电离辐射对蛋白质分子作用的量子孤波分析

本文运用量子力学方法对蛋白质分子中孤波传播的非线性动力学特征进行了探讨。研究表明：电离辐射产生的自由基对蛋白质分子的伤害将会对携带能量、信息的孤立子波传播产生较为显著的影响。     

The Analysis of Homegarden Plant Diversity at the Mongolian Household Level in Horqin Sandy Land——A Case Study in Horqin Left Wing Real Banner of Inner Mongolia

In order to show the function of agricultural protection of biodiversity at the level of homegarden, comparative analysis was operated between 20 farmer households through random sampling and farmers recommended in Horqin Left Wing Real Banner Tongliao city Inner Mongolia Autonomous Region and 10 farmer households of the Han nationality in the neighborhood. Thirty sampling plots of homegardens are investigated. The result indicates there are 100 homegarden plants in Bashou village, 84 in Hariwusu livestock farm, and 87 in Gongjihao village. It involves the cultivated and wild vegetables, fruits, and plants used for ornamental, medicine and forage. The comparative analysis of species richness demonstrates, among these villages the species richness of Bashou village is highest, while Hariwusu livestock farm is lowest. The former village contributes greatly to the conservation of plant biodiversity. These differences result from their respectively different natural conditions, homegarden areas, homegarden history, homegarden management conditions, traditional knowledge and culture understanding of homegarden manager, local farmers and herdsmen on homegarden plants.The homegarden plants from the three investigated villages are cataloged preliminary in the end of the paper.     

冷却肉糜辐照脂肪氧化影响因素与抑制效果研究

研究了低剂量辐照、抗氧化剂、包装条件以及贮藏时间等影响辐照冷却肉脂肪氧化的主要因素和抑制效果.结果表明,对冷却猪肉的辐照处理会加剧其脂肪的氧化,真空包装或无氧包装可以明显的降低贮藏期间辐照冷却肉的脂肪氧化.辐照处理前在肉糜中添加抗氧化剂并采用真空包装时, 肉糜可以贮藏2周以上而不产生脂肪氧化.比较几种主要的抗氧化剂的效果表明,茶多酚在抑制辐照冷却肉糜脂肪氧化方面效果最好.     

BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype

Immunologically intact BALB/c mice infected with Leishmania mexicana develop non-healing progressively growing lesions associated with a biased Th2 response while similarly infected IL-4Rα-deficient mice fail to develop lesions and develop a robust Th1 response. In order to determine the functional target(s) for IL-4/IL-13 inducing non-healing disease, the course of L. mexicana infection was monitored in mice lacking IL-4Rα expression in specific cellular compartments. A deficiency of IL-4Rα expression on macrophages/neutrophils (in LysM(cre)IL-4Rα(-/lox) animals) had minimal effect on the outcome of L. mexicana infection compared with control (IL-4Rα(-/flox)) mice. In contrast, CD4(+) T cell specific (Lck(cre)IL-4Rα(-/lox)) IL-4Rα(-/-) mice infected with L. mexicana developed small lesions, which subsequently healed in female mice, but persisted in adult male mice. While a strong Th1 response was manifest in both male and female CD4(+) T cell specific IL-4Rα(-/-) mice infected with L. mexicana, induction of IL-4 was manifest in males but not females, independently of CD4(+) T cell IL-4 responsiveness. Similar results were obtained using pan-T cell specific (iLck(cre)IL-4Rα(-/lox)) IL-4Rα(-/-) mice. Collectively these data demonstrate that upon infection with L. mexicana, initial lesion growth in BALB/c mice is dependent on non-T cell population(s) responsive to IL-4/IL-13 while progressive infection is dependent on CD4(+) T cells responsive to IL-4.     

Pleuston communities are buffered from regional flood pulses: the example of ostracods in the Paraná River floodplain, Brazil

1. It is widely acknowledged that sudden, large‐scale flood pulses are drivers of benthic and planktonic biodiversity change in floodplains. The impact of such pulses on pleuston (biotic communities associated with root systems of floating plants) remains to be demonstrated. Here, we investigate the effects of local and regional drivers on seasonal changes in abundance and diversity of ostracod communities in pleuston. 2. Temporal and spatial distribution patterns of species richness, abundance, diversity and evenness of ostracods associated with the floating water hyacinth, Eichhornia crassipes, in a lentic environment from the upper Paraná River floodplain, were investigated in relation to local, as well as regional, environmental factors. Ostracods were sampled monthly over an annual cycle (March 2004–February 2005). Twenty‐seven species were found, representing the families Cyprididae, Candonidae, Limnocytheridae and Darwinulidae. Both diversity and abundance of ostracod communities showed seasonal changes, although species turn‐over during the year was limited. 3. We tested two hypotheses concerning the causality of these fluctuations: seasonal recruitment and influx of allochthonous ostracods during the flood pulse. Our results indicate that seasonal recruitment is more likely to be the driver of fluctuations in relation to the flood pulse. We postulate that pleuston communities are buffered against possible detrimental effects of flood pulses.     

