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951.
Zhen-Yong Keck Sven G. Enterlein Katie A. Howell Hong Vu Sergey Shulenin Kelly L. Warfield Jeffrey W. Froude Nazli Araghi Robin Douglas Julia Biggins Calli M. Lear-Rooney Ariel S. Wirchnianski Patrick Lau Yong Wang Andrew S. Herbert John M. Dye Pamela J. Glass Frederick W. Holtsberg Steven K. H. Foung M. Javad Aman 《Journal of virology》2016,90(1):279-291
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Adriana Goncalves Pateh Makalo Hassan Joof Sarah Burr Athumani Ramadhani Patrick Massae Aiweda Malisa Tara Mtuy Tamsyn Derrick Anna R. Last Meno Nabicassa Eunice Cassama Joanna Houghton Christine D. Palmer Harry Pickering Matthew J. Burton David C. W. Mabey Robin L. Bailey Martin R. Goodier Martin J. Holland Chrissy h. Roberts 《Human genetics》2016,135(8):939-951
NKG2C is an activating receptor that is preferentially expressed on natural killer (NK) cells. The gene encoding NKG2C (killer cell lectin-like receptor C2, KLRC2) is present at different copy numbers in the genomes of different individuals. Deletion at the NKG2C locus was investigated in a case–control study of 1522 individuals indigenous to East- and West-Africa and the association with the ocular Chlamydia trachomatis infection and its sequelae was explored. The frequency of homozygous KLRC2 deletion was 13.7 % in Gambians and 4.7 % in Tanzanians. A significantly higher frequency of the deletion allele was found in West-Africans from the Gambia and Guinea-Bissau (36.2 % p = 2.105 × 10?8, 26.8 % p = 0.050; respectively) in comparison to East-African Tanzanians where the frequency of the deletion is comparable to other human populations (20.9 %). We found no evidence for an association between the numbers of KLRC2 gene copies and the clinical manifestations of trachoma (follicular trachoma or conjunctival scarring). A new method for imputation of KLRC2 genotypes from single nucleotide polymorphism (SNP) data in 2621 individuals from the Gambia further confirmed these results. Our data suggest that NKG2C does not play a major role in trachomatous disease. We found that the deletion allele is present at different frequencies in different populations but the reason behind these differences is currently not understood. The new method offers the potential to use SNP arrays from genome wide association studies to study the frequency of KLRC2 deletion in other populations and its association with other diseases. 相似文献
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Laetitia Herrmann Didier Lesueur Lambert Bräu John Davison Teele Jairus Henri Robain Agnès Robin Martti Vasar Wanpen Wiriyakitnateekul Maarja Öpik 《Mycorrhiza》2016,26(8):863-877
Rubber tree (Hevea brasiliensis) is of major economic importance in Southeast Asia and for small land holders in Thailand in particular. Due to the high value of latex, plantations are expanding into unsuitable areas, such as the northeast province of Thailand where soil fertility is very low and therefore appropriate management practices are of primary importance. Arbuscular mycorrhizal fungi (AMF) contribute to plant growth through a range of mechanisms and could play a key role in a more sustainable management of the rubber plantations. We described the diversity of AMF associated with rubber tree roots in Northeast Thailand in relation to tree age and soil parameters along a chronosequence of rubber tree plantations. Cassava fields were included for comparison. Rubber tree and cassava roots harbored high diversity of AMF (111 Virtual Taxa, VT), including 20 novel VT. AMF VT richness per sample was consistently high (per site mean 16 to 21 VT per sample) along the chronosequence and was not related to soil properties. The composition of AMF communities differed between cassava and rubber tree plantations and was influenced by soil texture and nutrient content (sand, K, P, Ca). AMF community composition gradually shifted with the age of the trees. Our results suggest that the high diversity of AMF in this region is potentially significant for maintaining high functionality of AMF communities. 相似文献
956.
Fauvart M Braeken K Daniels R Vos K Ndayizeye M Noben JP Robben J Vanderleyden J Michiels J 《Biochimica et biophysica acta》2007,1774(9):1092-1098
Phylogenetic analysis of the superfamily of D-2-hydroxyacid dehydrogenases identified the previously unrecognized cluster of glyoxylate/hydroxypyruvate reductases (GHPR). Based on the genome sequence of Rhizobium etli, the nodulating endosymbiont of the common bean plant, we predicted a putative 3-phosphoglycerate dehydrogenase to exhibit GHPR activity instead. The protein was overexpressed and purified. The enzyme is homodimeric under native conditions and is indeed capable of reducing both glyoxylate and hydroxypyruvate. Other substrates are phenylpyruvate and ketobutyrate. The highest activity was observed with glyoxylate and phenylpyruvate, both having approximately the same kcat/Km ratio. This kind of substrate specificity has not been reported previously for a GHPR. The optimal pH for the reduction of phenylpyruvate to phenyllactate is pH 7. These data lend support to the idea of predicting enzymatic substrate specificity based on phylogenetic clustering. 相似文献
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The regulation by light of the composition of the photosynthetic apparatus was investigated in photomorphogenic mutants of
Arabidopsis thaliana (L.) Heynh. cv. Landsberg erecta. Leaf chlorophyll, photosynthesis, photosystem II function, and ribulose-1,5-bisphosphate carboxylase-oxygenase and photosystem
II contents were determined for plants grown under high- or low-irradiance growth regimes. Although certain mutant lines had
altered chloroplast composition compared to the wild type, all photoreceptor mutants tested were capable of light-dependent
changes in chloroplast composition and photosynthetic function, indicating that photoreceptors do not play a central role
in the regulation of acclimation at the level of the chloroplast. However, the clear acclimation defect in a det1 signal transduction mutant indicates that photoreceptor-controlled responses either share regulatory components with acclimation,
or are important in the expression of components which in turn regulate acclimation. We suggest that the COP/DET/FUS regulatory
cluster is a focus for multiple signal transduction pathways, including some of the metabolic signals which form the basis
for the acclimatory response.
Received: 22 April 1999 / Accepted: 6 June 1999 相似文献
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