全文获取类型
收费全文 | 13776篇 |
免费 | 1184篇 |
国内免费 | 7篇 |
专业分类
14967篇 |
出版年
2022年 | 88篇 |
2021年 | 178篇 |
2020年 | 93篇 |
2019年 | 150篇 |
2018年 | 137篇 |
2017年 | 121篇 |
2016年 | 273篇 |
2015年 | 475篇 |
2014年 | 528篇 |
2013年 | 725篇 |
2012年 | 864篇 |
2011年 | 875篇 |
2010年 | 555篇 |
2009年 | 528篇 |
2008年 | 755篇 |
2007年 | 760篇 |
2006年 | 666篇 |
2005年 | 734篇 |
2004年 | 700篇 |
2003年 | 635篇 |
2002年 | 637篇 |
2001年 | 141篇 |
2000年 | 126篇 |
1999年 | 151篇 |
1998年 | 182篇 |
1997年 | 119篇 |
1996年 | 110篇 |
1995年 | 98篇 |
1994年 | 100篇 |
1993年 | 92篇 |
1992年 | 105篇 |
1991年 | 95篇 |
1990年 | 86篇 |
1989年 | 82篇 |
1988年 | 84篇 |
1987年 | 94篇 |
1986年 | 92篇 |
1985年 | 87篇 |
1984年 | 138篇 |
1983年 | 102篇 |
1982年 | 126篇 |
1981年 | 133篇 |
1980年 | 147篇 |
1979年 | 118篇 |
1978年 | 106篇 |
1977年 | 111篇 |
1976年 | 88篇 |
1975年 | 92篇 |
1974年 | 105篇 |
1973年 | 76篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
Diwen Liang George B. McManus Qing Wang Xian Sun Zhiwei Liu Senjie Lin Yufeng Yang 《Ecology and evolution》2022,12(5)
Genetic differentiations and phylogeographical patterns of small organisms may be shaped by spatial isolation, environmental gradients, and gene flow. However, knowledge about genetic differentiation of rotifers at the intercontinental scale is still limited. Polyarthra dolichoptera and P. vulgaris are cosmopolitan rotifers that are tolerant to environmental changes, offering an excellent model to address the research gap. Here, we investigated the populations in Southeastern China and eastern North America and evaluated the phylogeographical patterns from their geographical range sizes, geographic–genetic distance relationships and their responses to spatial‐environmental factors. Using the mitochondrial cytochrome c oxidase subunit I gene as the DNA marker, we analyzed a total of 170 individuals. Our results showed that some putative cryptic species, also known as entities were widely distributed, but most of them were limited to single areas. The divergence of P. dolichoptera and P. vulgaris indicated that gene flow between continents was limited while that within each continent was stronger. Oceanographic barriers do affect the phylogeographic pattern of rotifers in continental waters and serve to maintain genetic diversity in nature. The genetic distance of P. dolichoptera and P. vulgaris populations showed significant positive correlation with geographic distance. This might be due to the combined effects of habitat heterogeneity, long‐distance colonization, and oceanographic barriers. Furthermore, at the intercontinental scale, spatial distance had a stronger influence than environmental variables on the genetic differentiations of both populations. Wind‐ and animal‐mediated transport and even historical events of continental plate tectonics are potential factors for phylogeography of cosmopolitan rotifers. 相似文献
92.
93.
