全文获取类型
收费全文 | 361篇 |
免费 | 26篇 |
专业分类
387篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 16篇 |
2020年 | 7篇 |
2019年 | 16篇 |
2018年 | 16篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 22篇 |
2014年 | 15篇 |
2013年 | 18篇 |
2012年 | 23篇 |
2011年 | 30篇 |
2010年 | 18篇 |
2009年 | 36篇 |
2008年 | 14篇 |
2007年 | 18篇 |
2006年 | 12篇 |
2005年 | 12篇 |
2004年 | 15篇 |
2003年 | 15篇 |
2002年 | 12篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1998年 | 3篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
排序方式: 共有387条查询结果,搜索用时 15 毫秒
61.
Morales Pedraza J 《Cell and tissue banking》2008,9(4):317-322
The main purpose of the International Atomic Energy Agency (IAEA) Public Awareness Strategies for Tissue Banks is to provide
guidance on organizing and running awareness campaigns, in order to consolidate tissue banking activities. Within the IAEA
Public Awareness Strategies for Tissue Banks, there are two important topics, which need to be singled out due to their importance
for a successful public and professional awareness campaign. These are the selection of the audiences and the organization
of media events within a Communication Strategy. The experience in the field of tissue banking in several countries has shown
that interaction between the public, the professional health care staff, the media and the tissue bank personnel is essential
if the activities of the banks are to be successful. It must be emphasized however, that any public and professional awareness
strategy will not be successful, unless it is considered as part of an integrated system that is adopted by the concerned
Government. 相似文献
62.
63.
64.
Morales Pedraza J Sánchez Noda EO Rodríguez Cardona RL Otero I 《Cell and tissue banking》2009,10(2):149-152
The first multi-tissue bank was founded at Havana in 1958. At that time, freeze-drying was used at the bank as a method of
preserving, as well as Cobalt 60 irradiation to sterilise bone tissue, heart valves and others. The impact of the IAEA program
in tissue banking activities in Cuba can be summarised as follows: (a) Increase in the production of sterilised tissues using
ionising radiation (bone, pig skin and amnion) for medical treatment in the tissue bank of the Hospital Frank Pais; (b) increase
of the quality of the productions of bone tissues, pig skin and amnion; (c) reduction in the import of tissues by increasing
the local production of tissues; (d) sustainability in the number of donors through the implementation of a public and professional
awareness campaign; (e) training of six persons in the Regional Training Centre of Buenos Aires; (f) qualification of one
person in the administration of a tissue bank and in the implementation of a Quality System. The amount of tissues produced
and sterilised using the ionising radiation techniques in the established banks was 25,510 units. The amount of patients treated
with sterilised tissues produced by the established banks was 2,448. 相似文献
65.
Until 2000, efforts into organising tissue banks in Brazil had not progressed far beyond small “in house” tissue storage repositories,
usually annexed to Orthopaedic Surgery Services. Despite the professional entrepreneurship of those working as part time tissue
bankers in such operations, best practices in tissue banking were not always followed due to the lack of regulatory standards,
specialised training, adequate facilities and dedicated personnel. The Skin Bank of the Plastic Surgery Department of the
Hospital das Clinicas of Sao Paulo, the single skin bank in Brazil, was not an exception. Since 1956, restricted and unpredictable
amounts of skin allografts were stored under refrigeration for short periods under very limited quality controls. As in most
“tissue banks” at that time in Brazil, medical and nursing staff worked on a volunteer and informal basis undergoing no specific
training. IAEA supported the implementation of the tissue banking program in Brazil through the regional project RLA/7/009
“Quality system for the production of irradiated sterilised grafts” (1998–2000) and through two interregional projects INT/6/049 “Interregional Centre of Excellence in Tissue Banking”, during the period 2002–2004 and INT/6/052 “Improving the Quality of Production and Uses of Radiation Sterilised Tissue Grafts”, during the period 2002–2004. In 2001–2002, the first two years of operation of the HC-Tissue Bank, 53 skin transplants
were carried out instead of the previous 4–5 a year. During this period, 75 individuals donated skin tissue, generating approximately
90,000 cm2 of skin graft. The IAEA program were of great benefit to Brazilian tissue banking which has evolved from scattered make shift
small operations to a well-established, high quality tissue banking scenario. 相似文献
66.
67.
Battula S Hao S Pedraza PL Stier CT Ferreri NR 《American journal of physiology. Renal physiology》2011,301(1):F94-100
The effects of TNF gene deletion on renal Na(+)-K(+)-2Cl(-) cotransporter (NKCC2) expression and activity were determined. Outer medulla from TNF(-/-) mice exhibited a twofold increase in total NKCC2 protein expression compared with wild-type (WT) mice. This increase was not observed in TNF(-/-) mice treated with recombinant human TNF (hTNF) for 7 days. Administration of hTNF had no effect on total NKCC2 expression in WT mice. A fourfold increase in NKCC2A mRNA accumulation was observed in outer medulla from TNF(-/-) compared with WT mice; NKCC2F and NKCC2B mRNA accumulation was similar between genotypes. The increase in NKCC2A mRNA accumulation was attenuated when TNF(-/-) mice were treated with hTNF. Bumetanide-sensitive O(2) consumption, an in vitro correlate of NKCC2 activity, was 2.8 ± 0.2 nmol·min(-1)·mg(-1) in medullary thick ascending limb tubules from WT, representing ~40% of total O(2) consumption, whereas, in medullary thick ascending limb tubules from TNF(-/-) mice, it was 5.6 ± 0.3 nmol·min(-1)·mg(-1), representing ~60% of total O(2) consumption. Administration of hTNF to TNF(-/-) mice restored the bumetanide-sensitive component to ~30% of total O(2) consumption. Ambient urine osmolality was higher in TNF(-/-) compared with WT mice (2,072 ± 104 vs. 1,696 ± 153 mosmol/kgH(2)O, P < 0.05). The diluting ability of the kidney, assessed by measuring urine osmolality before and after 1 h of water loading also was greater in TNF(-/-) compared with WT mice (174 ± 38 and 465 ± 81 mosmol/kgH(2)O, respectively, P < 0.01). Collectively, these findings suggest that TNF plays a role as an endogenous inhibitor of NKCC2 expression and function. 相似文献
68.
