Flavonoids variation in seven tetraploid populations of Dactylis glomerata

133 individual plants representing seven tetraploid populations of Dactytis glomerata were examined for their flavonoid pattern by using HPLC. The chemical results expressed quantitatively as peak heights were submitted to multivariate analysis. This data treatment suggested an obvious discrimination between Eurasian and Mediterranean types, lending support to the taxonomic status of the species. This segregation is based on the distribution of C-glycosylluteolins and methylated derivatives. Moreover among the Mediterranean collections, a Coraica population was conspicuously separated from the other three in regard to the distributionof di-C-glycosylflavones. This chemical approach was compared with an electrophoretic assay concerning seven enzyme systems; their respective conclusions were in good agreement.     

Polysome-dependent synthesis of embryonic proteins of Artemia salina during cell differentiation and analysis of heme-containing protein.

The polysomes isolated from the different developmental stages of Artemia salina have been translated in a homologous S-30 extract or pH 5 enzyme fraction as well as in a heterologous (wheat germ) pH 5 enzyme fraction. The specific template activity of the polysomes increases linearly up to a stage which is equivalent to about two-thirds of the whole preemergence period and thereafter remains rather constant until the nauplius stage. The specific template activity of the polysomes appears to be dependent on a source of in vitro systems, but not to be dependent on the size of the polysomes used. Moreover, the efficiency of polypeptide chain release from the polysomes in vitro also seems dependent on the developmental stages of the embryos, but in this case it is not influenced by the in vitro system employed. Analysis of the proteins synthesized in vitro by the polyacrylamide gel electrophoresis has revealed that the species and their relative amounts of the proteins are rather specific for each developmental stage analyzed.Heme-containing proteins from the nauplii have been partially purified and analyzed. This heme-protein complex contains two distinct polypeptide chains, MW > 100,000 and MW = 50,000–60,000 daltons, respectively, as the major species as judged by SDS-polyacrylamide gel electrophoresis. During preemergence development, no heme-protein complexes have been detected, but after hatching of the embryos, they have been detected.