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排序方式: 共有114条查询结果,搜索用时 156 毫秒
111.
M.T. Fera C. Gugliandolo V. Lentini A. Favaloro D. Bonanno E. La Camera T.L. Maugeri 《Letters in applied microbiology》2010,50(1):65-70
Aim: To evaluate the reliability of culture-independent methods in comparison with culture-dependent ones for the detection of Arcobacter spp. in estuarine waters of Southern Italy.
Methods and Results: PCR and fluorescent in situ hybridization (FISH) procedures were used to detect arcobacters directly in water samples and after enrichment cultures. The samples totally were positive by molecular methods (PCR and FISH) but only 75% were culture positive, confirming the limitation of these latter to detect Arcobacter spp. in natural samples. Culturable arcobacters were retrieved in all times except in July, and isolated species were ascribed only to Arcobacter cryaerophilus .
Conclusions: Culturable and nonculturable forms of Arcobacter in the estuarine environment were present. PCR assays were more sensitive than traditional culture in detecting Arcobacter butzleri and A. cryaerophilus . FISH comparatively to PCR technique may provide information about cell morphology and viability of single cells.
Significance and Impact of the Study: Our investigation indicates the existence of an environmental reservoir of potential pathogenic arcobacters in an estuarine Italian area, which may survive under a viable but not culturable state. 相似文献
Methods and Results: PCR and fluorescent in situ hybridization (FISH) procedures were used to detect arcobacters directly in water samples and after enrichment cultures. The samples totally were positive by molecular methods (PCR and FISH) but only 75% were culture positive, confirming the limitation of these latter to detect Arcobacter spp. in natural samples. Culturable arcobacters were retrieved in all times except in July, and isolated species were ascribed only to Arcobacter cryaerophilus .
Conclusions: Culturable and nonculturable forms of Arcobacter in the estuarine environment were present. PCR assays were more sensitive than traditional culture in detecting Arcobacter butzleri and A. cryaerophilus . FISH comparatively to PCR technique may provide information about cell morphology and viability of single cells.
Significance and Impact of the Study: Our investigation indicates the existence of an environmental reservoir of potential pathogenic arcobacters in an estuarine Italian area, which may survive under a viable but not culturable state. 相似文献
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114.
Marcus B. S. Forte Marcio A. Mazutti Francisco Maugeri Filho Maria Isabel Rodrigues 《Biotechnology and Bioprocess Engineering》2011,16(6):1223-1230
This work investigates the adsorption of clavulanic acid using natural six cationic forms (Na+, Ca+2, Ba+2, Sr2+, K+, and Mg2+) of the X and NZ zeolites in a stirred tank reactor since the separation is an important step of the biomolecule production.
A mathematical model was proposed taking into account the transport of CA molecules from the liquid phase to the surface of
the adsorbent and after diffusion into the particles. The estimated kinetic and mass transfer parameters were used to evaluate
adsorption rates and mass transfer resistances involved in the separation of clavulanic acid from the broth. It has been shown
that mass-transfer phenomena were a limiting step in the clavulanic acid adsorption process and that the adsorption rate should
be considered to evaluate the system. Amongst the materials, the synthetic zeolite NaX was selected as the most appropriate
material to separate clavulanic acid because this material presented the highest values for the observed reaction rate, compensating
for the external mass transfer resistance. Modeling and simulation of clavulanic acid purification using the zeolite NaX showed
a satisfactory fitting of experimental data. The model was used to simulate the process and it was evaluated for its technical
and economical viability by comparisons considering the influence of the solid:liquid ratio on the adsorption equilibrium
time and on the hydrolysed mass of biomolecule. 相似文献