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61.
62.
Max R. Brown Hannes Becher Sebastian Williams Alex D. Twyford 《American journal of botany》2023,110(1):e16100
Premise
Strong postzygotic reproductive isolating barriers are usually expected to limit the extent of natural hybridization between species with contrasting ploidy. However, genomic sequencing has revealed previously overlooked examples of natural cross-ploidy hybridization in some flowering plant genera, suggesting that the phenomenon may be more common than once thought. We investigated potential cross-ploidy hybridization in British eyebrights (Euphrasia, Orobanchaceae), a group from which 13 putative cross-ploidy hybrid combinations have been reported based on morphology.Methods
We analyzed a contact zone between diploid Euphrasia rostkoviana and tetraploid E. arctica in Wales. We sequenced part of the internal transcribed spacer (ITS) of nuclear ribosomal DNA and used genotyping by sequencing (GBS) to look for evidence of cross-ploidy hybridization and introgression.Results
Common variant sites in the ITS region were fixed between diploids and tetraploids, indicating a strong barrier to hybridization. Clustering analyses of 356 single-nucleotide polymorphisms (SNPs) generated using GBS clearly separated samples by ploidy and revealed strong genetic structure (FST = 0.44). However, the FST distribution across all SNPs was bimodal, indicating potential differential selection on loci between diploids and tetraploids. Demographic inference suggested potential gene flow, limited to around one or fewer migrants per generation.Conclusions
Our results suggest that recent cross-ploidy hybridization is rare or absent in a site of secondary contact in Euphrasia. While a strong ploidy barrier prevents hybridization over ecological timescales, such hybrids may form in stable populations over evolutionary timescales, potentially allowing cross-ploidy introgression to take place. 相似文献63.
64.
Short-term (24–48 h) colonization dynamics of periphytic diatoms on artificial (styrofoam) substrata were examined using fast-flushing, continuous-flow troughs located on the North Thompson River, British Columbia. Two parallel troughs, one exposed to natural light and the other completely darkened, showed significant differences in periphyton biomass, chlorophyll a, and algal taxonomic composition with 24 h. Experiments which commenced at the onset of natural darkness demonstrated that rates of algal immigration during the night were the same in both troughs. Within 2–3 h of sunrise, however, certain diatom species (most notably Hannaea arcus (Ehr.) Pair, and Diatoma tenue Ag.) selectively emigrated from the artificially darkened trough but remained in the trough exposed to natural light. More closely adhering species such as Achnanthes minutissima Kütz, also showed significant emigration from the darkened trough after light deprivation for two photoperiods. Data from adhesion, emigration, and sinking rate experiments indicate that differential egress of cells from the darkened versus the lighted environments is the result of cellular regulation of buoyancy or form resistance. 相似文献
65.
Tulin Sahinoglu Cliff R. Stevens Bhupendra Bhatt David R. Blake 《Methods (San Diego, Calif.)》1996,9(3):628-634
The production of reactive oxidants has been implicated in the pathology of a number of inflammatory conditions, including inflamed arthritic joints. Many assays for the detection of these oxidants in diseased states have been described, but there are a number of potential pitfalls in both experimental design and the interpretation of results obtained with these techniques. Here, we describe a number of commonly used assays to detect the production of reactive oxidants and critically discuss their usefulness and limitations. We focus on the role of xanthine oxidase in reactive oxidant production in inflammatory disease. 相似文献
66.
Swamp gorillas in northern Congo 总被引:3,自引:0,他引:3
67.
Spatial and Temporal variations of mangrove fish assemblages in Martinique (French West Indies) 总被引:1,自引:1,他引:0
A study of the mangrove fish fauna in a bay of Martinique Island (French West Indies) was carried out at different seasons during two consecutive years. fishes were sampled with specific hoop-nets in the coastal areas at 8 stations.A total of 87 species was collected in the bay. Most individuals were represented by small-size specimens and juveniles. The overall species richness varied according to the stations and the sampling periods. The biomass and number of individuals were variable according to the location but remained stable in time. A factor correspondence analysis and a hierarchical clustering with median links were used to follow the evolution of the stations in space and time. Two types of stations were differentiated: the stations characterized by the mangrove and those under the influence of seagrass beds. A seasonal cycle, opposing the dry periods to the others, was observed.Thus, it seems that the use of the mangrove habitat by the fishes is optimized through a complete reorganization of communities in terms of species composition whereas the overall number and biomass remain stable. This model remains valid even for the most constraining biota of the mangrove ecosystem inhabited by a small number of well adapted species. 相似文献
68.
