全文获取类型
收费全文 | 1105篇 |
免费 | 102篇 |
专业分类
1207篇 |
出版年
2023年 | 17篇 |
2022年 | 22篇 |
2021年 | 47篇 |
2020年 | 21篇 |
2019年 | 26篇 |
2018年 | 29篇 |
2017年 | 25篇 |
2016年 | 36篇 |
2015年 | 77篇 |
2014年 | 67篇 |
2013年 | 76篇 |
2012年 | 99篇 |
2011年 | 75篇 |
2010年 | 49篇 |
2009年 | 53篇 |
2008年 | 49篇 |
2007年 | 53篇 |
2006年 | 41篇 |
2005年 | 21篇 |
2004年 | 36篇 |
2003年 | 34篇 |
2002年 | 27篇 |
2001年 | 12篇 |
2000年 | 16篇 |
1999年 | 13篇 |
1998年 | 13篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1993年 | 5篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1990年 | 8篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1977年 | 3篇 |
1976年 | 7篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1969年 | 3篇 |
1968年 | 6篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1911年 | 3篇 |
排序方式: 共有1207条查询结果,搜索用时 15 毫秒
111.
112.
1. A cactus, Opuntia stricta , has invaded almost 16 000 ha of conserved, natural habitatand has become a major weed problem in Kruger National Park (KNP), South Africa.
2. The main objectives in the control of O. stricta are to reduce the density of the weed and to curb long-range dispersal of seeds by preventing young plants from reaching the size (28 cladodes) at which they start to produce fruits.
3. Herbicides have failed to provide satisfactory control of O. stricta because the weed infestations are replenished from seeds in the soil and from small plants that are overlooked during spraying.
4. A phycitid moth, Cactoblastis cactorum , was released in KNP during 1988 in an attempt to control O. stricta biologically.
5. Population counts of the biological control agent and of the weed over a 5-year period showed that, even though C. cactorum has not provided complete control of O. stricta in KNP, the moderate levels of larval damage have stunted the growth of O. stricta and have considerably extended the time that the young plants take to reach sexual maturity.
6. Comparisons of modelled (i.e. with no C. cactorum ) and actual populations of O. stricta showed that C. cactorum is making a substantial contribution to the control of O. stricta in residual infestations of the weed that have been treated with herbicides.
7. The need for long-term evaluation studies in biological weed control is demon strated by the development of an integrated management programme for effective control of O. stricta . 相似文献
2. The main objectives in the control of O. stricta are to reduce the density of the weed and to curb long-range dispersal of seeds by preventing young plants from reaching the size (28 cladodes) at which they start to produce fruits.
3. Herbicides have failed to provide satisfactory control of O. stricta because the weed infestations are replenished from seeds in the soil and from small plants that are overlooked during spraying.
4. A phycitid moth, Cactoblastis cactorum , was released in KNP during 1988 in an attempt to control O. stricta biologically.
5. Population counts of the biological control agent and of the weed over a 5-year period showed that, even though C. cactorum has not provided complete control of O. stricta in KNP, the moderate levels of larval damage have stunted the growth of O. stricta and have considerably extended the time that the young plants take to reach sexual maturity.
6. Comparisons of modelled (i.e. with no C. cactorum ) and actual populations of O. stricta showed that C. cactorum is making a substantial contribution to the control of O. stricta in residual infestations of the weed that have been treated with herbicides.
7. The need for long-term evaluation studies in biological weed control is demon strated by the development of an integrated management programme for effective control of O. stricta . 相似文献
113.
High background noise is an impediment to signal detection and perception. We report the use of multiple solutions to improve signal perception in the acoustic and visual modality by the Bornean rock frog, Staurois parvus. We discovered that vocal communication was not impaired by continuous abiotic background noise characterised by fast-flowing water. Males modified amplitude, pitch, repetition rate and duration of notes within their advertisement call. The difference in sound pressure between advertisement calls and background noise at the call dominant frequency of 5578 Hz was 8 dB, a difference sufficient for receiver detection. In addition, males used several visual signals to communicate with conspecifics with foot flagging and foot flashing being the most common and conspicuous visual displays, followed by arm waving, upright posture, crouching, and an open-mouth display. We used acoustic playback experiments to test the efficacy-based alerting signal hypothesis of multimodal communication. In support of the alerting hypothesis, we found that acoustic signals and foot flagging are functionally linked with advertisement calling preceding foot flagging. We conclude that S. parvus has solved the problem of continuous broadband low-frequency noise by both modifying its advertisement call in multiple ways and by using numerous visual signals. This is the first example of a frog using multiple acoustic and visual solutions to communicate in an environment characterised by continuous noise. 相似文献
114.
