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81.
Differential expression of PARK2 splice isoforms in an in vitro model of dopaminergic‐like neurons exposed to toxic insults mimicking Parkinson's disease
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Valentina La Cognata Grazia Maugeri Agata Grazia D'Amico Salvatore Saccone Concetta Federico Sebastiano Cavallaro Velia D'Agata 《Journal of cellular biochemistry》2018,119(1):1062-1073
Mutations in PARK2 (or parkin) are responsible for 50% of cases of autosomal‐recessive juvenile‐onset Parkinson's disease (PD). To date, 21 alternative splice variants of the human gene have been cloned. Yet most studies have focused on the full‐length protein, whereas the spectrum of the parkin isoforms expressed in PD has never been investigated. In this study, the role of parkin proteins in PD neurodegeneration was explored for the first time by analyzing their expression profile in an in vitro model of PD. To do so, undifferentiated and all‐trans‐retinoic‐acid (RA)‐differentiated SH‐SY5Y cells (which thereby acquire a PD‐like phenotype) were exposed to PD‐mimicking neurotoxins: 1‐methyl‐4‐phenylpyridinium (MPP+) and 6‐hydroxydopamine (6‐OHDA) are widely used in PD models, whereas carbonyl cyanide m‐chlorophenyl hydrazone (CCCP) and carbobenzoxy‐Leu‐Leu‐leucinal (MG132) interfere, respectively, with mitochondrial mitophagy and proteasomal degradation. Following treatment with each neurotoxin H1, the first parkin isoform to be cloned, was down‐regulated compared to the respective controls both in undifferentiated and RA‐differentiated cells. In contrast, the expression pattern of the minor splice isoforms varied as a function of the compound used: it was largely unchanged in both cell cultures (eg, H21‐H6, H12, XP isoform) or it showed virtually opposite alterations in undifferentiated and RA‐differentiated cells (eg, H20 and H3 isoform). This complex picture suggests that up‐ or down‐regulation may be a direct effect of toxin exposure, and that the different isoforms may exert different actions in neurodegeneration via modulation of different molecular pathways. 相似文献
82.
Body Mass Index and Treatment Outcomes in Metastatic Breast Cancer Patients Treated With Eribulin
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Maddalena Barba Laura Pizzuti Isabella Sperduti Clara Natoli Teresa Gamucci Domenico Sergi Luigi Di Lauro Luca Moscetti Fiorentino Izzo Massimo Rinaldi Lucia Mentuccia Angela Vaccaro Laura Iezzi Antonino Grassadonia Andrea Michelotti Elisabetta Landucci Letizia Perracchio Edoardo Pescarmona Franco Di Filippo Antonio Giordano Marcello Maugeri‐Saccà Patrizia Vici 《Journal of cellular physiology》2016,231(5):986-991
83.
Anthropometric,Metabolic and Molecular Determinants of Human Epidermal Growth Factor Receptor 2 Expression in Luminal B Breast Cancer
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Patrizia Vici Anna Crispo Antonio Giordano Luigi Di Lauro Francesca Sperati Irene Terrenato Laura Pizzuti Domenico Sergi Marcella Mottolese Claudio Botti Maria Grimaldi Immacolata Capasso Giuseppe D'Aiuto Maurizio Di Bonito Flaviano Di Paola Marcello Maugeri‐Saccà Maurizio Montella Maddalena Barba 《Journal of cellular physiology》2015,230(8):1708-1712
84.
85.
Agata Grazia D'Amico Grazia Maugeri Daniela Rasà Concetta Federico Salvatore Saccone Francesca Lazzara Annamaria Fidilio Filippo Drago Claudio Bucolo Velia D'Agata 《Journal of cellular physiology》2019,234(4):5230-5240
Diabetic retinopathy (DR) is a common microvascular complication of diabetes. Prolonged hyperglycemia stimulates inflammatory pathway characterized by the release of some cytokines leading to the impairment of blood retinal barrier (BRB). NAP exerts a protective effect in various eye diseases, including DR. So far, the role of NAP in the modulation of inflammatory event during early phase of this pathology has not been investigated yet. In the current study, we have studied the retinal protective effect of NAP, injected into the eye, in diabetic rats. NAP treatment exerts a dual effect downregulating interleukin (IL)-1β and its related receptors and upregulating IL-1Ra expression. We have also tested the role of this peptide in human retinal epithelial cells (ARPE19) cultured on a semipermeable support and exposed to hyperglycemic–inflammatory insult, representing a in vitro model of diabetic macular edema, a clinical manifestation of DR. The results have shown that NAP prevents outer BRB impairment by upregulating the tight junctions. In conclusion, deepened characterization of NAP action mechanism on hyperglycemic–inflammatory damage may be useful to develop a new strategy to prevent retinal damage during DR. 相似文献
86.