69.
Coloboma Hyperactive Mutant Mice Exhibit Regional and Transmitter-Specific Deficits in Neurotransmission 总被引:3,自引:1,他引:2
Jacob Raber Prashant P. Mehta Max Kreifeldt Loren H. Parsons Friebert Weiss Floyd E. Bloom Michael C. Wilson 《Journal of neurochemistry》1997,68(1):176-186
Abstract: The mouse mutant coloboma ( Cm /+), which exhibits profound spontaneous hyperactivity and bears a deletion mutation on chromosome 2, including the gene encoding synaptosomal protein SNAP-25, has been proposed to model aspects of attention-deficit hyperactivity disorder. Increasing evidence suggests a crucial role for SNAP-25 in the release of both classical neurotransmitters and neuropeptides. In the present study, we compared the release of specific neurotransmitters in vitro from synaptosomes and slices of selected brain regions from Cm /+ mice with that of +/+ mice. The release of dopamine (DA) and serotonin (5-HT) from striatum, and of arginine vasopressin and corticotropin-releasing factor from hypothalamus and amygdala is calcium-dependent. Glutamate release from and content in cortical synaptosomes of Cm /+ mice are greatly reduced, which might contribute to the learning deficits in these mutants. In dorsal striatum of Cm /+ mutants, but not ventral striatum, KCI-induced release of DA is completely blocked and that of 5-HT is significantly attenuated, suggesting that striatal DA and 5-HT deficiencies may be involved in hyperactivity. Further, although acetylcholine failed to induce hypothalamic corticotropin-releasing factor release from Cm /+ slices, restraint stress increased plasma corticosterone levels in Cm /+ mice to a significantly higher level than in +/+ mice, suggesting an important role for arginine vasopressin in hypothalamic-pituitary-adrenal axis activation. These results suggest that reduced SNAP-25 expression may contribute to a region-specific and neurotransmitter-specific deficiency in neurotransmitter release. 相似文献
70.
Sato Yoji; Weil Max Harry; Tang Wanchun; Sun Shijie; Xie Jianlin; Bisera Joe; Hosaka Hidehiro 《Journal of applied physiology》1997,82(2):558-562
Sato, Yoji, Max Harry Weil, Wanchun Tang, Shijie Sun,Jianlin Xie, Joe Bisera, and Hidehiro Hosaka. EsophagealPCO2 as a monitor of perfusionfailure during hemorrhagic shock. J. Appl.Physiol. 82(2): 558-562, 1997.Measurement ofgastric wall PCO2(PgCO2) bytonometric method has emerged as an attractive option for estimatingvisceral perfusion during circulatory shock. However, gastric acidsecretion obfuscates the tonometric measurement. We, therefore,investigated the option of measuringPCO2 in the esophagus to minimizethese restraints. Hemorrhagic shock was induced in five Sprague-Dawleyrats, and five rats served as sham controls.PgCO2 wasmeasured with an ion-sensitive field effect transistor that wassurgically implanted into the gastric wall. Esophageal luminalPCO2(PeCO2) wasmeasured by a second ion-sensitive field effect transistor sensor.During hemorrhagic shock, mean aortic pressure declined from 150 to 50 mmHg. Gastric blood flow decreased from 58 to 12 ml · min1 · 100 g1 (21% of preshock) andesophageal blood flow from 44 to 7 ml · min1 · 100 g1 (16% of preshock).PgCO2simultaneously increased from 47 to 116 Torr andPeCO2 from 47 to 127 Torr. The increases inPgCO2 werehighly correlated with increases inPeCO2(r = 0.90). Esophageal tonometry may,therefore, serve as a practical alternative to gastric tonometry. 相似文献