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排序方式: 共有387条查询结果,搜索用时 15 毫秒
1.
Recognition of the neural chemoattractant Netrin-1 by integrins alpha6beta4 and alpha3beta1 regulates epithelial cell adhesion and migration 总被引:1,自引:0,他引:1
Yebra M Montgomery AM Diaferia GR Kaido T Silletti S Perez B Just ML Hildbrand S Hurford R Florkiewicz E Tessier-Lavigne M Cirulli V 《Developmental cell》2003,5(5):695-707
Netrins, axon guidance cues in the CNS, have also been detected in epithelial tissues. In this study, using the embryonic pancreas as a model system, we show that Netrin-1 is expressed in a discrete population of epithelial cells, localizes to basal membranes, and specifically associates with elements of the extracellular matrix. We demonstrate that alpha6beta4 integrin mediates pancreatic epithelial cell adhesion to Netrin-1, whereas recruitment of alpha6beta4 and alpha3beta1 regulate the migration of CK19+/PDX1+ putative pancreatic progenitors on Netrin-1. These results provide evidence for the activation of epithelial cell adhesion and migration by a neural chemoattractant, and identify Netrin-1/integrin interactions as adhesive/guidance cues for epithelial cells. 相似文献
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Francis J. Castellino Zhong Liang Patrick K. Davis Rashna D. Balsara Harsha Musunuru Deborah L. Donahue Denise L. Smith Mayra J. Sandoval-Cooper Victoria A. Ploplis Mark Walsh 《PloS one》2012,7(12)
To delineate the critical features of platelets required for formation and stability of thrombi, thromboelastography and platelet aggregation measurements were employed on whole blood of normal patients and of those with Bernard-Soulier Syndrome (BSS) and Glanzmann’s Thrombasthenia (GT). We found that separation of platelet activation, as assessed by platelet aggregation, from that needed to form viscoelastic stable whole blood thrombi, occurred. In normal human blood, ristocetin and collagen aggregated platelets, but did not induce strong viscoelastic thrombi. However, ADP, arachidonic acid, thrombin, and protease-activated-receptor-1 and -4 agonists, stimulated both processes. During this study, we identified the genetic basis of a very rare double heterozygous GP1b deficiency in a BSS patient, along with a new homozygous GP1b inactivating mutation in another BSS patient. In BSS whole blood, ADP responsiveness, as measured by thrombus strength, was diminished, while ADP-induced platelet aggregation was normal. Further, the platelets of 3 additional GT patients showed very weak whole blood platelet aggregation toward the above agonists and provided whole blood thrombi of very low viscoelastic strength. These results indicate that measurements of platelet counts and platelet aggregability do not necessarily correlate with generation of stable thrombi, a potentially significant feature in patient clinical outcomes. 相似文献
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Blanca Domenech‐Ximenos Victor Cuba Pepus Daunis‐i‐Estadella Santiago Thi‐Henestrosa Francisco Jaldo Carles Biarnes Xavier Molina Gemma Xifra Wifredo Ricart Anton Bardera Imma Boada Marco Essig Salvador Pedraza Massimo Federici Jos Manuel Fernndez‐Real Josep Puig 《Obesity (Silver Spring, Md.)》2020,28(9):1663-1670
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Livia Pilatti PhD Renato Mancini Astray Mayra Pereira Rocca Flavia Ferreira Barbosa Soraia Attie Calil Jorge Michael Butler Elisabeth de Fátima Pires Augusto 《Biotechnology progress》2020,36(6):e3046
Most rabies vaccines are based on inactivated virus, which production process demands a high level of biosafety structures. In the past decades, recombinant rabies virus glycoprotein (RVGP) produced in several expression systems has been extensively studied to be used as an alternative vaccine. The immunogenic characteristics of this protein depend on its correct conformation, which is present only after the correct post-translational modifications, typically performed by animal cells. The main challenge of using this protein as a vaccine candidate is to keep its trimeric conformation after the purification process. We describe here a new immunoaffinity chromatography method using a monoclonal antibody for RVGP Site II for purification of recombinant rabies virus glycoprotein expressed on the membrane of Drosophila melanogaster S2 cells. RVGP recovery achieved at least 93%, and characterization analysis showed that the main antigenic proprieties were preserved after purification. 相似文献
