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991.
992.
Antonow-Schlorke I Ebert M Li C Gschanes A Witte OW McDonald T Nathanielsz PW Schwab M 《Journal of medical primatology》2007,36(1):17-20
BACKGROUND: Maternal antenatal glucocorticoid therapy is used to accelerate lung maturation of immature babies at risk of preterm delivery. It acutely affects brain activity of the human fetus and reduces the immunoreactivity of neurocytoskeletal and synaptic proteins in the fetal baboon brain. These effects might be based on cerebral energy failure due to a decreased neuronal glucose uptake that has been shown in vitro. METHODS: Glucose uptake into the brain is selectively facilitated by GLUT1 expressed in the blood-brain barrier and GLUT3 expressed in the neuronal membrane. Immunohistochemical distribution of GLUT1 and GLUT3 were examined in the frontal neocortex of the fetal baboon brain at 0.73 gestation (i.e. similar to 28 weeks of human gestation) after maternal betamethasone administration, mimicking the clinical dose regimen. RESULTS: Betamethasone did not alter GLUT1 and GLUT3 immunoreactivity. CONCLUSIONS: The results suggest that inhibition of glucose uptake is not the mechanism for the cerebral effects of antenatal glucocorticoids. 相似文献
993.
994.
Druery GV Shimmin GA Taggart DA Temple-Smith PD Breed WG McDonald CH Finlayson GR Paris MC 《Animal reproduction science》2007,99(3-4):363-376
This study investigates the effect of three exogenous gonadotrophin regimens on ovarian follicular development in southern hairy-nosed wombats during the non-breeding season. Females were given either porcine follicle stimulating hormone (pFSH; total of 200 mg at 12 h intervals over 7 (Group 1), or 4 days (Group 2)), or pregnant mares' serum gonadotrophin (PMSG; single dose of 150 I.U. (Group 3)). In all treatment groups 25 mg of porcine luteinising hormone (pLH) was used to trigger maturation; Groups 1 and 2 received pLH 12 h after the final pFSH injection and Group 3 received pLH 72 h after PMSG. The results showed Group 1 produced significantly more follicles per ovary (5.91+/-1.28) than Group 2 (1.67+/-0.62), or Group 3 (2.17+/-1.16) at p<0.05. Control females received saline injections concurrently with the three treatment groups (n=6; 2 control animals for each treatment group). No follicular development occurred in any control female. Analysis of oocyte nuclear status revealed that while oocytes from all three treatment groups had resumed meiosis, only those in Group 1 (7-day pFSH/pLH treatment) progressed to metaphase II. These results have implications for the development of assisted breeding strategies in this species. 相似文献
995.
Terry Done Emre Turak Mary Wakeford Lyndon DeVantier Abbi McDonald David Fisk 《Coral reefs (Online)》2007,26(4):789-805
Coral communities were monitored at Pandora Reef, nearshore Great Barrier Reef from 1981 to 2005 using photography and videography.
In the 1980s, regional elevation of land-based nutrients in coastal waters (ca. 2–6 times pre-European levels of early 1800s)
did not prevent overall recovery of coral cover and diversity following a sequence of environmental disturbances in the 1970s.
However, prospects for a repeat of such resilience following catastrophic mortality from high-temperature bleaching in 1998
and a cyclone in 2000 are not clear. Different coral communities around the reef varied greatly in relation to impacts and
recovery. Fore-reef communities dominated by acroporids (fast growing branching and tabular Acropora and foliose Montipora) recovered strongly in the 1980s following apparently severe impacts by cyclone, flood and heat wave disturbances in the
1970s, attaining 60–90% cover by stabilizing rubble and outgrowing macro-algae in <10 years. In the back-reef, by contrast,
poritid-dominated communities (massive and finger Porites and columnar Goniopora and Alveopora) had more stable trajectories and smaller impact from recent disturbances: recovery was well underway in 2005. The contrasting
trajectories of different parts of the reef reflect differential survival of more persistent versus more ephemeral taxa, notably
poritids and acroporids, respectively, both major contributors to framework and cover on reefs globally. A repeat of earlier
resilience appears possible in the shallow fore-reef, but unlikely in the deeper fore-reef, which had few viable fragments
or recruits in 2005. The main limits on recovery may be (1) reduced supply of coral larvae due to widespread regional losses
of coral brood stock and (2) the reduced intervals between disturbances associated with global climate change. The presence
of a high abundance of Acroporidae is a major pre-disposing risk factor for climate change impacts. 相似文献
996.
