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611.
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Karen Overend Helen Lewis Della Bailey Kate Bosanquet Carolyn Chew-Graham David Ekers Samantha Gascoyne Deborah Hems John Holmes Ada Keding Dean McMillan Shaista Meer Natasha Mitchell Sarah Nutbrown Steve Parrott David Richards Gemma Traviss Dominic Trépel Rebecca Woodhouse Simon Gilbody 《Trials》2014,15(1)
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Sara K. McMillan Henry F. Wilson Christina L. Tague Daniel M. Hanes Shreeram Inamdar Diana L. Karwan Terry Loecke Jonathan Morrison Sheila F. Murphy Philippe Vidon 《Biogeochemistry》2018,141(3):487-501
While the influence of antecedent conditions on watershed function is widely recognized under typical hydrologic regimes, gaps remain in the context of extreme climate events (ECEs). ECEs are those events that far exceed seasonal norms of intensity, duration, or impact upon the physical environment or ecosystem. In this synthesis, we discuss the role of source availability and hydrologic connectivity on antecedent conditions and propose a conceptual framework to characterize system response to ECEs at the watershed scale. We present four case studies in detail that span a range of types of antecedent conditions and type of ECE to highlight important controls and feedbacks. Because ECEs have the potential to export large amounts of water and materials, their occurrence in sequence can disproportionately amplify the response. In fact, multiple events may not be considered extreme in isolation, but when they occur in close sequence they may lead to extreme responses in terms of both supply and transport capacity. Therefore, to advance our understanding of these complexities, we need continued development of a mechanistic understanding of how antecedent conditions set the stage for ECE response across multiple regions and climates, particularly since monitoring of these rare events is costly and difficult to obtain. Through focused monitoring of critical ecosystems during rare events we will also be able to extend and validate modeling studies. Cross-regional comparisons are also needed to define characteristics of resilient systems. These monitoring, modeling, and synthesis efforts are more critical than ever in light of changing climate regimes, intensification of human modifications of the landscape, and the disproportionate impact of ECEs in highly populated regions. 相似文献
615.
The food and feeding habits of black oreo and smooth oreo sampled at depths of 600–1200 m from the south-west Chatham Rise in 1983 are described, with notes on prey of eight other fish species. All these fish species fed benthopelagically. Black oreo preyed on hyperiid amphipods, salps and natant decapod crustaceans; smooth oreo on salps and amphipods. Dietary composition of both species changed with size of fish. Aspects of feeding relationships between the fish species were examined including dietary similarity and prey-size selection. Findings are compared with results of research on other New Zealand deepwater fish species. 相似文献
616.
The establishment of an ELISA for the detection of pregnancy-associated glycoproteins (PAGs) in the serum of pregnant cows and heifers 总被引:1,自引:0,他引:1
Green JA Parks TE Avalle MP Telugu BP McLain AL Peterson AJ McMillan W Mathialagan N Hook RR Xie S Roberts RM 《Theriogenology》2005,63(5):1481-1503
The pregnancy-associated glycoproteins (PAGs) are a large gene family expressed in trophoblast cells of ruminant ungulates. The detection of PAGs (more specifically, PAG-1) in maternal serum has served as the basis for pregnancy detection in cattle. Unfortunately, PAG-1 and/or antigenically-related PAGs exhibit a long half-life in maternal serum (>8 d) and can be detected 80-100 d post-partum, thereby producing false positives in animals bred within 60-d of calving. The goal of the present studies was to develop a monoclonal-based assay that targeted early-pregnancy PAGs whose persistence in maternal serum post-partum might be relatively short-lived. Three anti-PAG monoclonal antibodies that recognized distinct subsets of PAGs were selected and used as trapping reagents in a 'sandwich' type of enzyme-linked immunosorbant assay (ELISA). A polyclonal antiserum with broad specificity was used for detecting bound PAGs. A total of 42 cows and heifers were bled daily on day 15, days 22 to 28, and then weekly throughout pregnancy and for 10 weeks (approximately 70 d) into the post-partum period. The ELISA was able to detect PAG in maternal serum of all animals unambiguously by day 28 post-insemination (PAG concentration: 8.75 +/- 3.04 ng/mL). In maternal serum, PAG concentrations peaked during the week of parturition at 588.9 +/- 249.9 ng/mL, and after calving, PAG was completely cleared (half-life: 4.3 d) by eight-week post-partum in 38 of 40 of the animals tested and was at very low concentrations in the remaining two (1.4 and 4.9 ng/mL, respectively). In summary, a monoclonal-based assay has been established that is sensitive enough to detect PAG in maternal serum by the forth week of pregnancy, but does not suffer from carry-over of antigen from a previous pregnancy. 相似文献
617.
