Brain development: neural signature predicts autism's emergence

A new study has found that neural sensitivity to eye gaze in early infancy is associated with subsequent development of autism. This discovery provides a much-needed biomarker for autism spectrum disorder prior to emergence of behavioral symptoms.     

Coevolution between cannabinoid receptors and endocannabinoid ligands

Genes for receptors and ligands must coevolve to maintain coordinated gene expression and binding affinities. Researchers have debated whether anandamide or 2-arachidonyl glycerol (2-AG) is a more "intrinsic" ligand of cannabinoid receptors. We addressed this debate with a coevolutionary analysis, by examining genes for CB1, CB2, and ten genes that encode ligand metabolic enzymes: abhydrolase domain containing 4 protein, cyclooxygenase 2, diacylglycerol lipase paralogs (DAGLalpha, DAGLbeta), fatty acid amide hydrolase paralogs (FAAH1, FAAH2), monoglyceride lipase, N-acylethanolamine acid amidase, NAPE-selective phospholipase D, and protein tyrosine phosphatase non-receptor type 22. Gene trees (cladograms) of CB1, CB2, and ligand enzymes were obtained by searching for orthologs (tBLASTn) in the genomes of nine phylogenetically diverse species, aligning ortholog sequences with ClustalX, and applying Bayesian analysis (MrBayes). Mirrored cladograms provided evidence of coevolution (i.e., parallel cladogenesis). Next we constructed phylograms of CB1, CB2, and the ten enzymes. Phylogram branch lengths were proportional to three sets of maximum likelihood metrics: all-nucleotide-substitutions and NS/SS ratios (using PAUP()), and Ka/Ks ratios (using FUGE). Spurious correlations in all-nucleotide-substitutions trees (due to phylogenetic bias) and in Ka/Ks ratio trees (due to simplistic modeling) were parsed. Branch lengths from equivalent branches in paired trees were correlated by linear regression. Regression analyses, mirrored cladograms, and phylogenetic profiles produced the same results: close associations between cannabinoid receptors and DAGL enzymes. Therefore we propose that cannabinoid receptors initially coevolved with a fatty acid ester ligand (akin to 2-AG) in ancestral metazoans, and affinity for fatty acid ethanolamide ligands (e.g., AEA) evolved thereafter.     

Value of a newly sequenced bacterial genome

Next-generation sequencing(NGS) technologies have made high-throughput sequencing available to medium- and small-size laboratories, culminating in a tidal wave of genomic information. The quantity of sequenced bacterial genomes has not only brought excitement to the field of genomics but also heightened expectations that NGS would boost antibacterial discovery and vaccine development. Although many possible drug and vaccine targets have been discovered, the success rate of genome-based analysis has remained below expectations. Furthermore, NGS has had consequences for genome quality, resulting in an exponential increase in draft(partial data) genome deposits in public databases. If no further interests are expressed for a particular bacterial genome, it is more likely that the sequencing of its genome will be limited to a draft stage, and the painstaking tasks of completing the sequencing of its genome and annotation will not be undertaken. It is important to know what is lost when we settle for a draft genome and to determine the "scientific value" of a newly sequenced genome. This review addresses the expected impact of newly sequenced genomes on antibacterial discovery and vaccinology. Also, it discusses the factors that could be leading to the increase in the number of draft deposits and the consequent loss of relevant biological information.     

Telemetric motor activity in children. A preliminary study

A lightweight telemetric mobility sensing system was used to study the relationship between high levels of motor activity during free-play and school performance. Among the 21 normal children, there was a significant correlation between high ankle motor activity during free-play, poor school achievement, the presence of neurological soft signs, and a poor self-image. Those normals whose free-play ankle activity was above the mean, also had significantly more errors and performed at a significantly lower level on the Bender Visual Motor Gestalt Test than children whose activity was below the mean. This preliminary study suggests that the telemetric mobility sensing system can be easily applied to children to assess clinically relevant components of psychomotor activity.     

Cannabis in Asia: its center of origin and early cultivation,based on a synthesis of subfossil pollen and archaeobotanical studies

Vegetation History and Archaeobotany - Biogeographers assign the Cannabis centre of origin to “Central Asia”, mostly based on wild-type plant distribution data. We sought greater...     

Evidence for a factor in liver cytosol which controls the competition between progesterone and triamcinolone acetonide for the glucocorticoid receptor

There is an inhibitory factor in rat liver which prevents progesterone and certain of its analogues from competing with triamcinolone acetonide for binding to the blucocorticoid receptor. The effects of this inhibitor can be eliminated by dilution of the cytosol or by gel filtration. Neither rat thymus nor serum contain this factor.     

A Functional Model for Studying Common Trends Across Trial Time in Eye Tracking Experiments

Eye tracking (ET) experiments commonly record the continuous trajectory of a subject’s gaze on a two-dimensional screen throughout repeated presentations of stimuli (referred to as trials). Even though the continuous path of gaze is recorded during each trial, commonly derived outcomes for analysis collapse the data into simple summaries, such as looking times in regions of interest, latency to looking at stimuli, number of stimuli viewed, number of fixations, or fixation length. In order to retain information in trial time, we utilize functional data analysis (FDA) for the first time in literature in the analysis of ET data. More specifically, novel functional outcomes for ET data, referred to as viewing profiles, are introduced that capture the common gazing trends across trial time which are lost in traditional data summaries. Mean and variation of the proposed functional outcomes across subjects are then modeled using functional principal component analysis. Applications to data from a visual exploration paradigm conducted by the Autism Biomarkers Consortium for Clinical Trials showcase the novel insights gained from the proposed FDA approach, including significant group differences between children diagnosed with autism and their typically developing peers in their consistency of looking at faces early on in trial time.