The energetic incubation constraint hypothesis (EICH) for clutch size states that birds breeding in poor habitat may free
up resources for future reproduction by laying a smaller clutch. The eider (Somateria mollissima) is considered a candidate for supporting this hypothesis. Clutch size is smaller in exposed nests, presumably because of
faster heat loss and higher incubation cost, and, hence, smaller optimal clutch size. However, an alternative explanation
is partial predation: the first egg(s) are left unattended and vulnerable to predation, which may disproportionately affect
exposed nests, so clutch size may be underestimated. We experimentally investigated whether predation on first-laid eggs in
eiders depends on nest cover. We then re-evaluated how nesting habitat affects clutch size and incubation costs based on long-term
data, accounting for confounding effects between habitat and individual quality. We also experimentally assessed adult survival
costs of nesting in sheltered nests. The risk of egg predation in experimental nests decreased with cover. Confounding between
individual and habitat quality is unlikely, as clutch size was also smaller in open nests within individuals, and early and
late breeders had similar nest cover characteristics. A trade-off between clutch and female safety may explain nest cover
variation, as the risk of female capture by us, mimicking predation on adults, increased with nest cover. Nest habitat had
no effect on female hatching weight or weight loss, while lower temperature during incubation had an unanticipated positive
relationship with hatching weight. There were no indications of elevated costs of incubating larger clutches, while clutch
size and colony size were positively correlated, a pattern not predicted by the ‘energetic incubation constraint’ hypothesis.
Differential partial clutch predation thus offers the more parsimonious explanation for clutch size variation among habitats
in eiders, highlighting the need for caution when analysing fecundity and associated life-history parameters when habitat-specific
rates of clutch predation occur. 相似文献
94.
95.
96.
Edward J. Noga Anirudh J. Ullal Jone Corrales Jorge M.O. Fernandes 《Comparative biochemistry and physiology. Part D, Genomics & proteomics》2011,6(1):44-54
Antimicrobial polypeptides (AMPPs), consisting of peptides and small proteins with antimicrobial activity, are an integral component of innate immunity. Their often potent properties and widespread prevalence in fish suggests that designing means of manipulating their levels has considerable potential for maintaining or improving fish health. There is evidence that a number of chronic stresses lead to significant downregulation of AMPPs and thus their monitoring could be a highly sensitive measure of health status and risk of an infectious disease outbreak. Conversely, upregulation of AMPP expression could be used to enhance disease resistance in stressful environments, as well as improve the efficacy of traditional antimicrobial drugs. However, further work is required in linking levels of a number of AMPPs to physiological function since, while a number of studies have documented the down- or upregulation of AMPPs via gene expression, relatively few studies have quantitatively examined changes in protein expression. In addition, not all AMPPs appear to be expressed at microbicidal levels in vivo, suggesting that at least some may have functions other than being directly protective. Nonetheless, in fish, there is evidence that some constitutively expressed AMPPs, such as piscidins and histone-like proteins, are expressed at microbicidal levels and that they decline with stress. Furthermore, certain AMPPs derived from hemoglobin-β are upregulated to microbicidal levels after experimental challenge. The likely widespread distribution of these three AMPP groups in fish provides the opportunity to design strategies to greatly improve the health of cultured fish populations. 相似文献
97.
Tsunenori Nozawa Jeffrey T. Trost Taisei Fukada Masahiro Hatano James D. McManus Robert E. Blankenship 《BBA》1987,894(3):468-476
Reaction centers were purified from the thermophilic purple sulfur photosynthetic bacterium Chromatium tepidum. The reaction center consists of four polypeptides L, M, H and C, whose apparent molecular masses were determined to be 25, 30, 34 and 44 kDa, respectively, by polyacrylamide gel electrophoresis. The heaviest peptide corresponds to tightly bound cytochrome. The tightly bound cytochrome c contains two types of heme, high-potential c-556 and low-potential c-553. The low-potential heme is able to be photooxidized at 77 K. The reaction center exhibits laser-flash-induced absorption changes and circular dichroism spectra similar to those observed in other purple photosynthetic bacteria. Whole cells contain both ubiquinone and menaquinone. Reaction centers contain only a single active quinone; chemical analysis showed this to be menaquinone. Reaction center complexes without the tightly bound cytochrome were also prepared. The near-infrared pigment absorption bands are red-shifted in reaction centers with cytochrome compared to those without cytochrome. 相似文献
98.