The distance decay of similarity in ecological communities 总被引:11,自引:0,他引:11
Biological similartiy typically decreases with geographical distance. Despite the recent attention to the distance decay relationship, there is no consensus on how the relationship varies across organism groups, geographic gradients and environments. We first conducted a quantitative meta-analysis of 401 distance decay relationships across a wide range of organisms, ecosystems and geographical gradients, and then united the effects of categorical and continuous variables on the rate of distance decay using a general linear model (GLM). As effect sizes we used the similarity at one km distance (initial similarity) and the distance that halves the similarity from its value at one km distance (halving distance). Both the initial similarity and halving distance were significantly affected by variables characterizing the spatial scale, organism properties, study region and ecosystem concerned. The patterns appear robust as the results of meta-analysis and GLM only differed in marginal details. According to GLM with Akaike's information criterion, the most parsimonious models explained 55.3 and 37.6% of variance in initial similarity and halving distance, respectively. Across large scales, similarity was decreasing slightly faster at high latitudes than at low latitudes, while small-scale turnover was higher at low latitudes. We also found significant differences in initial similarity among the realms, with terrestrial systems showing higher small-scale beta diversity. The decrease in community similarity at large scales was higher among organisms that are actively mobile than among passively dispersed organisms. We conclude that regression of similarity against distance unites several ecological phenomena such as dispersal propensity and environmental structuring, and provides an effective approach for gauging the spatial turnover across sites. We also found that the patterns in beta-diversity are highly scale-dependent. 相似文献
997.
998.
The origins of pathogens and their past and present migration patterns are often unknown. We used phylogenetic haplotype clustering in conjunction with model-based coalescent approaches to reconstruct the genetic history of the barley leaf pathogen Rhynchosporium secalis using the avirulence gene NIP1 and its flanking regions. Our results falsify the hypothesis that R. secalis emerged in association with its host during the domestication of barley 10,000 to 15,000 years ago in the Fertile Crescent and was introduced into Europe through the migration of Neolithic farmers. Estimates of time since most recent common ancestor (2500-5000 BP) placed the emergence of R. secalis clearly after the domestication of barley. We propose that modern populations of R. secalis originated in northern Europe following a host switch, most probably from a wild grass onto cultivated barley shortly after barley was introduced into northern Europe. R. secalis subsequently spread southwards into already established European barley-growing areas. 相似文献
999.
McDonald MD Gilmour KM Barimo JF Frezza PE Walsh PJ Perry SF 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,146(3):366-374
The neurochemical, serotonin (5-hydroxytryptamine; 5-HT) is involved in the regulation of toadfish pulsatile urea excretion as well as the teleost hypoxia response. Thus, the goal of this study was to determine whether environmental conditions that activate branchial chemoreceptors also trigger pulsatile urea excretion in toadfish, since environmental dissolved oxygen levels in a typical toadfish habitat show significant diel fluctuations, often reaching hypoxic conditions at dawn. Toadfish were fitted with arterial, venous and/or buccal catheters and were exposed to various environmental conditions, and/or injected with the O(2) chemoreceptor agonist NaCN or the 5-HT(2) receptor agonist alpha-methyl-5HT. Arterial PO(2), as well as ammonia and urea excretion were monitored. Natural fluctuations in arterial PO(2) levels in toadfish did not correlate with the occurrence of a urea pulse. Chronic exposure (24 h) of toadfish to hyperoxia was without effect on nitrogen excretion, however, exposure to hypoxia caused a significant reduction in the frequency of urea pulses, and exposure to hypercapnia resulted in a reduction in the percentage of nitrogen waste excreted as urea. Of toadfish exposed acutely to hypoxia, 20% pulsed within 1 h, whereas none pulsed after normoxic or hypercapnic treatments. Furthermore, 20% of fish injected intravenously with NaCN pulsed within 1 h of injection, but no fish pulsed after injection of NaCN into the buccal cavity. To test whether environmental conditions affected 5-HT(2) receptors, toadfish were injected with alpha-methyl-5HT, which elicits urea pulses in toadfish. No significant differences in pulse size occurred among the various environmental treatments. Our findings suggest that neither the environmental conditions of hypoxia, hyperoxia or hypercapnia, nor direct branchial chemoreceptor activation by NaCN play a major role in the regulation of pulsatile urea excretion in toadfish. 相似文献
1000.
BACKGROUND: Increases in DNA transfection efficiencies for non-viral vectors can be achieved through rational design of novel cationic building blocks. Based on previous results examining DNA condensation by polyamines, novel gemini surfactants have been designed that incorporate aza or imino substituents within the spacer group in order to increase interactions with DNA and potentially improve their DNA transfection ability. METHODS: Transfection efficiencies and cell toxicity of gemini nanoparticles constructed from plasmid DNA, gemini surfactant, and a neutral lipid were measured in COS7 cells using a luciferase assay. Structural properties of nanoparticles were examined by using circular dichroism, particle size, zeta potential, and small-angle X-ray scattering (SAXS) measurements. RESULTS: The incorporation of aza and imino substituents within the spacer group was observed to enhance the transfection ability of gemini surfactants. Incorporation of an imino group in the structure of the 1,9-bis(dodecyl)-1,1,9,9-tetramethyl-5-imino-1,9-nonanediammonium dibromide surfactant (12-7NH-12) resulted in a statistically significant (p < 0.01) 9-fold increase in transfection compared to an unsubstituted gemini surfactant and a 3-fold increase compared to the corresponding aza-substituted compound. A pH-dependent transition in size and zeta potential was observed to occur at pH 5.5 for complexes formed from the 12-7NH-12 compound. SAXS results show weakly ordered structures and the presence of multiple phases. CONCLUSIONS: The incorporation of a pH-active imino group within the spacer of the gemini surfactant results in a significant increase in transfection efficiency that can be related to both pH-induced changes in nanoparticle structure and the formation of multiple phases that more readily allow for membrane fusion that may facilitate DNA release. 相似文献