Genetic Heterozygosity in Unreplicated Bacteriophage λ Recombinants

Bacteriophage crosses using density-labeled parents have been carried out under conditions restricting DNA synthesis. The parental material and genetic contributions to progeny manifesting recombination within a genetic interval sufficiently short to exhibit high negative interference have been examined. The unreplicated products of recombination isolated as phage particles appear to contain long continuous heteroduplex regions which are heterozygous for the closely linked markers. Recombination between closely linked markers seems to be the consequence of the removal of base-pair mismatches that are present within the heteroduplex regions. This localized reduction of heterozygosity within the heteroduplex regions that join the parental components of recombinant DNA molecules can account for high negative interference.     

The disappearance of four leaf leachates in a hard and soft water stream in South Western Ontario,Canada

The disappearance of ‘dissolved’ organic carbon (D. O. C.) leached from maple, aspen, cedar and pine leaves was followed in hard and soft stream water and hard and soft stream water plus sediment. Differences were found in the rates and extent of the disappearance of D. O. C. between the leaves and the water types. These are discussed in relation to the productivity of two extreme stream types.     

Ants impact the composition of the aquatic macroinvertebrate communities of a myrmecophytic tank bromeliad

In an inundated Mexican forest, 89 out of 92 myrmecophytic tank bromeliads (Aechmea bracteata) housed an associated ant colony: 13 sheltered Azteca serica, 43 Dolichoderus bispinosus, and 33 Neoponera villosa. Ant presence has a positive impact on the diversity of the aquatic macroinvertebrate communities (n = 30 bromeliads studied). A Principal Component Analysis (PCA) showed that the presence and the species of ant are not correlated to bromeliad size, quantity of water, number of wells, filtered organic matter or incident radiation. The PCA and a generalized linear model showed that the presence of Azteca serica differed from the presence of the other two ant species or no ants in its effects on the aquatic invertebrate community (more predators). Therefore, both ant presence and species of ant affect the composition of the aquatic macroinvertebrate communities in the tanks of A. bracteata, likely due to ant deposition of feces and other waste in these tanks.     

Essai chimiosystématique sur la tribu des Lotées

A survey of the leaves and flowers of 62 representatives of the tribe Loteae (Leguminosae) showed the presence of several classes of flavonoids: flavonol 7-methyl ethers (rhamnocitrin, rhamnetin), 8-O-substituted flavonols (gossypetin, limocitrin, sexangularetin, corniculatusin), 3′,4′,5′-tri-O-substituted flavonols (myricetin, mearnsetin, syringetin, laricitrin), proanthocyanidins and flavone-C-glycosides. The trisubstitution of the B-ring and the 8-O-substitution of the A-ring allow the definition of a major group including the genera Dorycnium, Bonjeania, Lotus and Tetragonolobus. The presence of proanthocyanidins and 7-O-methylation determine a second group consisting of the genus Anthyllis. Finally, Securigera, on the basis of its flavonoid chemistry, appears to be rather remote from other members of the tribe.     

On the Mechanism of Integration of Transforming Deoxyribonucleate

The characteristics of the intermediates in the reaction, between DNA and pneumococcus, that results in genetic transformation are described in so far as they have been characterized. Transformation with DNA isolated from bacteria carrying in addition to genetic markers ³²P as a radioactive label and ²H and ¹⁵N as density labels has permitted the characterization of the product of recombination between the newly introduced DNA and the DNA of a recipient bacterium. The evidence for a single strand displacement mechanism producing a hybrid structure in the DNA of the recipient bacteria is presented. Progeny of single transformants have been examined. The results of these segregation studies permit the further characterization of this hybrid product of transformation as a genetically heterozygous structure.     

Advanced psychometric testing on a clinical screening tool to evaluate insomnia: sleep condition indicator in patients with advanced cancer

Sleep and Biological Rhythms - To examine the psychometric properties of the Sleep Condition Indicator (SCI) using different psychometric approaches [including classical test theory, Rasch models,...     

Compositional turnover and variation in Eemian pollen sequences in Europe

Vegetation History and Archaeobotany - The Eemian interglacial represents a natural experiment on how past vegetation with negligible human impact responded to amplified temperature changes...     

Neural Mechanisms Underlying Breathing Complexity

Breathing is maintained and controlled by a network of automatic neurons in the brainstem that generate respiratory rhythm and receive regulatory inputs. Breathing complexity therefore arises from respiratory central pattern generators modulated by peripheral and supra-spinal inputs. Very little is known on the brainstem neural substrates underlying breathing complexity in humans. We used both experimental and theoretical approaches to decipher these mechanisms in healthy humans and patients with chronic obstructive pulmonary disease (COPD). COPD is the most frequent chronic lung disease in the general population mainly due to tobacco smoke. In patients, airflow obstruction associated with hyperinflation and respiratory muscles weakness are key factors contributing to load-capacity imbalance and hence increased respiratory drive. Unexpectedly, we found that the patients breathed with a higher level of complexity during inspiration and expiration than controls. Using functional magnetic resonance imaging (fMRI), we scanned the brain of the participants to analyze the activity of two small regions involved in respiratory rhythmogenesis, the rostral ventro-lateral (VL) medulla (pre-Bötzinger complex) and the caudal VL pons (parafacial group). fMRI revealed in controls higher activity of the VL medulla suggesting active inspiration, while in patients higher activity of the VL pons suggesting active expiration. COPD patients reactivate the parafacial to sustain ventilation. These findings may be involved in the onset of respiratory failure when the neural network becomes overwhelmed by respiratory overload We show that central neural activity correlates with airflow complexity in healthy subjects and COPD patients, at rest and during inspiratory loading. We finally used a theoretical approach of respiratory rhythmogenesis that reproduces the kernel activity of neurons involved in the automatic breathing. The model reveals how a chaotic activity in neurons can contribute to chaos in airflow and reproduces key experimental fMRI findings.