Xie LX Hsieh EJ Watanabe S Allan CM Chen JY Tran UC Clarke CF 《Biochimica et biophysica acta》2011,1811(5):348-360
Coenzyme Q (ubiquinone or Q) is a lipid electron and proton carrier in the electron transport chain. In yeast Saccharomyces cerevisiae eleven genes, designated COQ1 through COQ9, YAH1 and ARH1, have been identified as being required for Q biosynthesis. One of these genes, COQ8 (ABC1), encodes an atypical protein kinase, containing six (I, II, III, VIB, VII, and VIII) of the twelve motifs characteristically present in canonical protein kinases. Here we characterize seven distinct Q-less coq8 yeast mutants and show that unlike the coq8 null mutant, each maintained normal steady-state levels of the Coq8 polypeptide. The phosphorylation states of Coq polypeptides were determined with two-dimensional gel analyses. Coq3p, Coq5p, and Coq7p were phosphorylated in a Coq8p-dependent manner. Expression of a human homolog of Coq8p, ADCK3(CABC1) bearing an amino-terminal yeast mitochondrial leader sequence, rescued growth of yeast coq8 mutants on medium containing a nonfermentable carbon source and partially restored biosynthesis of Q(6). The phosphorylation state of several of the yeast Coq polypeptides was also rescued, indicating a profound conservation of yeast Coq8p and human ADCK3 protein kinase function in Q biosynthesis. 相似文献
99.
Maria C Romay Mark J Millard Jeffrey C Glaubitz Jason A Peiffer Kelly L Swarts Terry M Casstevens Robert J Elshire Charlotte B Acharya Sharon E Mitchell Sherry A Flint-Garcia Michael D McMullen James B Holland Edward S Buckler Candice A Gardner 《Genome biology》2013,14(6):R55
Background
Genotyping by sequencing, a new low-cost, high-throughput sequencing technology was used to genotype 2,815 maize inbred accessions, preserved mostly at the National Plant Germplasm System in the USA. The collection includes inbred lines from breeding programs all over the world.Results
The method produced 681,257 single-nucleotide polymorphism (SNP) markers distributed across the entire genome, with the ability to detect rare alleles at high confidence levels. More than half of the SNPs in the collection are rare. Although most rare alleles have been incorporated into public temperate breeding programs, only a modest amount of the available diversity is present in the commercial germplasm. Analysis of genetic distances shows population stratification, including a small number of large clusters centered on key lines. Nevertheless, an average fixation index of 0.06 indicates moderate differentiation between the three major maize subpopulations. Linkage disequilibrium (LD) decays very rapidly, but the extent of LD is highly dependent on the particular group of germplasm and region of the genome. The utility of these data for performing genome-wide association studies was tested with two simply inherited traits and one complex trait. We identified trait associations at SNPs very close to known candidate genes for kernel color, sweet corn, and flowering time; however, results suggest that more SNPs are needed to better explore the genetic architecture of complex traits.Conclusions
The genotypic information described here allows this publicly available panel to be exploited by researchers facing the challenges of sustainable agriculture through better knowledge of the nature of genetic diversity. 相似文献100.
Elik Aharonovsky Edward N. Trifonov 《Journal of biomolecular structure & dynamics》2013,31(3):237-242
Abstract Conserved protein sequence segments are commonly believed to correspond to functional sites in the protein sequence. A novel approach is proposed to profile the changing degree of conservation along the protein sequence, by evaluating the occurrence frequencies of all short oligopeptides of the given sequence in a large proteome database. Thus, a protein sequence conservation profile can be plotted for every protein. The profile indicates where along the sequences the potential functional (conserved) sites are located. The corresponding oligopeptides belonging to the sites are very frequent across many prokaryotic species. Analysis of a representative set of such profiles reveals a common feature of all examined proteins: they consist of sequence modules represented by the peaks of conservation. Typical size of the modules (peak-to-peak distance) is 25–30 amino acid residues. 相似文献