Essai chimiosystématique sur la tribu des Lotées

A survey of the leaves and flowers of 62 representatives of the tribe Loteae (Leguminosae) showed the presence of several classes of flavonoids: flavonol 7-methyl ethers (rhamnocitrin, rhamnetin), 8-O-substituted flavonols (gossypetin, limocitrin, sexangularetin, corniculatusin), 3′,4′,5′-tri-O-substituted flavonols (myricetin, mearnsetin, syringetin, laricitrin), proanthocyanidins and flavone-C-glycosides. The trisubstitution of the B-ring and the 8-O-substitution of the A-ring allow the definition of a major group including the genera Dorycnium, Bonjeania, Lotus and Tetragonolobus. The presence of proanthocyanidins and 7-O-methylation determine a second group consisting of the genus Anthyllis. Finally, Securigera, on the basis of its flavonoid chemistry, appears to be rather remote from other members of the tribe.     

Osteoconduction,Osteogenicity, Osteoinduction,what are the fundamental properties for a smart bone substitutes

Resorbable synthetic bone graft materials are mainly calcium phosphates. These materials differ in chemical composition and physical properties, particularly in regards of osteoconduction, osteogenic and/or osteoinductive properties. Several scaffolds are characterized and compared. Results from preclinical and clinical studies are selected. Osteoconductive properties have been largely described but “osteoinductive” properties have been less explored and documented. The purpose of this paper will be to present series of data demonstrating the differences in scaffolds for bone regeneration and to explain how dissolution and, biological precipitation into the micropores occur simultaneously with osteoid and bone formation after implantation in bony and non-bony sites and support the osteogenic/osteoinductive properties.     

Demonstration of non-histone nuclear proteins in mouse myeloma. Trial comparative study

The non-histone chromosomal proteins from mouse myeloma cell line X 63-Ag 8.653 have been studied using two dimensional polyacrylamide gel electrophoresis. Our results were compared to previous analysis of other myeloma cell lines.     

On the Mechanism of Integration of Transforming Deoxyribonucleate

The characteristics of the intermediates in the reaction, between DNA and pneumococcus, that results in genetic transformation are described in so far as they have been characterized. Transformation with DNA isolated from bacteria carrying in addition to genetic markers ³²P as a radioactive label and ²H and ¹⁵N as density labels has permitted the characterization of the product of recombination between the newly introduced DNA and the DNA of a recipient bacterium. The evidence for a single strand displacement mechanism producing a hybrid structure in the DNA of the recipient bacteria is presented. Progeny of single transformants have been examined. The results of these segregation studies permit the further characterization of this hybrid product of transformation as a genetically heterozygous structure.     

CELL CYCLE KINETICS IN AN IN VITRO TUMOR MODEL

Cell cycle kinetic parameters of multicell spheroids in vitro have been estimated using thymidine labeling techniques and autoradiography. Both the mitotic index and thymidine labeling index decreased in larger spheroids, whereas the duration of the cell cycle, as determined by two independent methods utilizing labeled mitoses or labeled cells, was essentially independent of spheroid size or age. These results suggest that the tumor-like growth exhibited by spheroids is the result of a decreasing growth fraction and a large apparent cell loss, rather than a general elongation of the cell cycle.     

Spontaneous Reperfusion after In Situ Thromboembolic Stroke in Mice

Injection of thrombin into the middle cerebral artery (MCA) of mice has been proposed as a new model of thromboembolic stroke. The present study used sequential multiparametric Magnetic Resonance Imaging (MRI), including Magnetic Resonance Angiography (MRA), Diffusion-Weighted Imaging (DWI) and Perfusion-Weighted Imaging (PWI), to document MCA occlusion, PWI-DWI mismatch, and lesion development. In the first experiment, complete MCA occlusion and reproducible hypoperfusion were obtained in 85% of animals during the first hour after stroke onset. In the second experiment, 80% of animals showed partial to complete reperfusion during a three-hour follow-up. Spontaneous reperfusion thus contributed to the variability in ischemic volume in this model. The study confirmed the value of the model for evaluating new thrombolytic treatments, but calls for extended MRI follow-up at the acute stage in therapeutic studies.