (LLC) cells. The converted CSCs, named miPS-LLCcm cells, exhibited the self-renewal, differentiation potential, and potential to form malignant tumors with metastasis. In this study, we further characterized miPS-LLCcm cells both in vivo and in vitro. The tumors formed by subcutaneous injection showed the structures with pathophysiological features consisting of undifferentiated and malignant phenotypes generally found in adenocarcinoma. Metastasis in the lung was also observed as nodule structures. Excising from the tumors, primary cultured cells from the tumor and the nodule showed self-renewal, differentiation potential as well as tumor forming ability, which are the essential characters of CSCs. We then characterized the epigenetic regulation occurring in the CSCs. By comparing the DNA methylation level of CG rich regions, the differentially methylated regions (DMRs) were evaluated in all stages of CSCs when compared with the parental iPSCs. In DMRs, hypomethylation was found superior to hypermethylation in the miPS-LLCcm cells and its derivatives. The hypo- and hypermethylated genes were used to nominate KEGG pathways related with CSC. As a result, several categories were defined in the KEGG pathways from which most related with cancers, significant and high expression of components was PI3K-AKT signaling pathway. Simultaneously, the AKT activation was also confirmed in the CSCs. The PI3K-Akt signaling pathway should be an important pathway for the CSCs established by the treatment with conditioned medium of LLC cells. 相似文献
860.
Myong-Kyun Roh Md. Salim Uddin Byung-Soo Chun 《Biotechnology and Bioprocess Engineering》2008,13(6):724-729
Extraction of brown seaweed (Undaria pinnatifida) oil was carried out by using supercritical carbon dioxide (SCO2) and ethanol as co-solvent. The flow rate of ethanol was 3.0% (v/v) as compared to that of SCO2. Experiments were performed in a semi-batch flow apparatus on dried samples at temperatures from 303 to 333 K and pressures
from 80 to 300 bar. Fucoxanthin and polyphenol were quantitatively analyzed by using HPLC and UV-spectrometer. The highest
yields of fucoxanthin and polyphenol were shown at 200 bar, 323 K and 250 bar, 333 K, respectively. The solubility of fucoxanthin
in SCO2 agreed well with the Chrastil model. 相似文献