全文获取类型
收费全文 | 2221篇 |
免费 | 181篇 |
国内免费 | 3篇 |
专业分类
2405篇 |
出版年
2021年 | 26篇 |
2019年 | 22篇 |
2017年 | 25篇 |
2016年 | 33篇 |
2015年 | 54篇 |
2014年 | 64篇 |
2013年 | 101篇 |
2012年 | 113篇 |
2011年 | 125篇 |
2010年 | 70篇 |
2009年 | 62篇 |
2008年 | 88篇 |
2007年 | 106篇 |
2006年 | 79篇 |
2005年 | 88篇 |
2004年 | 103篇 |
2003年 | 89篇 |
2002年 | 95篇 |
2001年 | 24篇 |
2000年 | 22篇 |
1999年 | 39篇 |
1998年 | 39篇 |
1997年 | 26篇 |
1996年 | 20篇 |
1995年 | 19篇 |
1994年 | 20篇 |
1993年 | 24篇 |
1992年 | 29篇 |
1991年 | 26篇 |
1990年 | 20篇 |
1989年 | 23篇 |
1988年 | 23篇 |
1987年 | 16篇 |
1985年 | 22篇 |
1984年 | 26篇 |
1982年 | 35篇 |
1981年 | 27篇 |
1980年 | 31篇 |
1979年 | 16篇 |
1978年 | 26篇 |
1977年 | 19篇 |
1976年 | 26篇 |
1975年 | 15篇 |
1974年 | 24篇 |
1973年 | 22篇 |
1972年 | 18篇 |
1963年 | 14篇 |
1961年 | 17篇 |
1959年 | 15篇 |
1957年 | 16篇 |
排序方式: 共有2405条查询结果,搜索用时 0 毫秒
51.
Maurice E. Tucker Michael J. Benton 《Palaeogeography, Palaeoclimatology, Palaeoecology》1982,40(4):361-379
A consideration of all the available data on Triassic vertebrate faunas, and their stratigraphic location reveals a relatively sudden extinction event among the last of the mammal-like reptiles and the herbivorous rhynchosaurs in the Norian of the Upper Triassic. This event was apparently quickly followed by the radiation of the dinosaurs, also in the Norian. This conclusion suggests that competition was not the main factor in the initial success of the dinosaurs, but opportunistic radiation following the extinction of major reptile groups. A global review of Triassic sedimentary facies shows that there were climatic and floral changes towards the end of the Triassic. It is envisaged that increasing aridity in the later Triassic, resulting from plate motions and particularly affecting Gondwanaland and southwestern Laurasia, brought about floral changes and then the reptile extinctions. With the rapid evolution of new floras of conifers and bennettitaleans, the dinosaurs came to dominate all terrestrial faunas within the space of only a few million years. 相似文献
52.
L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) was found to inhibit several aspects of arachidonic acid (20:4) metabolism in human platelets; the primary effect being inhibition of thromboxane synthetase. Thromboxane B2 (TxB2) formation from exogenous 20:4 or PGH2, or from endogenous 20:4, was inhibited by TPCK at concentrations between 0.1 and 0.5 mM. Formation of malondialdehyde (MDA) and 12-L-hydroxy-5,8,10-heptadecatrienoic acid (HHT), products which also arise from PGH2, was inhibited to a similar extent. Inhibition of formation from 20:4 of 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE), the product of the lipoxygenase pathway, was observed; although the extent of this inhibition was less than that of TxB2 formation. A small inhibitory effect of TPCK on the release of 20:4 from platelet phospholipids was also observed. This evidence indicated that while a number of reactions are inhibited by TPCK, the primary effect appears to be inhibition of thromboxane synthetase. 相似文献
53.
Paul A. Wiersma John E. Hachey William L. Crosby Maurice M. Moloney 《Molecular & general genetics : MGG》1990,224(1):155-159
Summary The expression of an acetolactate synthase (ALS) gene isolated from the cruciferous plant Brassica napus was investigated in Salmonella typhimurium. Using an expression plasmid containing the highly active trc (trp-lac) promoter, several plant ALS constructs were made containing successive in-frame truncations from the 5 end of the coding region. Functional complementation by these plant ALS constructs of a S. typhimurium mutant devoid of ALS enzymic activity was assayed on minimal medium. Truncations which eliminated a large portion of the transit peptide coding sequence proved to act as efficient ALS genes in the bacterial host. Truncations close to the putative processing site of the plant protein were inactive in the complementation test. A full length copy of the gene, including the entire transit peptide coding region, was also inactive. The efficiency of the complementation, estimated by comparison to the growth rate of wild-type S. typhimurium, was found to correlate with levels of ALS activity in the transformed bacteria. Specific mutations, known to produce herbicide resistance in plants, were introduced into the truncated ALS coding sequence by site-directed mutagenesis. When expressed in bacteria these constructs conferred a herbicide resistance phenotype on the host. The potential of this system for mutagenesis and enzymological studies of plant proteins is discussed. 相似文献
54.
