尿液转流在尿道下裂术后复杂性尿瘘术中的应用

目的:评价尿液转流在修复尿道下裂术后复杂性尿瘘术中的作用。方法:将40例尿道下裂术后复杂性尿瘘患者随机分为尿液转流组和非尿液转流组两组进行比较。结果:尿液转流组:25例患者术后尿道皮肤瘘复发两例,手术成功率99.2%。非尿液转流组:15例患者中有6例(40%)发生尿瘘复发。结论:尿道下裂术后复杂性尿瘘修复术中应用尿液转流有较好的效果。     

谈“教学型高校”加大科研力度的必要性

’教学型’高校教师的主要工作是教学,科研工作处于时断时续的状态。针对以上现实,本文指出了"教学型高校"加大科研力度的必要性,并就目前的科研现实提出切实可行的改进措施。①教学与科研不是独立的两个方面,而是相互促进的一个整体,加大’教学型’高校的科研力度可以达到教学、科研的互利双赢。科研应该逐渐成为’教学型高校’教师的重要工作内容。②重视科研也是重视教学,提高教学质量以及围绕教学进行的评估、教改等各项举措,也应该从加大科研力度的角度进行:教师有了科研工作,自然需要学生参与;而学生加入到教师的科研活动,不仅是对学生动手能力、创新意识的最好培养,更将明显提高学生学习理论知识的积极性,提高听课效率。③改善’教学型高校’科研工作的现状,可从设立研究型教师岗位和减少行政指令性活动等方面入手。     

原发性十二指肠恶性肿瘤的临床分析

目的:探讨原发性十二指肠恶性肿瘤的临床特点、诊断方法和预后影响因素。方法:回顾性分析随访资料完整的45例原发性十二指肠恶性肿瘤患者的临床病理资料。结果:腺癌33例(73.3%)为主要的病理类型。主要临床表现为腹痛、上腹部不适、黄疸、消化道出血等。胃十二指肠镜、内镜逆行胰胆管造影(Endoscopic Retrograde Cholangio-Pancreatography,ERCP)、十二指肠低张造影、超声内镜、CT及B超确诊率分别为91.1%(41/45),93.3%(42/45),82.2%(37/45),75.6%(34/45),68.9%(31/45)及26.7%(12/45)。本组45例均行开腹手术,包括根治性手术,胰十二指肠切除术36例;姑息性手术,胃肠吻合术2例、肿瘤局部切除术5例、短路手术2例。根治术和姑息术后5年生存率分别为46.7%和4.4%,两组生存率差异有统计学意义(P<0.05)。对全组45例患者的预后因素进行Cox回归分析的结果显示,手术方式、肿瘤浸润深度和淋巴节转移是影响预后的独立危险因素(均P<0.05)。结论:原发性十二指肠恶性肿瘤缺乏特异性临床表现;胃十二指肠镜、ERCP以及十二指肠低张造影等联合检查可提高诊断率;根治性手术远期疗效较好;淋巴结转移和局部侵犯是肿瘤预后不良的重要影响因素。     

甲钴胺联合高能激光治疗带状疱疹疗效观察及护理体会

目的:探讨甲钴胺联合高能激光治疗带状疱疹疗效及护理方法。方法:自2008年1月至2010年3月入院的81例带状疱疹患者分为两组,治疗组予甲钴胺、单磷酸阿糖腺苷和高能激光联合治疗并配合精心护理,对照组仅予单磷酸阿糖腺苷治疗,两组均在治疗第10天时观察症状及体征的变化。患者出院后随访半年。结果:治疗组与对照组有效率分别为90.2%和56.4%,差异有统计学意义(P<0.05)。治疗组与对照组的止疱时间(1.4±1.1天vs2.6±1.3天)、结痂时间(5.4±1.4天vs8.1±1.5天)和止痛时间(3.0±1.6天vs 5.8±3.3天),以及治疗后两组患者直观模拟尺评分结果(2.95±1.45 vs 3.97±1.85)差异有统计学意义(P<0.05)。随访半年结果治疗组出现后遗神经痛较对照组少(P<0.05)。结论:甲钴胺联合高能激光治疗带状疱疹并配合精心护理有利于取得良好的临床治疗效果。     

彩色多普勒超声对晚孕期羊水过少胎儿肾动脉血流的研究

目的:应用彩色多普勒血流显像(CDFI)观察晚孕期羊水过少胎儿肾动脉(RA)血流阻力参数的变化,为临床预测羊水过少时胎儿宫内缺氧及评价胎盘功能提供相关信息。方法:应用CDFI检测100例晚孕期孕妇(孕36～40周)RA的搏动指数(PI)、阻力指数(RI)及收缩期峰值流速与舒张末期流速比值(S/D)。其中羊水过少A组(5cm<羊水指数AFI≤8cm)40例,羊水过少B组(AFI≤5 cm)20例,正常对照C组(8cm相似文献   

ZmJAZ与ZmMYC2的BiFC互作研究

JAZ(jasmonate ZIM-domain)蛋白是茉莉酸(jasmonic acid,JA)信号途径中关键的负调控因子,明确JAZ蛋白和MYC2之间的结合关系对整个JA信号通路至关重要.通过qRT-PCR筛选了在籽粒中较特异表达的ZmJAZ4、生殖器官中高表达的ZmJAZ8以及组成型表达的ZmJAZ12,利用玉米...     