Solis M Nakhaei P Jalalirad M Lacoste J Douville R Arguello M Zhao T Laughrea M Wainberg MA Hiscott J 《Journal of virology》2011,85(3):1224-1236
The rapid induction of type I interferon (IFN) is essential for establishing innate antiviral responses. During infection, cytoplasmic viral RNA is sensed by two DExD/H box RNA helicases, RIG-I and MDA5, ultimately driving IFN production. Here, we demonstrate that purified genomic RNA from HIV-1 induces a RIG-I-dependent type I IFN response. Both the dimeric and monomeric forms of HIV-1 were sensed by RIG-I, but not MDA5, with monomeric RNA, usually found in defective HIV-1 particles, acting as a better inducer of IFN than dimeric RNA. However, despite the presence of HIV-1 RNA in the de novo infection of monocyte-derived macrophages, HIV-1 replication did not lead to a substantial induction of IFN signaling. We demonstrate the existence of an evasion mechanism based on the inhibition of the RIG-I sensor through the action of the HIV-1 protease (PR). Indeed, the ectopic expression of PR resulted in the inhibition of IFN regulatory factor 3 (IRF-3) phosphorylation and decreased expression of IFN and interferon-stimulated genes. A downregulation of cytoplasmic RIG-I levels occurred in cells undergoing a single-cycle infection with wild-type provirus BH10 but not in cells transfected with a protease-deficient provirus, BH10-PR(-). Cellular fractionation and confocal microscopy studies revealed that RIG-I translocated from the cytosol to an insoluble fraction during the de novo HIV-1 infection of monocyte-derived macrophages, in the presence of PR. The loss of cytoplasmic RIG-I was prevented by the lysosomal inhibitor E64, suggesting that PR targets RIG-I to the lysosomes. This study reveals a novel PR-dependent mechanism employed by HIV-1 to counteract the early IFN response to viral RNA in infected cells. 相似文献
69.
David J. Mendoza-Aguayo Héctor M. Poggi-Varaldo Jaime García-Mena Ana C. Ramos-Valdivia Luis M. Salgado Mayra de la Torre-Martínez Teresa Ponce-Noyola 《Archives of microbiology》2014,196(1):25-33
The catalytic fraction of the Cellulomonas flavigena PN-120 oligomeric β-glucosidase (BGLA) was expressed both intra- and extracellularly in a recombinant diploid of Saccharomyces cerevisiae, under limited nutrient conditions. The recombinant enzyme (BGLA15) expressed in the supernatant of a rich medium showed 582 IU/L and 99.4 IU/g dry cell, with p-nitrophenyl-β-d-glucopyranoside as substrate. BGLA15 displayed activity against cello-oligosaccharides with 2–5 glucose monomers, demonstrating that the protein is not specific for cellobiose and that the oligomeric structure is not essential for β-d-1,4-bond hydrolysis. Native β-glucosidase is inhibited almost completely at 160 mM glucose, thus limiting cellobiose hydrolysis. At 200 mM glucose concentration, BGLA15 retained more than 50 % of its maximal activity, and even at 500 mM glucose concentration, more than 30 % of its activity was preserved. Due to these characteristics of BGLA15 activity, recombinant S. cerevisiae is able to utilize cellulosic materials (cello-oligosaccharides) to produce bioethanol. 相似文献
70.
Carlos E. Pedraza Christopher Taylor Albertina Pereira Michelle Seng Chui-Se Tham Michal Izrael Michael Webb 《ASN neuro》2014,6(4)
In inflammatory demyelinating diseases such as multiple sclerosis (MS), myelin
degradation results in loss of axonal function and eventual axonal degeneration.
Differentiation of resident oligodendrocyte precursor cells (OPCs) leading to
remyelination of denuded axons occurs regularly in early stages of MS but halts as
the pathology transitions into progressive MS. Pharmacological potentiation of
endogenous OPC maturation and remyelination is now recognized as a promising
therapeutic approach for MS. In this study, we analyzed the effects of modulating the
Rho-A/Rho-associated kinase (ROCK) signaling pathway, by the use of selective
inhibitors of ROCK, on the transformation of OPCs into mature, myelinating
oligodendrocytes. Here we demonstrate, with the use of cellular cultures from rodent
and human origin, that ROCK inhibition in OPCs results in a significant generation of
branches and cell processes in early differentiation stages, followed by accelerated
production of myelin protein as an indication of advanced maturation. Furthermore,
inhibition of ROCK enhanced myelin formation in cocultures of human OPCs and neurons
and remyelination in rat cerebellar tissue explants previously demyelinated with
lysolecithin. Our findings indicate that by direct inhibition of this signaling
molecule, the OPC differentiation program is activated resulting in morphological and
functional cell maturation, myelin formation, and regeneration. Altogether, we show
evidence of modulation of the Rho-A/ROCK signaling pathway as a viable target for the
induction of remyelination in demyelinating pathologies. 相似文献