大豆疫霉菌的EMS化学诱变

以甲基磺酸乙酯(ethylmethane sulfonate,EMS)为诱变剂,通过其对大豆疫霉菌Phytophthora sojae休止孢萌发的影响,确定化学诱变条件。通过收集单卵孢子,建立了包含640个单卵孢子系的突变体库,其中约有50%的诱变菌系在培养性状和菌落形态方面发生了明显变化,菌落形态多样,表现出较紧密或松散,近圆形或不规则;气生菌丝减少,生长速度较慢或快;在卵孢子产量方面,8.13%的菌系有增加,20.41%的菌系减少,27.82%的菌系极少或者没有卵孢子产生,43.64%的菌系卵孢子产量类似野生型。以质膜氢离子泵蛋白基因PsPMA1(plasma membrane H+-ATPase1)为对象,通过TILLING技术,从320个大豆疫霉菌突变体中获得9个突变体,进一步确认了EMS对大豆疫霉菌的诱变效果,并且估算EMS对大豆疫霉菌的诱变频率至多每115kb发生一个核苷酸变异。新构建的突变体库为开展大豆疫霉病菌的功能基因组研究奠定了遗传材料基础。     

An investigation of dye plants and their colourant substances in the eastern Mediterranean region of Turkey

Plants traditionally used to dye carpets and woven matting in the eastern Mediterranean region were investigated. The survey was carried out in Kadirli, Feke, Aladağ, the surrounding villages of Adana, and in Osmaniye (Turkey). According to the survey results, 37 species of plants belonging to 29 families were used in natural dye production. The total dye contents of collected plants ranged from 1.5% to 10.0%. Mordants and dyes obtained from some of these plants were used to treat wool yarn and the yarn's dyed properties were tested. Some of the plants were also examined in terms of cultivation and fifteen were transferred to a Dye Plants Collection Garden. They were Alnus glutinosa (L.) Gaertn. ssp. antitaurica Yalt., Arctium minus ssp. pubens (Bab.) Arènes, Chrysanthemum segetum L., Cistus creticus L., Cotinus coggygria Scop., Datisca cannabina L., Galium verum ssp. glabrescens Ehrend., Isatis tinctoria ssp. tomentella (Boiss. & Balansa) P.H.Davis, Melissa officinalis ssp. inodora Bornm., Mentha longifolia (L.) Huds. ssp. longifolia , Reseda lutea var. lutea L., Rhamnus oleoides ssp. graecus (Boiss. & Reut.) Holmboe, Rhus coriaria L., Rubia tinctorum L., and Rumex patientia L.  © 2004 The Linnean Society of London, Botanical Journal of the Linnean Society , 2004, 146 , 71–77.     

The oligomerization of a family of four genetically clustered human gastrointestinal mucins

Mucins are synthesized and secreted by many epithelia. They are complex glycoproteins that offer cytoprotection. In their functional configuration, mucins form oligomers by a biosynthetic process that is poorly understood. A family of four human gastrointestinal mucin genes (MUC2, MUC5AC, MUC5B, and MUC6) is clustered to chromosome 11p15.5. To study oligomerization of these related mucins, we performed metabolic labeling experiments with [35S]amino acids in LS174T cells, and isolated mucin precursors by specific immunoprecipitations that were analyzed on SDS-PAGE. Each of the precursors of MUC2, MUC5AC, MUC5B, and MUC6 formed a single species of disulfide-linked homo-oligomer within 1 h after pulse labeling. Based on apparent molecular masses, these oligomeric precursors were most likely dimers. Inhibition of vesicular RER-to-Golgi transport, with brefeldin A and CCCP, did not affect the dimerization of MUC2 precursors, localizing dimerization to the RER. O-Glycosylation of MUC2 followed dimerization. Inhibition of N- glycosylation by tunicamycin retarded, but did not inhibit, dimerization, indicating that N-glycans play a role in efficient dimerization of MUC2 precursors. Based on sequence homology, the ability of MUC2, MUC5AC, MUC5B and MUC6 to dimerize most likely resides in their C-terminal domains. Thus, the RER-localized dimerization of secretory mucins likely proceeds by similar mechanisms, which is an essential step in the formation of the human gastrointestinal mucus- gels.      

沙柳灌丛植株形态与气流结构野外观测研究

通过野外观测,研究了毛乌素沙地南缘平沙地人工栽种单行沙柳灌丛的植株形态与气流结构.结果表明,沙柳灌能降低迎风侧和背风侧的近地面风速,使沙粒在植株下部沉积.积沙体积(V₂)与植株体积(V₁)相关性较高(R=0.696).单行沙柳灌丛在4 m高处风速为6 m·s^-1时的气流结构特征为:迎风侧3H处风速已降低,2 H处风速又得到加强,之后在背风侧迅速降低,1 H处达到最低值,然后逐渐恢复到旷野风速.防护距离约为17H',有效防护距离约为13 H'.沙柳灌丛对植株高度以上高度处风速影响较小.