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981.
982.
Allelopathy of bacteria in a lattice population: Competition between colicin-sensitive and colicin-producing strains 总被引:10,自引:0,他引:10
We analyse the population dynamics of two strains of bacteria (Escherichia coli): one strain produces a toxin (called colicin) that increases the mortality of a colicin-sensitive strain in the neighbourhood, but does not harm the colicin-producing strain itself. On the other hand, in the absence of colicin in the environment, the colicin-sensitive strain enjoys a higher population growth rate. The model is closely related to the evolutionary dynamics of social interaction. It has been established previously that a perfectly mixing population shows bistability; that is, whichever strain dominates initially tends to defeat the other. On the other hand, empirical and computer simulation results in lattice structured populations show neither co-existence nor bistability of the two strains. In this paper, we analyse the lattice model based on pair approximation (forming a system of ordinary differential equations of global densities and local densities), and by the direct c omputer simulation of the spatial stochastic model. Both the pair approximation dynamics and the computer simulation show that, for most regions of parameter values, one strain defeats the other, irrespective of the initial abundance. However, the pair approximation analysis also suggests a relatively narrow parameter region of bistability, which should disappear when the model is considered on a lattice of infinitely large size. The biological implications of the results and the relationship of the present model with other models of the evolution of spite or altruistic behaviours are discussed. This suggests that the dynamics reflected by the spatially explicit lattice model may be sufficient, perhaps even necessary, to support the evolution of colicin. 相似文献
983.
984.
Contribution of the Disulfide Bond of the A Subunit to the Action of Escherichia coli Heat-Labile Enterotoxin
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Keinosuke Okamoto Tomohiko Nomura Yoshio Fujii Hiroyasu Yamanaka 《Journal of bacteriology》1998,180(6):1368-1374
Escherichia coli heat-labile enterotoxin (LT) consists of an A subunit and five B subunits. These subunits oligomerize into an assembled holotoxin within the periplasm. Structural analysis of LT has revealed that the A subunit interacts with the B subunit through its carboxy terminus. This indicates that the carboxy-terminal portion of the protein is required for assembly of holotoxin in the periplasm. However, it is not known whether other regions of the A subunit contribute to the assembly. The A subunit constituting the holotoxin contains a disulfide bond between Cys-187 and Cys-199. It has been observed in many proteins that the intramolecular disulfide bond is deeply involved in the function and tertiary structure of the protein. We speculated that the disulfide bond of the A subunit contributes to the assembly in the periplasm, although the bond is not a structural element of the carboxy-terminal portion of the A subunit. We replaced these cysteine residues of the A subunit by oligonucleotide-directed site-specific mutagenesis and analyzed the LTs produced by cells containing the mutant LT genes. The amount of the mutant holotoxin produced was small compared with that of the wild-type strain, indicating that the disulfide bond of the A subunit contributes to the structure which functions as the site of nucleation in the assembly. A reconstitution experiment in vitro supported the notion. Subsequently, we found that the mutant A subunit constituting holotoxin is easily degraded by trypsin and that in cells incubated with mutant LTs, the lag until the intracellular cyclic AMP begins to accumulate is longer than in cells incubated with native LTs. These results might be useful for the analysis of the interaction of LT with target cells at the molecular level. 相似文献
985.
Kaori Tomita-Yokotani Yoshiharu Fujii Hirofumi Hashimoto Masamichi Yamashita 《Biological Sciences in Space》2003,17(1):14-17
Allelopathy between Mucuna pruriens (velvet bean) and Lactuca sativa (lettuce) was studied under 3D-clinorotation. Growth of both roots and shoots of lettuce seedlings was suppressed by the presence of velvet bean. The degree of suppression was less on the clinostat compared to the normal static earth gravity. L-DOPA (L-3, 4-dihydroxyphenylalanine) is known to be a major substance in allelopathy of velvet bean. Amount of L-DOPA diffused out from a sintered filter paper into agar medium was compared between clinorotation and control group, and found no significant difference. It was concluded that some factors related to release, transport, and sensing phenomena of allelopathic substances may be responsible to the new findings in this study. 相似文献
986.
Minobu Shimizu Keita Suzuki Nobuharu Fujii Hideyuki Takahashi 《Biological Sciences in Space》2003,17(3):232-233
Cucumber seedlings have potential to develop two pegs on the transition zone between the hypocotyl and root. Seedlings grown in a horizontal position suppress the development of the peg on the upper side of the transition zone in response to gravity. To understand how the response to gravity suppresses peg formation, we screened cucumber mRNAs to identify the mRNA in the non-peg side that accumulates more than in the peg side. For screening, we determined conditions of fluorescent differential display (FDD). Then, we carried out FDD and found 4 cDNA bands that repeatedly showed stronger intensity in the non-peg side than the peg side. We isolated one of these RT-PCR products. Northern blotting showed the pattern of its mRNA accumulations corresponding to the results of FDD. 相似文献
987.
988.
The mode of phospholipase C activation in platelet cells induced by didecanoyl (C10)-phosphatidic acid (PA) was investigated with washed rabbit platelets. The C10-PA dose-dependently induced aggregation and serotonin secretion, as well as increases in cytoplasmic free Ca2+ concentration and 1,2-diacylglycerol formation. None of these responses was evoked unless Ca2+ had been added to the platelet suspension. Furthermore, under the conditions of various intracellular Ca2+ concentrations which were set by treatment of the cells with ionomycin and Ca2+, C10-PA promoted 1,2-diacylglycerol formation only at the Ca2+ concentration of 300 nM or higher, whereas thrombin induced the formation even at 100 nM Ca2+. These results suggest that PA activates platelet phospholipase C in cooperation with Ca2+ and contributes to platelet activation through such an effect. 相似文献
989.
A novel biochemical assay system for detecting the early stage of flowering is reported. Peroxidase isozymes in shoot apices of Pharbitis nil plants that had been exposed to flower‐inducing or non‐inducing conditions were analyzed by isoelectric focusing in polyacrylamide gels and activity staining for peroxidase. Several isozymes with pH 8.5–8.8 appeared for the first time 7 days after the beginning of short‐day treatment, but not after nightbreak (non‐inducing) treatment. When shoot tips were cultured in vitro, these same isozymes also appeared after short‐day treatment but not after night‐break treatment. The extent of the appearance of these isozymes was reduced by exposure to high or low temperature during the inductive dark period and removal of cotyledons after the inductive dark period. Such treatments also reduced the extent of flowering. The appearance of an isozyme with pH 8.5 was more closely correlated with flowering than that of the other isozymes. From these results, the appearance of this peroxidase isozyme in shoot apices is discussed as a biochemical marker of flowering in intact plants and in cultured shoot tips. 相似文献
990.
Toshihiro Fujioka Miyako Yamamoto Yoshiki Kashiwada Hiroko Fujii Kunihide Mihashi Yasumasa Ikeshiro Ih-Sheng Chen Kuo-Hsiung Lee 《Bioorganic & medicinal chemistry letters》1998,8(24):1184-3482
Three novel diterpenes, dysokusones A (1), B (2), and C (3), were isolated from the stem of Dysoxylum kuskusense as cytotoxic substances. The structures were established by spectroscopic examinations. Compounds 1, 2, and 3 were cytotoxic toward HL-60(TB) cells with EC50 values of 2.25, 6.35, and 2.37 μM, respectively. Compound 1 also displayed cytotoxicity against K-562 and NCI-H522 cells with EC50 values of 5.04 and 4.80 μM, respectively. 相似文献