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Daniel Romero-Trejo Rosalinda Mejía-Rodríguez Edith Sierra-Mondragón Araceli Navarrete Mayra Pérez-Tapia Rosa O. González José Segovia 《Cytotherapy》2021,23(3):223-235
Background aimsMetastasis to different organs is the major cause of death in breast cancer patients. The poor clinical prognosis and lack of successful treatments for metastatic breast cancer patients demand the development of new tumor-selective therapies. Thus, it is necessary to develop treatments capable of releasing therapeutic agents to both primary tumors and metastases that avoid toxic side effects in normal tissue, and neural stem cells are an attractive vehicle for tracking tumor cells and delivering anti-cancer agents. The authorspreviously demonstrated that a soluble form of growth arrest specific 1 (GAS1) inhibits the growth of triple-negative breast tumors and glioblastoma.MethodsIn this study, the authors engineered ReNcell CX (EMD Millipore, Temecula, CA, USA) neural progenitor cells to express truncated GAS1 (tGAS1) under a tetracycline/on inducible system using lentiviral vectors.ResultsHere the authors show that treatment with ReNcell-tGAS1 in combination with tetracycline decreased primary tumor growth and inhibited the formation of metastases in tumor-bearing mice by diminishing the phosphorylation of AKT and ERK1/2 in orthotopic mammary gland tumors. Moreover, the authors observed that ReNcell-tGAS1 prolonged the survival of 4T1 tumor-bearing mice.ConclusionsThese data suggest that the delivery of tGAS1 by ReNcell cells could be an effective adjuvant for the treatment of triple-negative breast cancer. 相似文献
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Aurélie Crabbé Sheila M. Nielsen-Preiss Christine M. Woolley Jennifer Barrila Kent Buchanan James McCracken Diane O. Inglis Stephen C. Searles Mayra A. Nelman-Gonzalez C. Mark Ott James W. Wilson Duane L. Pierson Heidemarie M. Stefanyshyn-Piper Linda E. Hyman Cheryl A. Nickerson 《PloS one》2013,8(12)
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Mayra Cuéllar-Cruz Gerardo Gutiérrez-Sánchez Everardo López-Romero Estela Ruiz-Baca Julio C. Villagómez-Castro Lucio Rodríguez-Sifuentes 《Central European Journal of Biology》2013,8(4):337-345
In the past two decades, Candida species have become the second leading cause of invasive mycosis in immunocompromised patients. In order to colonize their hosts, these microorganisms express adhesins and cell wall proteins that allow them to adhere and neutralize the reactive oxygen species produced by phagocytic cells during the respiratory burst. However, the precise mechanism by which Candida cell wall proteins change their expression in response to oxidative stress has not been described. In an attempt to understand this change in response to oxidative stress, in this study, three Candida species, namely, C. albicans, C. glabrata and C. krusei, were exposed to increasing concentrations of H2O2 and induced cell wall proteins were identified by two-dimensional gel electrophoresis and peptide mass fingerprinting. Sequence analysis of differential proteins led to the identification of two heat-shock proteins in C. albicans, two enolases in C. glabrata and one enolase in C. krusei. Enolases may be involved in the protection of pathogenic cells against oxidative stress as suggested by the decrease in their expression when they were exposed to high concentrations of H2O2. To our knowledge, this is the first demonstration that expression of these proteins changes in response to oxidative stress in different Candida species. This knowledge can eventually facilitate both an early diagnosis and a more efficient treatment of this mycosis. 相似文献
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Mayra Domínguez-Pérez Arturo Simoni-Nieves Patricia Rosales Natalia Nuño-Lámbarri Mónica Rosas-Lemus Verónica Souza Roxana U. Miranda Leticia Bucio Salvador Uribe Carvajal Jens U. Marquardt Daekwan Seo Luis E. Gomez-Quiroz María Concepción Gutiérrez-Ruiz 《Journal of cellular physiology》2019,234(5):7213-7223