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991.
992.
Lake Hoare (77° 38 S, 162° 53 E) is a perennially ice-covered lake at the eastern end of Taylor Valley in southern Victoria Land, Antarctica. The environment of this lake is controlled by the relatively thick ice cover (3–5 m) which eliminates wind generated currents, restricts gas exchange and sediment deposition, and reduces light penetration. The ice cover is in turn largely controlled by the extreme seasonality of Antarctica and local climate. Lake Hoare and other dry valley lakes may be sensitive indicators of short term (< 100 yr) climatic and/or anthropogenic changes in the dry valleys since the onset of intensive exploration over 30 years ago. The time constants for turnover of the water column and lake ice are 50 and 10 years, respectively. The turnover time for atmospheric gases in the lake is 30–60 years. Therefore, the lake environment responds to changes on a 10–100 year timescale. Because the ice cover has a controlling influence on the lake (e.g. light penetration, gas content of water, and sediment deposition), it is probable that small changes in ice ablation, sediment loading on the ice cover, or glacial meltwater (or groundwater) inflow will affect ice cover dynamics and will have a major impact on the lake environment and biota. 相似文献
993.
The thylakoid lamellae which traverse the pyrenoid of the unicellular red alga Porphyridium cruentum (Agardh) Nägeli appear to lack phycobilisomes. We have confirmed by immuno-electron microscopy that phycoerythrin (PE), an important structural component of the phycobilisomes of red algae, is absent from the pyrenoid. To characterize pyrenoid thylakoids further, electron-microscopic cytochemical methods were employed to detect photosystem activity. Photosystem (PS) I activity was demonstrated in both stromal and pyrenoid thylakoids by the photooxidation of 3,3-diaminobenzidine. In contrast, the localization of photoreduced distyryl nitroblue tetrazolium demonstrated that PSII activity was restricted to stromal thylakoids. The observed partitioning of PE and PSII activity within the plastid may be related to another observation, that being the localization of nearly all ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) within the pyrenoid of this alga. It is possible that the pyrenoid of P. cruentum functions as a specific metabolic compartment where CO2 fixation is enhanced by the absence of photosynthetic O2 evolution.Abbreviations DAB
3,3-diaminobenzidine-4HCl
- DS-NBT
distyryl nitroblue tetrazolium chloride
- EF
exoplasmic face
- LSU
large subunit of RuBisCO
- PE
phycoerythrin
- PS
photosystem
- RuBisCO
ribulose-1,5-bisphosphate carboxylase/oxygenase
We thank Drs. Jacqueline Fleck (CNRS, Strasbourg) and Robert MacColl (New York State Department of Health, Albany) for providing us with the antibodies used in this study. We also thank Dr. C.E. Smith for use of the Zeiss MOP-3 digital analyser and Dr. Geneviève Bricheux for kindly providing Lowicryl-embedded samples of P. cruentum. Aatrex® was kindly donated by Ciba-Geigy. This research was supported by the Natural Sciences and Engineering Research Council of Canada (grant No. A-2921). 相似文献
994.
M. C. McClure N. S. Morsci R. D. Schnabel J. W. Kim P. Yao M. M. Rolf S. D. McKay S. J. Gregg R. H. Chapple S. L. Northcutt J. F. Taylor 《Animal genetics》2010,41(6):597-607
To gain insight into the number of loci of large effect that underlie variation in cattle, a quantitative trait locus (QTL) scan for 14 economically important traits was performed in two commercial Angus populations using 390 microsatellites, 11 single nucleotide polymorphisms (SNPs) and one duplication loci. The first population comprised 1769 registered Angus bulls born between 1955 and 2003, with Expected Progeny Differences computed by the American Angus Association. The second comprised 38 half‐sib families containing 1622 steers with six post‐natal growth and carcass phenotypes. Linkage analysis was performed by half‐sib least squares regression with gridqtl or Bayesian Markov chain Monte Carlo analysis of complex pedigrees with loki . Of the 673 detected QTL, only 118 have previously been reported, reflecting both the conservative approach to QTL reporting in the literature, and the more liberal approach taken in this study. From 33 to 71% of the genetic variance and 35 to 56% of the phenotypic variance in each trait was explained by the detected QTL. To analyse the effects of 11 SNPs and one duplication locus within candidate genes on each trait, a single marker analysis was performed by fitting an additive allele substitution model in both mapping populations. There were 53 associations detected between the SNP/duplication loci and traits with ?log10Pnominal≥ 4.0, where each association explained 0.92% to 4.4% of the genetic variance and 0.01% to 1.86% of the phenotypic variance. Of these associations, only six SNP/duplication loci were located within 8 cM of a QTL peak for the trait, with two being located at the QTL peak: SST_DG156121:c.362A>G for ribeye muscle area and TG_X05380:c.422C>T for calving ease. Strong associations between several SNP/duplication loci and trait variation were obtained in the absence of any detected linked QTL. However, we reject the causality of several commercialized DNA tests, including an association between TG_X05380:c.422C>T and marbling in Angus cattle. 相似文献
995.