A. Maugeri I. Chiarelli E. Di Paolo P. L. Mattiuz B. Porfirio C. Carcassi L. Contu 《International Journal of Anthropology》1999,14(2-3):191-202
We typed the Sardinian population at the D1S80 VNTR locus. Nineteen alleles were detected in a sample of 92 unrelated individuals,
allele frequency distribution showing a modal pattern mostly in agreement with other Caucasoid populations. A high degree
of heterozygosity (observed value=80.4%) was present. Goodness-of-fit tests demonstrated no departure from Hardy-Weinberg
expectations. Data regarding heterozygosity, number of alleles and singletons appeared in accordance with the IAM mutation-drift
equilibrium model and showed no evidence of hidden substructuring.
Allele 34 exhibited in Sardinians the highest frequency never observed in Caucasians. Nonetheless, the comparison with other
European populations did not disclose Sardinian genetic peculiarity. Indeed, measures of genetic divergence among Europeans
demonstrated definitely smaller values at the D1S80 locus in comparison with those calculated over a high number of (pre-DNA)
polymorphic loci. High mutation rate and selective neutrality typical of VNTRs could account for the observed moderate genetic
divergence. Isolation and genetic drift, on the other hand, may have determined certain deviations in allele frequency distribution,
as occurred to allele 34 in the Sardinian population. 相似文献
87.
In vitro synthesis of oligosaccharides by acceptor reaction of dextransucrase from Leuconostoc mesenteroides 总被引:1,自引:0,他引:1
Glucose was used as acceptor to obtain small chain oligossaccharides from sucrose using dextransucrase from Leuconostoc mesenteroides NRRL B-512F. Better conditions for the synthesis of the oligosaccharides were obtained using experimental design and response surface methodology. Yield of oligosaccharides was increased from 5% to 45% following an increase in both sucrose and glucose/sucrose concentrations, from 58 g/l to 142 g/l and from 0.02 to 0.18, respectively. Molecular weight increased from 2800 to 4500 daltons with a temperature shifting from 10°C to 30°C. © Rapid Science Ltd. 1998 相似文献
88.
M.L.C. Acosta Pomar G. Caruso T.L. Maugeri R. Scarfò & R. Zaccone 《Journal of applied microbiology》1998,84(4):493-500
By using two polyclonal antisera against WH 7803 strain (Synechococcus sp.) and WH 5701 strain (Synechococcus bacillaris) it is possible to detect and to enumerate cells of the two cyanobacterial serogroups. The immunofluorescence technique was used to study the distribution of the two serogroups in the estuarine, coastal and upwelling waters of the Mediterranean Sea surrounding Messina. In the estuarine waters of the Alcantara River (Ionian Sea), the WH 7803 serogroup was present at a concentration in the order of 102 cells ml−1 and the WH 5701 serogroup at a concentration of 5·5 × 102 cellsml−1 . In the coastal waters of Messina, where urban and industrial wastes are usuallydumped, the concentration of total phycoerythrin- Synechococcus ranged from 1·3 × 102 to 4·1 × 103 cells ml−1 ; the WH 7803 serogroup accounted for 50–94% of the totalpopulation in Ionian stations, whereas the WH 5701 serogroup ranged from1·4 × 101 to 6·7 × 102 cells ml−1 . In the upwelling area (Straits of Messina) bothserogroups were found. Vertical distribution of two Synechococcus strains had anopposite trend and their concentrations were of the order of 101 –102 cells ml−1 . Theuse of the Scan laser system allows both autofluorescent and labelled organismsto be distinguished in a preparation for optical microscopy. It also allows false-positivecells to be distinguished. 相似文献
89.
90.
Maugeri TL Irrera GP Lentini V Carbone M Fera MT Gugliandolo C 《The new microbiologica》2005,28(2):177-182
Seawater and plankton samples from the Straits of Messina, Italy, were analysed to confirm the occurrence of potentially pathogenic Arcobacter butzleri, A. cryaerophilus and A. skirrowii. Both classical cultural methods and molecular techniques were used as confirmative steps of the growth in enrichment broth to enumerate and differentiate these bacteria. Only A. butzleri was isolated from seawater and plankton samples and was more abundant when associated with plankton than free-living. A. cryaerophilus was occasionally detected by PCR assay from environmental samples. The PCR procedure, used in a combined method, was useful in enumerating Arcobacter spp. in marine environment. 相似文献