55.
56.
In 2003, the invasive fruit fly Bactrocera invadens Drew, Tsuruta & White (Diptera: Tephritidae) (Drew et al. 2005), of possible Sri Lankan origin, has been detected in the East and about 1 yr later in West Africa. In regular surveys in Benin and Cameroon covering 4 yr, samples from 117 plant species across 43 families have been obtained. Incubation of field-collected fruits demonstrate that in West and Central Africa (WCA) B. invadens is highly polyphagous, infesting wild and cultivated fruits of at least 46 species from 23 plant families with guava (Psidium spp.), mango (Mangifera spp.), and citrus (spp.), and the wild hosts tropical almond (Terminalia catappa L.), African wild mango (Irvingia gabonensis (Aubry-Lecomte) Baill.), and sheanut (Vitellaria paradoxa C.F.Gaertn.) showing the highest infestation index. B. invadens occurs in 22 countries of WCA with new records for Angola, Central African Republic, the Congo, DR Congo, Equatorial Guinea, Gabon, Gambia, Guinea Bissau, Mali, Mauritania, Niger, and Sierra Leone. Overall, the pest has spread across a North-South distance of ?5,000 km representing a contiguous area of >8.3 million km(2) within WCA. B. invadens has adapted to a wide range of ecological and climatic conditions extending from low land rainforest to dry savanna. Because of its highly destructive and invasive potential, B. invadens poses a serious threat to horticulture in Africa if left uncontrolled. Moreover, the presence of this quarantine pest causes considerable restrictions on international trade of affected crops. 相似文献
57.
Maurice Campbell 《BMJ (Clinical research ed.)》1958,2(5106):1175-1184
58.
59.
Maurice S Coroller L Debaets S Vasseur V Le Floch G Barbier G 《Journal of applied microbiology》2011,111(6):1436-1446
Aims: To predict the risk factors for building infestation by Serpula lacrymans, which is one of the most destructive fungi causing timber decay in buildings. Methods and Results: The growth rate was assessed on malt extract agar media at temperatures between 1·5 and 45°C, at water activity (aw) over the range of 0·800–0·993 and at pH ranges from 1·5 to 11·0. The radial growth rate (μ) and the lag phase (λ) were estimated from the radial growth kinetics via the plots radius vs time. These parameters were then modelled as a function of the environmental factors tested. Models derived from the cardinal model (CM) were used to fit the experimental data and allowed an estimation of the optimal and limit values for fungal growth. Optimal growth rate occurred at 20°C, at high aw level (0·993) and at a pH range between 4·0 and 6·0. The strain effect on the temperature parameters was further evaluated using 14 strains of S. lacrymans. The robustness of the temperature model was validated on data sets measured in two different wood‐based media (Quercus robur L. and Picea abies). Conclusions: The two‐step procedure of exponential model with latency followed by the CM with inflection gives reliable predictions for the growth conditions of a filamentous fungus in our study. The procedure was validated for the study of abiotic factors on the growth rate of S. lacrymans. Significance and Impact of the Study: This work describes the usefulness of evaluating the effect of physico‐chemical factors on fungal growth in predictive building mycology. Consequently, the developed mathematical models for predicting fungal growth on a macroscopic scale can be used as a tool for risk assessment of timber decay in buildings. 相似文献
60.
Sensorimotor restriction by a 14-day period of hindlimb unloading (HU) in the adult rat induces a reorganization of topographic maps and receptive fields. However, the underlying mechanisms are still unclear. Interest was turned towards a possible implication of intracellular MAPK signaling pathway since Extracellular-signal-Regulated Kinase 1/2 (ERK1/2) is known to play a significant role in the control of synaptic plasticity. In order to better understand the mechanisms underlying cortical plasticity in adult rats submitted to a sensorimotor restriction, we analyzed the time-course of ERK1/2 activation by immunoblot and of cortical reorganization by electrophysiological recordings, on rats submitted to hindlimb unloading over four weeks. Immunohistochemistry analysis provided evidence that ERK1/2 phosphorylation was increased in layer III neurons of the somatosensory cortex. This increase was transient, and parallel to the changes in hindpaw cortical map area (layer IV). By contrast, receptive fields were progressively enlarged from 7 to 28 days of hindlimb unloading. To determine whether ERK1/2 was involved in cortical remapping, we administered a specific ERK1/2 inhibitor (PD-98059) through osmotic mini-pump in rats hindlimb unloaded for 14 days. Results demonstrate that focal inhibition of ERK1/2 pathway prevents cortical reorganization, but had no effect on receptive fields. These results suggest that ERK1/2 plays a role in the induction of cortical plasticity during hindlimb unloading. 相似文献