Bailey D. McKay 《Journal of avian biology》2009,40(2):181-190
Molecular tools are reshaping many traditional paradigms concerning the timeframe of avian diversification in North America. Phylogeographic studies have become essential for guiding the emerging paradigms. However, the current pool of such studies tends not to be evenly dispersed across the landscape, which limits the generality of inferences. The southeastern United States is one region where the tempo and mode of recent avian diversification is poorly understood. One phylogeographic break in particular, the Tombigbee River discontinuity, divides eastern and western phylogroups in Alabama, and, though it is known to have influenced the phylogeographic patterns of some fish and reptiles, its role in promoting recent avian diversification is not well understood. Eastern and western morphological subspecies of the yellow-throated warbler Dendroica dominica divide along the phylogeographic break created by the Tombigbee River discontinuity suggesting that this bird was also affected by this vicariance. To determine whether the phylogeographic patterns of the yellow-throated warbler are consistent with this biogeographic barrier, I analyzed mitochondrial control region sequences of 109 yellow-throated warblers from across the species' range and, from a subset of these samples, sequences from a sex-linked nuclear gene. Considerable variation was uncovered, but most of this variation was found within rather than among populations or subspecies. A shallow phylogenetic tree, star-like haplotype network, and unimodal mismatch distribution all suggested a recent expansion. Coalescent modeling indicated that modern populations are derived from a single common ancestral population and that differences among subspecies in morphology, ecology, and migratory pathways are the result of recent and rapid evolution, possibly driven by selection. 相似文献
996.
Russell L Legg Jessica R Tolman Cameron T Lovinger Edwin D Lephart Kenneth DR Setchell Merrill J Christensen 《Reproductive biology and endocrinology : RB&E》2008,6(1):57
Background
High dietary intake of selenium or soybean isoflavones reduces prostate cancer risk. These components each affect androgen-regulated gene expression. The objective of this work was to determine the combined effects of selenium and isoflavones on androgen-regulated gene expression in rat prostate. 相似文献997.
998.
McKay JK Richards JH Nemali KS Sen S Mitchell-Olds T Boles S Stahl EA Wayne T Juenger TE 《Evolution; international journal of organic evolution》2008,62(12):3014-3026
Despite compelling evidence that adaptation to local climate is common in plant populations, little is known about the evolutionary genetics of traits that contribute to climatic adaptation. A screen of natural accessions of Arabidopsis thaliana revealed Tsu-1 and Kas-1 to be opposite extremes for water-use efficiency and climate at collection sites for these accessions differs greatly. To provide a tool to understand the genetic basis of this putative adaptation, Kas-1 and Tsu-1 were reciprocally crossed to create a new mapping population. Analysis of F(3) families showed segregating variation in both delta(13)C and transpiration rate, and as expected these traits had a negative genetic correlation (r(g)=- 0.3). 346 RILs, 148 with Kas-1 cytoplasm and 198 with Tsu-1 cytoplasm, were advanced to the F(9) and genotyped using 48 microsatellites and 55 SNPs for a total of 103 markers. This mapping population was used for QTL analysis of delta(13)C using F(9) RIL means. Analysis of this reciprocal cross showed a large effect of cytoplasmic background, as well as two QTL for delta(13)C. The Kas-1 x Tsu-1 mapping population provides a powerful new resource for mapping QTL underlying natural variation and for dissecting the genetic basis of water-use efficiency differences. 相似文献
999.
Wigglesworth MJ Lawless KJ Standing DJ Mackenzie EK Kitchen VR McKay F Ward E Brough SJ Stylianou M Jewitt FR McLaren-Douglas AM Jowet MI Tamayama N Finnigan D Ding J Wise A 《Journal of biomolecular screening》2008,13(5):354-362
Measurement of intracellular calcium release following agonist challenge within cells expressing the relevant membrane protein is a commonly used format to derive structure-activity relationship (SAR) data within a compound profiling assay. The Fluorometric Imaging Plate Reader (FLIPR) has become the gold standard for this purpose. FLIPR traditionally uses cells that are maintained in continuous culture for compound profiling of iterative chemistry campaigns. This supply dictates that assays can only be run on 4 of 5 weekdays, or alternative cell culture machinery is required such that plating can occur remotely at the weekend. The data reported here demonstrate that high-quality compound profiling data can be generated from the use of cryopreserved cells and that these cells can also be plated at various densities to generate equivalent data between 24 and 72 h post-plating. Hence, the authors report a method that allows data generation throughout the week and without the requirement of highly automated cell culture or continuous culture. 相似文献
1000.
Hasmik Keshishian E Robert McDonald III Filip Mundt Randy Melanson Karsten Krug Dale A Porter Luke Wallace Dominique Forestier Bokang Rabasha Sara E Marlow Judit JaneValbuena Ellen Todres Harrison Specht Margaret Lea Robinson Pierre M Jean Beltran Ozgun Babur Meagan E Olive Javad Golji Eric Kuhn Michael Burgess Melanie A MacMullan Tomas Rejtar Karen Wang DR Mani Shankha Satpathy Michael A Gillette William R Sellers Steven A Carr 《Molecular systems biology》2021,17(9)
Reliable methods to quantify dynamic signaling changes across diverse pathways are needed to better understand the effects of disease and drug treatment in cells and tissues but are presently lacking. Here, we present SigPath, a targeted mass spectrometry (MS) assay that measures 284 phosphosites in 200 phosphoproteins of biological interest. SigPath probes a broad swath of signaling biology with high throughput and quantitative precision. We applied the assay to investigate changes in phospho‐signaling in drug‐treated cancer cell lines, breast cancer preclinical models, and human medulloblastoma tumors. In addition to validating previous findings, SigPath detected and quantified a large number of differentially regulated phosphosites newly associated with disease models and human tumors at baseline or with drug perturbation. Our results highlight the potential of SigPath to monitor phosphoproteomic signaling events and to nominate mechanistic hypotheses regarding oncogenesis, response, and resistance to therapy. 相似文献