Heliconius melpomene is a mimetic butterfly that exhibits great geographic variation in color pattern. We present here a genetic linkage map based on analysis of genetic markers in 73 individuals from a single F(2) family, offspring of a cross between H. m. cythera from western Ecuador and H. m. melpomene from French Guiana. A novel "three-step method" is described for the analysis of dominant markers in an F(2) cross, using outbred parental strains and taking advantage of the lack of crossing over in female Lepidoptera. This method is likely to prove useful for future mapping studies in outbred species with crossing over restricted to one sex, such as the Lepidoptera and Drosophila. The resulting linkage map has 21 linkage groups corresponding to the 21 chromosomes of H. melpomene and includes 219 AFLP markers, 23 microsatellites, 19 single-copy nuclear genes, and the color pattern switch genes Yb and Sb. The marker density is high, averaging >1/7 cM. The total map length is 1616 cM and the average chromosome length is 77 cM. The genome size of H. melpomene was estimated to be 292 Mb, giving a relationship of physical-to-map distance of 180 kb/cM. This map forms the basis for future comparative linkage analysis of color pattern evolution in Heliconius. 相似文献
618.
Danyelle A. Ferreira Yaman Tayyar Adi Idris Nigel A.J. McMillan 《生物化学与生物物理学报:癌评论》2021,1875(1):188476
BackgroundIt is well-known that certain cancers are caused by viruses. However, viral oncogenesis is complex and only a small fraction of the infected people develop cancer. Indeed, a number of environmental factors can contribute to virally infected cells developing cancer hallmarks, promoting tumorigenesis.Scope of reviewThe hit-and-run theory proposes that viruses facilitate the accumulation of mutations and promote genomic instability until the virus becomes dispensable for tumour maintenance. Indeed, several studies have reported viral genome, episome and/or oncogene loss in tumour cells without losing malignant phenotype.Major conclusionsThe current evidence supports the clear contribution of certain viruses to develop cancers. Importantly, the evidence supporting the sustained maintenance of malignancy after the loss of viral “presence” is sufficient to support the hit-and-run hypothesis of viral cancer development. Long-term tracking of vaccination outcome over the decades will test this theory.General significanceIf the hit-and-run theory is true, viruses might cause more cancers than previously thought and will have implications in the prevention of many cancers through implementing vaccination programs. 相似文献
619.
Studies using molecular markers have shown that some grey seal males may be gaining success through exhibiting alternative mating tactics. We estimated the probability of fertilization success of grey seal males exhibiting the primary tactic of female defence and one alternative tactic of mating with departing females on Sable Island, Nova Scotia, Canada, during the breeding seasons of 1997-2002. Although the fertilization rate of the primary tactic (27-43%) was greater than that of the alternative tactic (10-12%), these low rates indicate the potential fitness value of alternative mating tactics in this size-dimorphic pinniped species. 相似文献
620.
Laboratory-germinated seedlings from Connecticut, New Jersey, Illinois, Tennessee, Texas, and Florida were compared under four photoperiod temperature programs. Under each controlled condition, seedlings of northernmost provenances demonstrated least stem elongation and earliest dormancy. Greatest sensitivity to cool temperatures and short photoperiods was demonstrated by northernmost provenances. At warm temperatures, dormant bud formation was controlled by photoperiod in all populations tested except those from Texas and Florida. The reported differences among populations reflect the effects of natural selection upon different Liquidambar populations, insuring survival of the species in various habitats. 相似文献