全文获取类型
收费全文 | 416篇 |
免费 | 75篇 |
专业分类
491篇 |
出版年
2023年 | 3篇 |
2021年 | 8篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 20篇 |
2014年 | 17篇 |
2013年 | 24篇 |
2012年 | 28篇 |
2011年 | 26篇 |
2010年 | 19篇 |
2009年 | 21篇 |
2008年 | 21篇 |
2007年 | 17篇 |
2006年 | 22篇 |
2005年 | 13篇 |
2004年 | 22篇 |
2003年 | 20篇 |
2002年 | 20篇 |
2001年 | 12篇 |
2000年 | 16篇 |
1999年 | 15篇 |
1998年 | 11篇 |
1997年 | 7篇 |
1996年 | 8篇 |
1994年 | 5篇 |
1993年 | 6篇 |
1992年 | 10篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 8篇 |
1988年 | 7篇 |
1987年 | 8篇 |
1986年 | 2篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 5篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1965年 | 1篇 |
1960年 | 1篇 |
1951年 | 1篇 |
1936年 | 1篇 |
1929年 | 1篇 |
1927年 | 1篇 |
1926年 | 1篇 |
1917年 | 1篇 |
1915年 | 2篇 |
1913年 | 1篇 |
排序方式: 共有491条查询结果,搜索用时 15 毫秒
21.
Molecular architecture of the ribosome‐bound Hepatitis C Virus internal ribosomal entry site RNA 下载免费PDF全文
Justus Loerke Jochen Ismer Andrea Schmidt Tarek Hilal Thiemo Sprink Kaori Yamamoto Thorsten Mielke Jörg Bürger Tanvir R Shaikh Marylena Dabrowski Peter W Hildebrand Patrick Scheerer Christian MT Spahn 《The EMBO journal》2015,34(24):3042-3058
Internal ribosomal entry sites (IRESs) are structured cis‐acting RNAs that drive an alternative, cap‐independent translation initiation pathway. They are used by many viruses to hijack the translational machinery of the host cell. IRESs facilitate translation initiation by recruiting and actively manipulating the eukaryotic ribosome using only a subset of canonical initiation factor and IRES transacting factors. Here we present cryo‐EM reconstructions of the ribosome 80S‐ and 40S‐bound Hepatitis C Virus (HCV) IRES. The presence of four subpopulations for the 80S•HCV IRES complex reveals dynamic conformational modes of the complex. At a global resolution of 3.9 Å for the most stable complex, a derived atomic model reveals a complex fold of the IRES RNA and molecular details of its interaction with the ribosome. The comparison of obtained structures explains how a modular architecture facilitates mRNA loading and tRNA binding to the P‐site. This information provides the structural foundation for understanding the mechanism of HCV IRES RNA‐driven translation initiation. 相似文献
22.
23.
McNally FJ 《Current biology : CB》1999,9(8):R274-R276
The rapid switching between growth and shrinkage at microtubule ends is important for many cellular processes. Recent studies on the structure of the microtubule and on the mechanism of action of the microtubule regulators XKCM1 and OP18 have revealed how these switching events are regulated. 相似文献
24.
Three-dimensional imaging by deconvolution microscopy 总被引:26,自引:0,他引:26
Deconvolution is a computational method used to reduce out-of-focus fluorescence in three-dimensional (3D) microscope images. It can be applied in principle to any type of microscope image but has most often been used to improve images from conventional fluorescence microscopes. Compared to other forms of 3D light microscopy, like confocal microscopy, the advantage of deconvolution microscopy is that it can be accomplished at very low light levels, thus enabling multiple focal-plane imaging of light-sensitive living specimens over long time periods. Here we discuss the principles of deconvolution microscopy, describe different computational approaches for deconvolution, and discuss interpretation of deconvolved images with a particular emphasis on what artifacts may arise. 相似文献
25.
To investigate myosin II function in cell movement within a cell mass, we imaged green fluorescent protein-myosin heavy chain (GFP-MHC) cells moving within the tight mound of Dictyostelium discoideum. In the posterior cortex of cells undergoing rotational motion around the center of the mound, GFP-MHC cyclically formed a "C," which converted to a spot as the cell retracted its rear. Consistent with an important role for myosin in rotation, cells failed to rotate when they lacked the myosin II heavy chain (MHC-) or when they contained predominantly monomeric myosin II (3xAsp). In cells lacking the myosin II regulatory light chain (RLC-), rotation was impaired and eventually ceased. These rotational defects reflect a mechanical problem in the 3xAsp and RLC- cells, because these mutants exhibited proper rotational guidance cues. MHC- cells exhibited disorganized and erratic rotational guidance cues, suggesting a requirement for the MHC in organizing these signals. However, the MHC- cells also exhibited mechanical defects in rotation, because they still moved aberrantly when seeded into wild-type mounds with proper rotational guidance cues. The mechanical defects in rotation may be mediated by the C-to-spot, because RLC- cells exhibited a defective C-to-spot, including a slower C-to-spot transition, consistent with this mutant's slower rotational velocity. 相似文献
26.
27.
28.
29.
McNally F 《Current biology : CB》2003,13(15):R597-R599
Dis1/XMAP215 family microtubule-binding proteins are essential for cell division in animals, plants and fungi, suggesting a conserved cell-division mechanism used by all eukaryotes. Two new studies, however, reveal that different family members can have very different effects on microtubule dynamics. 相似文献
30.
Mislow JM Holaska JM Kim MS Lee KK Segura-Totten M Wilson KL McNally EM 《FEBS letters》2002,519(1-3):135-140
The mitogen-stimulated protein kinase p70(s6k)/p85(s6k) (S6K) plays an essential role in cell proliferation and growth, with inhibitors of the S6K signalling pathway showing promise as anti-tumour therapeutics. Here, we report that the bisindolylmaleimide derivative Ro 31-6045, previously reported to be inactive as a kinase inhibitor, inhibited S6K activity in vivo with an IC50=8 microM. Structure/function analysis using mutant forms of S6K indicates that Ro 31-6045 inhibition is independent of the upstream activator mTOR. Ro 31-6045 will prove useful in elucidating the complex activation mechanism of S6K and its independence from mTOR will allow confirmation of functional data obtained using the mTOR inhibitor rapamycin. 相似文献