全文获取类型
收费全文 | 2565篇 |
免费 | 177篇 |
专业分类
2742篇 |
出版年
2023年 | 9篇 |
2022年 | 19篇 |
2021年 | 33篇 |
2020年 | 27篇 |
2019年 | 25篇 |
2018年 | 50篇 |
2017年 | 47篇 |
2016年 | 76篇 |
2015年 | 104篇 |
2014年 | 122篇 |
2013年 | 188篇 |
2012年 | 194篇 |
2011年 | 176篇 |
2010年 | 137篇 |
2009年 | 126篇 |
2008年 | 172篇 |
2007年 | 159篇 |
2006年 | 160篇 |
2005年 | 125篇 |
2004年 | 152篇 |
2003年 | 113篇 |
2002年 | 114篇 |
2001年 | 18篇 |
2000年 | 12篇 |
1999年 | 30篇 |
1998年 | 28篇 |
1997年 | 21篇 |
1996年 | 19篇 |
1995年 | 30篇 |
1994年 | 18篇 |
1993年 | 23篇 |
1992年 | 20篇 |
1991年 | 16篇 |
1990年 | 24篇 |
1989年 | 16篇 |
1988年 | 10篇 |
1987年 | 5篇 |
1986年 | 6篇 |
1985年 | 6篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 11篇 |
1981年 | 16篇 |
1980年 | 8篇 |
1979年 | 5篇 |
1978年 | 10篇 |
1977年 | 7篇 |
1976年 | 8篇 |
1974年 | 12篇 |
1973年 | 4篇 |
排序方式: 共有2742条查询结果,搜索用时 15 毫秒
101.
Abstract Actually, in modern process simulators, more than 75% of the code implemented is dedicated to physical properties estimation, calculation and predictions. Data banks storing pure component parameters and binary interaction parameters for phase equilibrium calculations are extensively used and continuously implemented in actual process simulators. This gives an idea of the important role physical properties availability plays in process simulation. In this paper we propose a new way for coupling molecular and process simulation. The basic machinery is to resort to molecular/quantum mechanics and molecular dynamics simulation techniques for generating the parameters of some equations of state that will subsequently be used for the prediction of phase equilibria and PVT behavior of small and polymeric molecules as well. This information, in turn, will be used as input in the process simulator, thus creating a final and well-defined bridge between molecular and process simulations in chemical engineering. 相似文献
102.
Maurizio Cutolo 《Arthritis research & therapy》2009,11(5):126-3
Neither hormone receptor genes nor plasma androgens seem significantly altered in female subjects before they became affected
by rheumatoid arthritis (RA) and, therefore, do not seem to play a role as risk factors for its development. However, serum
testosterone levels are inversely correlated with RA activity and dehydro-epiandrosterone sulfate (DHEAS) plasma levels are
inversely correlated with both disease duration and clinical severity in patients already affected by active RA. In particular,
gonadal and adrenal androgens (that is, testosterone and DHEAS) are significantly decreased in inflamed synovial tissue/fluids
during active disease as a consequence of the inflammatory reaction, which supports a pro-inflammatory milieu in RA joints.
Recently, male gender has been found to be a major predictor of remission in early RA. 相似文献
103.
Marco Redaelli María Jimena Ricatti Marialaura Simonetto Mirko Claus Maurizio Ballabio Antonio Caretta Carla Mucignat-Caretta 《PloS one》2015,10(3)
Poor micturition control may cause profound distress, because proper voiding is mandatory for an active social life. Micturition results from the subtle interplay of central and peripheral components. It involves the coordination of autonomic and neuromuscular activity at the brainstem level, under the executive control of the prefrontal cortex. We tested the hypothesis that administration of molecules acting as reuptake inhibitors of serotonin, noradrenaline or both may exert a strong effect on the control of urine release, in a mouse model of overactive bladder. Mice were injected with cyclophosphamide (40 mg/kg), to increase micturition acts. Mice were then given one of four molecules: the serotonin reuptake inhibitor imipramine, its metabolite desipramine that acts on noradrenaline reuptake, the serotonin and noradrenaline reuptake inhibitor duloxetine or its active metabolite 4-hydroxy-duloxetine. Cyclophosphamide increased urine release without inducing overt toxicity or inflammation, except for increase in urothelium thickness. All the antidepressants were able to decrease the cyclophosphamide effects, as apparent from longer latency to the first micturition act, decreased number of urine spots and volume of released urine. These results suggest that serotonin and noradrenaline reuptake inhibitors exert a strong and effective modulatory effect on the control of urine release and prompt to additional studies on their central effects on brain areas involved in the social and behavioral control of micturition. 相似文献
104.
Climate determinants of breeding and wintering ranges of lesser kestrels in Italy and predicted impacts of climate change 下载免费PDF全文
Michelangelo Morganti Damiano Preatoni Maurizio Sarà 《Journal of avian biology》2017,48(12):1595-1607
Climate warming would theoretically create conditions for the breeding range expansion of pseudo‐steppe Mediterranean and long‐distance migrant species and provide the possibility for these to overwinter in the same breeding areas. However, contemporary changes in rainfall regimes might have negative effects on the climate suitability and in turn, shrink species potential range. The lesser kestrel Falco naumanni is highly sensitive to rainfall oscillations and has recently extended its Italian breeding range towards northern latitudes and increasing its wintering records. We modelled the effects of temperature and rainfall on current and future climate suitability for lesser kestrels in both the breeding and wintering periods by using MaxEnt. Models were based on the distribution of 298 colonies and 40 wintering records. Future climate suitability was assessed under eight different scenarios. Spring rainfall amount resulted as the main determinant of breeding climate suitability, so its predicted reduction will determine a shrinkage in suitable areas (–42.10% in 2050; –32.07% in 2070). Specifically, the 66.05% of Italian colonies will be outside the climatically suitable area by 2050. However wide areas, suitable under current climate conditions, are still not occupied by lesser kestrel and allow the potential expansion of its Italian breeding range in the short term. Temperature seasonality mainly determined the species’ winter climate suitability, which is overall predicted to boost in the next decades (+145.03% in 2050; and +123.91% in 2070). All but one future scenarios predicted a northward shift of about 40 km for both breeding and wintering climate suitability. Despite its recent expansion, we have found that climate change will pose conservation concerns for the Italian breeding population of lesser kestrels. Indeed, changes in non‐climate factors will also outline the future suitability of the Italian range for lesser kestrels in both seasons with effects that might both strengthen or mitigate climate effects. 相似文献
105.
