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51.
Val rie B caert Maude Beaulieu Jos e Gagnon Richard Villemur Louise Deschê nes R jean Samson 《Bioremediation Journal》2001,5(3):183-192
An indigenous microbial consortium capable of degrading pentachlorophenol (PCP) and petroleum hydrocarbons (C10-C50) was produced from a soil contaminated with wood-preserving oil. Two 10-L stainless steel soil slurry (10% w/v) bioreactors were operated in fed-batch mode. To verify the growth and efficiency of PCP degraders in the presence of other contaminants, one bioreactor was fed with a PCP-based wood-preserving mixture (WPM) and a second reactor was fed with technical-grade NaPCP. During the 90-day period of activation, PCP, C10-C50, Cl-, pH, and dissolved oxygen levels were monitored. The microbial community was monitored using specific most probably number (MPN) bacterial counts and mineralization tests. PCP degradation rates increased similarly in both reactors, from 19 to 132 mg/L-d in the NaPCP reactor, and from 41 to 112 mg/L-d in the WPM reactor. Contaminant loss calculations showed that 99.5% of PCP and 92.5% of C10-C50 added to the WPM reactor were biodegraded. It also revealed that 83% of polychlorinated dioxins and furans were removed. PCP-degrading bacteria increased from 7×102 to 1.6×106 bacteria/mL in both reactors, and petroleum hydrocarbon degraders increased from 1.7×102 to 3.4×108 bacteria/mL in the WPM reactor, indicating that the activity of PCP degraders was not inhibited by the presence of microorganisms growing on petroleum hydrocarbons. 相似文献
52.
Yann Pecrix Luis Buendia Charlotte Penouilh‐Suzette Maude Marchaux Ludovic Legrand Olivier Bouchez David Rengel Jrme Gouzy Ludovic Cottret Felicity Vear Laurence Godiard 《The Plant journal : for cell and molecular biology》2019,97(4):730-748
Over the last 40 years, new sunflower downy mildew isolates (Plasmopara halstedii) have overcome major gene resistances in sunflower, requiring the identification of additional and possibly more durable broad‐spectrum resistances. Here, 354 RXLR effectors defined in silico from our new genomic data were classified in a network of 40 connected components sharing conserved protein domains. Among 205 RXLR effector genes encoding conserved proteins in 17 P. halstedii pathotypes of varying virulence, we selected 30 effectors that were expressed during plant infection as potentially essential genes to target broad‐spectrum resistance in sunflower. The transient expression of the 30 core effectors in sunflower and in Nicotiana benthamiana leaves revealed a wide diversity of targeted subcellular compartments, including organelles not so far shown to be targeted by oomycete effectors such as chloroplasts and processing bodies. More than half of the 30 core effectors were able to suppress pattern‐triggered immunity in N. benthamiana, and five of these induced hypersensitive responses (HR) in sunflower broad‐spectrum resistant lines. HR triggered by PhRXLRC01 co‐segregated with Pl22 resistance in F3 populations and both traits localized in 1.7 Mb on chromosome 13 of the sunflower genome. Pl22 resistance was physically mapped on the sunflower genome recently sequenced, unlike all the other downy mildew resistances published so far. PhRXLRC01 and Pl22 are proposed as an avirulence/resistance gene couple not previously described in sunflower. Core effector recognition is a successful strategy to accelerate broad‐spectrum resistance gene identification in complex crop genomes such as sunflower. 相似文献
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54.
Maude?Pauly Chantal?Akoua-Koffi Nanina?Buchwald Grit?Schubert Sabrina?Weiss Emmanuel?Couacy-Hymann Augustin?Etile?Anoh Arsène?Mossoun Sébastien?Calvignac-Spencer Siv?Aina?Leendertz Fabian?H.?LeendertzEmail author Bernhard?EhlersEmail author 《EcoHealth》2015,12(3):441-452
The Taï region in Western Côte d`Ivoire is characterized by extensive overlap of human and animal habitats. This could influence patterns of adenovirus transmission between humans and domestic animals. Fecal samples from humans and various domestic animals were tested for the presence of adenoviruses by PCR. Phylogenetic and species delineation analyses were performed to further characterize the adenoviruses circulating in the region and to identify potential cross-species transmission events. Among domestic animals, adenovirus shedding was frequent (21.6% of domestic mammals and 41.5% of chickens) and the detected strains were highly diverse, several of them representing novel types. Although no evidence for zoonotic transmission of animal adenovirus was obtained, the present study provides concordant evidence in favor of common cross-species transmission of adenoviruses between different animal species and first indications for adenovirus transmission from humans to animals. These findings underline the thus far underestimated importance of reverse zoonotic transmission of viruses and of the role of domestic animals as pathogen reservoirs, “bridge species,” or intermediate hosts. 相似文献
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56.
Béchet M Caradec T Hussein W Abderrahmani A Chollet M Leclère V Dubois T Lereclus D Pupin M Jacques P 《Applied microbiology and biotechnology》2012,95(3):593-600
A new family of lipopeptides produced by Bacillus thuringiensis, the kurstakins, was discovered in 2000 and considered as a biomarker of this species. Kurstakins are lipoheptapeptides displaying antifungal activities against Stachybotrys charatum. Recently, the biosynthesis mechanism, the regulation of this biosynthesis and the potential new properties of kurstakins were described in the literature. In addition, kurstakins were also detected in other species belonging to Bacillus genus such as Bacillus cereus. This mini-review gathers all the information about these promising bioactive molecules. 相似文献
57.
Tjaden B Goodwin SS Opdyke JA Guillier M Fu DX Gottesman S Storz G 《Nucleic acids research》2006,34(9):2791-2802
58.
Genomic instability has long been considered a key factor in tumorigenesis. Recent evidence suggests that DNA damage may be widespread in early pre-neoplastic states, with deregulation of cyclin-dependent kinase (Cdk) activity a driving force. Increased Cdk activity may critically reduce licensing of origins of DNA replication, drive re-replication, or mediate overexpression of checkpoint proteins, inducing deleterious cell cycle delay. Conversely, inhibition of Cdk activity may compromise replication efficiency, expression of checkpoint proteins, or activation of DNA repair proteins. These vital functions point to the impact of Cdk activity on the stability of the genome. Insight into these pathways may improve our understanding of tumorigenesis and lead to more rational cancer therapies. 相似文献
59.
Brown hyaena scent marks were tested for their potential to provide DNA suitable for PCR. Up to 100% of scent marks were successfully
amplified. The approach can potentially be adapted to other species with similar pasting behaviour and scent mark morphology,
or to samples that are environmentally exposed/degraded/inhibitor-rich or where there are very limited amounts of sample. 相似文献
60.
Aulus EAD Barbosa Érika VS Albuquerque Maria CM Silva Djair SL Souza Osmundo B Oliveira-Neto Arnubio Valencia Thales L Rocha Maria F Grossi-de-Sa 《BMC biotechnology》2010,10(1):44