Atomic force microscopy of a hybrid high-molecular-weight glutenin subunit from a transgenic hexaploid wheat

The high-molecular-weight glutenin subunits (HMW-GS) of wheat gluten in their native form are incorporated into an intermolecularly disulfide-linked, polymeric system that gives rise to the elasticity of wheat flour doughs. These protein subunits range in molecular weight from about 70 K-90 K and are made up of small N-terminal and C-terminal domains and a large central domain that consists of repeating sequences rich in glutamine, proline, and glycine. The cysteines involved in forming intra- and intermolecular disulfide bonds are found in, or close to, the N- and C-terminal domains. A model has been proposed in which the repeating sequence domain of the HMW-GS forms a rod-like beta-spiral with length near 50 nm and diameter near 2 nm. We have sought to examine this model by using noncontact atomic force microscopy (NCAFM) to image a hybrid HMW-GS in which the N-terminal domain of subunit Dy10 has replaced the N-terminal domain of subunit Dx5. This hybrid subunit, coded by a transgene overexpressed in transgenic wheat, has the unusual characteristic of forming, in vivo, not only polymeric forms, but also a monomer in which a single disulfide bond links the C-terminal domain to the N-terminal domain, replacing the two intermolecular disulfide bonds normally formed by the corresponding cysteine side chains. No such monomeric subunits have been observed in normal wheat lines, only polymeric forms. NCAFM of the native, unreduced 93 K monomer showed fibrils of varying lengths but a length of about 110 nm was particularly noticeable whereas the reduced form showed rod-like structures with a length of about 300 nm or greater. The 110 nm fibrils may represent the length of the disulfide-linked monomer, in which case they would not be in accord with the beta-spiral model, but would favor a more extended conformation for the polypeptide chain, possibly polyproline II.     

Inhibition of murine N1-acetylated polyamine oxidase by an acetylenic amine and the allenic amine, MDL 72527

Murine N¹-acetylated polyamine oxidase (mPAO) was treated with N,N′-bis-(prop-2-ynyl)-1,4-diaminobutane, a poor substrate and inhibitor for the enzyme, with K_m and K_i values in the millimolar range. Apparently, its oxidation produces prop-2-ynal, which reacts with amino acyl nucleophiles. Using a steady-state kinetic assay, four phases were identified, the first being the oxidation of the compound via Michealis-Menten-type kinetics. As prop-2-ynal accumulates, there is a biphasic reduction in the rate. This process leads to an mPAO form that is nearly inactive (fourth phase), but displays classical Michealis-Menten-type kinetics. The enzyme-bound flavin is not modified in this process. In contrast, micromolar concentrations of the MDL 72527 (N,N′-bis-[buta-2,3-dienyl]-1,4-diaminobutane) inhibited mPAO rapidly and completely. It inhibits by first binding tightly and apparently irreversibly, and then slowly converts to a species where the inhibitor is covalently bound to the N5-position of the flavin’s isoalloxazine ring. The covalent adduct was identified as a flavocyanine.     

p-Cresol methylhydroxylase: alteration of the structure of the flavoprotein subunit upon its binding to the cytochrome subunit

The structures of two forms of a recombinant flavoprotein have been determined at high resolution and compared. These proteins are (1) the flavocytochrome c p-cresol methylhydroxylase (rPCMH, 1.85 A resolution) and (2) the cytochrome-free flavoprotein subunit of rPCMH (PchF, 1.30 A resolution). A significant conformational difference is observed in a protein segment that is in contact with the re face of the isoalloxazine ring of FAD when the structure of PchF is compared to the subunit in the intact flavocytochrome. This structural change is important for optimum catalytic function of the flavoprotein, which has been shown to be dependent on the presence of the cytochrome subunit. This change results in different protein-flavin and apparently different protein-substrate interactions that have a "tuning effect" on the electronic and redox properties of bound p-cresol and the covalently bound FAD. The conformational change in the segment in the cofactor-binding site is induced by a small rearrangement in the flavoprotein-cytochrome interface region of the flavoprotein.     

一种采集稻纵卷叶螟卵的高效简便新方法

研究了稻纵卷叶螟Cnaphalocrocis medinalis Guenee的"烧杯+湿纱布"卵收集方法,并与"塑料袋收集法"和"塑料杯收集法"进行了比较。结果表明,"烧杯+湿纱布"卵收集法能显著地延长了稻纵卷叶螟成蛾的寿命、提高单雌产卵量和卵孵化率。采用此法收集稻纵卷叶螟的卵更高效和简便。