Andrea Masotti Chiara Laurenzi Sara Boenzi Anna Pastore Anna Taranta Francesco Bellomo Maurizio Muraca Carlo Dionisi-Vici Pierfrancesco Bertucci Luca Dello Strologo Francesco Emma 《Amino acids》2014,46(2):415-427
Cystinuria is an autosomal recessive disease that causes l-cystine precipitation in urine and nephrolithiasis. Disease severity is highly variable; it is known, however, that cystinuria has a more severe course in males. The aim of this study was to compare l-cystine metastability in first-morning urine collected from 24 normal female and 24 normal male subjects. Samples were buffered at pH 5 and loaded with l-cystine (0.4 and 4 mM final concentration) to calculate the amount remaining in solution after overnight incubation at 4 °C; results were expressed as Z scores reflecting the l-cystine solubility in each sample. In addition, metabolomic analyses were performed to identify candidate compounds that influence l-cystine solubility. l-cystine solubility Z score was +0.44 ± 1.1 and ?0.44 ± 0.70 in female and male samples, respectively (p < 0.001). Further analyses showed that the l-cystine solubility was independent from urine concentration but was significantly associated with low urinary excretion of inosine (p = 0.010), vanillylmandelic acid (VMA) (p = 0.015), adenosine (p = 0.029), and guanosine (p = 0.032). In vitro l-cystine precipitation assays confirmed that these molecules induce higher rates of l-cystine precipitation in comparison with their corresponding dideoxy molecules, used as controls. In silico computational and modeling analyses confirmed higher binding energy of these compounds. These data indicate that urinary excretion of nucleosides and VMA may represent important factors that modulate l-cystine solubility and may represent new targets for therapy in cystinuria. 相似文献
106.
107.
Christina Kennedy Natalie Doetsch Dietmar Meletzus Eduardo Patriarca Mohamad Amar Maurizio Iaccarino 《Plant and Soil》1994,161(1):43-57
A plentiful supply of fixed nitrogen as ammonium (or other compounds such as nitrate or amino acids) inhibits nitrogen fixation in free-living bacteria by preventing nitrogenase synthesis and/or activity. Ammonium and nitrate have variable effects on the ability ofRhizobiaceae (Rhizobium, Bradyrhizobium andAzorhizobium) species to nodulate legume hosts and on nitrogen fixation capacity in bacteroid cells contained in nodules or in plant-free bacterial cultures. In addition to effects on nitrogen fixation, excess ammonium can inhibit activity or expression of other pathways for utilization of nitrogenous compounds such as nitrate (through nitrate and nitrite reductase), or glutamine synthetase (GS) for assimilation of ammonium. This paper describes the roles of two key genesglnB andglnD, whose gene products sense levels of fixed nitrogen and initiate a cascade of reactions in response to nitrogen status. While work onEscherichia coli and other enteric bacteria provides the model system,glnB and, to a lesser extent,glnD have been studied in several nitrogen fixing bacteria. Such reports will be reviewed here. Recent results on the identity and function of theglnB andglnD gene products inAzotobacter vinelandii (a free-living soil diazotroph) and inRhizobium leguminosarum biovarviciae, hereinafter designatedR.l. viciae will be presented. New data suggests thatAzotobacter vinelandii probably contains aglnB-like gene and this organism may have twoglnD-like genes (one of which was recently identified and namednfrX). In addition, evidence for uridylylation of theglnB gene product (the PII protein) ofR. l. viciae in response to fixed nitrogen deficiency is presented. Also, aglnB mutant ofR. l. viciae has been isolated; its characteristics with respect to expression of nitrogen regulated genes is described. 相似文献
108.
Nadia Mastroianni Maurizio De Fusco Massimo Zollo Giulia Arrigo Orsetta Zuffardi Alberto Bettinelli Andrea Ballabio Giorgio Casari 《Genomics》1996,35(3):486
Electrolyte homeostasis is maintained by several ion transport systems. Na–(K)–Cl cotransporters promote the electrically silent movement of chloride across the membrane in absorptive and secretory epithelia. Two kidney-specific Na–(K)–Cl cotransporter isoforms are known, so far, according to their sensitivity to specific inhibitors. We have cloned the human cDNA coding for the renal Na–Cl cotransporter selectively inhibited by the thiazide class of diuretic agents. The predicted protein sequence of 1021 amino acids (112 kDa) shows a structure common to the other members of the Na–(K)–Cl cotransporter family: a central region harboring 12 transmembrane domains and the 2 intracellular hydrophilic amino and carboxyl termini. The ex- pression pattern of the human Na–Cl thiazide-sensitive cotransporter (hTSC, HGMW-approved symbol SLC12A3) confirms the kidney specificity. hTSC has been mapped to human chromosome 16q13 by fluorescencein situhybridization. The cloning and characterization of hTSC now render it possible to study the involvement of this cotransport system in the pathogenesis of tubulopathies such as Gitelman syndrome. 相似文献
109.
110.