Dola Das Ehsan Fayazzadeh Xin Li Nischal Koirala Akshay Wadera Min Lang Maximilian Zernic Catherine Panick Pete Nesbitt Gordon McLennan 《Journal of cellular physiology》2020,235(9):6167-6182
Hepatocellular carcinoma (HCC) is a major health problem worldwide and in the United States as its incidence has increased substantially within the past two decades. HCC therapy remains a challenge, primarily due to underlying liver disorders such as cirrhosis that determines treatment approach and efficacy. Activated hepatic stellate cells (A-HSCs) are the key cell types involved in hepatic fibrosis/cirrhosis. A-HSCs are important constituents of HCC tumor microenvironment (TME) and support tumor growth, chemotherapy resistance, cancer cell migration, and escaping immune surveillance. This makes A-HSCs an important therapeutic target in hepatic fibrosis/cirrhosis as well as in HCC. Although many studies have reported the role of A-HSCs in cancer generation and investigated the therapeutic potential of A-HSCs reversion in cancer arrest, not much is known about inactivated or quiescent HSCs (Q-HSCs) in cancer growth or arrest. Here we report that Q-HSCs resist cancer cell growth by inducing cytotoxicity and enhancing chemotherapy sensitivity. We observed that the conditioned media from Q-HSCs (Q-HSCCM) induces cancer cell death through a caspase-independent mechanism that involves an increase in apoptosis-inducing factor expression, nuclear localization, DNA fragmentation, and cell death. We further observed that Q-HSCCM enhanced the efficiency of doxorubicin, as measured by cell viability assay. Exosomes present in the conditioned media were not involved in the mechanism, which suggests the role of other factors (proteins, metabolites, or microRNA) secreted by the cells. Identification and characterization of these factors are important in the development of effective HCC therapy. 相似文献
115.
The protease caspase-9 is activated on the apoptosome, a multiprotein signal transduction platform that assembles in response to mitochondria-dependent apoptosis initiation. Despite extensive molecular research, the assembly of the holo-apoptosome and the process of caspase-9 activation remain incompletely understood. Here, we therefore integrated quantitative data on the molecular interactions and proteolytic processes during apoptosome formation and apoptosis execution and conducted mathematical simulations to investigate the resulting biochemical signaling, quantitatively and kinetically. Interestingly, when implementing the homodimerization of procaspase-9 as a prerequisite for activation, the calculated kinetics of apoptosis execution and the efficacy of caspase-3 activation failed to replicate experimental data. In contrast, assuming a scenario in which procaspase-9 is activated allosterically upon binding to the apoptosome backbone, the mathematical simulations quantitatively and kinetically reproduced all experimental data. These data included a XIAP threshold concentration at which apoptosis execution is suppressed in HeLa cervical cancer cells, half-times of procaspase-9 processing, as well as the molecular timer function of the apoptosome. Our study therefore provides novel mechanistic insight into apoptosome-dependent apoptosis execution and suggests that caspase-9 is activated allosterically by binding to the apoptosome backbone. Our findings challenge the currently prevailing dogma that all initiator procaspases require homodimerization for activation. 相似文献
116.
Yan Y Zheng J Xiao Y Yu J Hu Y Cai M Li Y Hsam SL Zeller FJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(7):1349-1358
A novel y-type high-molecular-weight glutenin subunit possessing a slightly faster mobility than that of subunit 1Dy12 in SDS-PAGE, designated 1Dy12.1t in Aegilops tauschi, was identified by one- and two-dimensional gel and capillary electrophoresis. Its coding gene at the Glu-D
t
1 locus was amplified with allele-specific-PCR primers, and the amplified products were cloned and sequenced. The complete nucleotide sequence of 2,807 bp containing an open reading frame of 1,950 bp and 857 bp of upstream sequence was obtained. A perfectly conserved enhancer sequence and the –300 element were present at positions of 209–246 bp and 424–447 bp upstream of the ATG start codon, respectively. The deduced mature protein of 1 Dy12.1t subunit comprised 648 amino acid residues and had a Mr of 67,518 Da, which is slightly smaller than the 1Dy12 (68,695 Da) but larger than the 1Dy10 (67,495 Da) subunits of bread wheat, respectively, and corresponds well with their relative mobilities when separated by acid-PAGE. The deduced amino acid sequence indicated that the 1Dy12.1t subunit displayed a greater similarity to the 1Dy10 subunit, with only seven amino acid substitutions, suggesting that this novel gene could have positive effect on bread-making quality. A phenetic tree produced by nucleotide sequences showed that the x- and y-type subunit genes were respectively clustered together and that the Glu-D
t
1y12.1 gene of Ae. tauschii is closely related to other y-type subunit genes from the B and D genomes of hexaploid bread wheat.Communicated by H.F. Linskens 相似文献
117.
118.
Ahmed AA Mohamed Ael-H Tzakou O Petropoulou A Hassan ME El-Maghraby MA Zeller KP 《Phytochemistry》2003,62(8):1191-1194
The aerial parts of Inula verbascifolia afforded two new xanthanes and a new germacranolide derivative, together with the known compounds inusoniolide, 4-O-dihydroinusoniolide and 9beta-hydroxyparthenolide. The structures were determined by spectral methods (IR, HRMS,1H NMR, 13C NMR, DEPT, 1H-1H COSY, HMQC and HMBC). 相似文献
119.
120.
A new scheme for integrated optical fluorescence sensing is presented. The principle is based on a planar waveguide containing multiple sensing units, each consisting of a single-pad grating coupler structure. Single-pad means that all the following functions are incorporated in one single pad: laser light input, excitation of the labeled analyte molecules, efficient collection of the emitted fluorescent light into the waveguide, background suppression, and coupling the guided wave out to the detector. The results demonstrate a high efficiency of the fluorescence light excitation and collection, as well as a good suppression of the volume background. 相似文献