全文获取类型
收费全文 | 300篇 |
免费 | 28篇 |
专业分类
328篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 7篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 19篇 |
2014年 | 17篇 |
2013年 | 21篇 |
2012年 | 23篇 |
2011年 | 14篇 |
2010年 | 13篇 |
2009年 | 10篇 |
2008年 | 14篇 |
2007年 | 11篇 |
2006年 | 10篇 |
2005年 | 10篇 |
2004年 | 8篇 |
2003年 | 11篇 |
2002年 | 8篇 |
2001年 | 12篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1996年 | 3篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1980年 | 2篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1970年 | 2篇 |
1943年 | 3篇 |
1940年 | 2篇 |
1932年 | 2篇 |
1928年 | 3篇 |
1923年 | 2篇 |
1918年 | 2篇 |
1913年 | 2篇 |
1899年 | 1篇 |
1897年 | 1篇 |
排序方式: 共有328条查询结果,搜索用时 15 毫秒
271.
Isabelle Aubin Franoise Cardou Laura Boisvert‐Marsh Eric Garnier Manuella Strukelj Alison D. Munson 《植被学杂志》2020,31(3):509-517
Ecologists are increasingly asking large‐scale and/or broad‐scope questions that require vast datasets. In response, various top‐down efforts and incentives have been implemented to encourage data sharing and integration. However, despite general consensus on the critical need for more open ecological data, several roadblocks still discourage compliance and participation in these projects; as a result, ecological data remain largely unavailable. Grassroots initiatives (i.e. efforts initiated and led by cohesive groups of scientists focused on specific goals) have thus far been overlooked as a powerful means to meet these challenges. These bottom‐up collaborative data integration projects can play a crucial role in making high quality datasets available because they tackle the heterogeneity of ecological data at a scale where it is still manageable, all the while offering the support and structure to do so. These initiatives foster best practices in data management and provide tangible rewards to researchers who choose to invest time in sound data stewardship. By maintaining proximity between data generators and data users, grassroots initiatives improve data interpretation and ensure high‐quality data integration while providing fair acknowledgement to data generators. We encourage researchers to formalize existing collaborations and to engage in local activities that improve the availability and distribution of ecological data. By fostering communication and interaction among scientists, we are convinced that grassroots initiatives can significantly support the development of global‐scale data repositories. In doing so, these projects help address important ecological questions and support policy decisions. 相似文献
272.
273.
Wonjung Kim Fran?ois Peaudecerf Maude W. Baldwin John W. M. Bush 《Proceedings. Biological sciences / The Royal Society》2012,279(1749):4990-4996
We present the results of a combined experimental and theoretical investigation of the dynamics of drinking in ruby-throated hummingbirds. In vivo observations reveal elastocapillary deformation of the hummingbird''s tongue and capillary suction along its length. By developing a theoretical model for the hummingbird''s drinking process, we investigate how the elastocapillarity affects the energy intake rate of the bird and how its open tongue geometry reduces resistance to nectar uptake. We note that the tongue flexibility is beneficial for accessing, transporting and unloading the nectar. We demonstrate that the hummingbird can attain the fastest nectar uptake when its tongue is roughly semicircular. Finally, we assess the relative importance of capillary suction and a recently proposed fluid trapping mechanism, and conclude that the former is important in many natural settings. 相似文献
274.
doi: 10.1111/j.1741‐2358.2011.00599.x A prospective study on oral microbial flora and related variables in dentate dependent elderly residents Objective: Oral microorganisms and related variables were followed over a 1‐year period in dentate dependent elderly. Background: For nursing personnel, oral hygiene for the dependent elderly is demanding. It requires good knowledge of how to carry out preventive oral health measures and to judge when help is needed. Previous studies indicate low levels of knowledge, which might be reflected in the oral health of dentate residents. Material and methods: Twenty‐one subjects participated at baseline and 14 one year later. Microbial samples were analysed using cultivation technique. The labial minor gland secretion rate was measured using the Periotron® method, and oral clinical data were registered. Results: Low labial mucosal flow rates were seen for 95% of the participants. At baseline, visibly thick plaque was seen in 48% and 1 year later in 57%. Both frequency and number of bacteria associated with caries and frequency of microorganisms associated with oral soft tissue infections were high. One year later, a decrease in bacteria associated with good oral health and a tendency to an increase in aciduric microorganisms were seen. Conclusion: Poor oral health, tending to further deteriorate over time, advocates regular professional supplementary support when elderly dentate dependent residents need assistance with their oral care. 相似文献
275.
Fer M Préchoux A Leroy A Sassi JF Lahaye M Boisset C Nyvall-Collén P Helbert W 《Journal of microbiological methods》2012,89(3):222-229
Polysaccharides are the most abundant and the most diverse renewable materials found on earth. Due to the stereochemical variability of carbohydrates, polysaccharide-degrading enzymes - i.e. glycoside hydrolases and polysaccharide lyases - are essential tools for resolving the structure of these complex macromolecules. The exponential increase of genomic and metagenomic data contrasts sharply with the low number of proteins that have ascribed functions. To help fill this gap, we designed and implemented a medium-throughput profiling method to screen for polysaccharide-degrading enzymes in crude bacterial extracts. Our strategy was based on a series of filtrations, which are absolutely necessary to eliminate any reducing sugars not directly generated by enzyme degradation. In contrast with other protocols already available in the literature, our method can be applied to any panel of polysaccharides having known and unknown structures because no chemical modifications are required. We applied this approach to screen for enzymes that occur in Pseudoalteromonas carrageenovora grown in two culture conditions. 相似文献
276.
Boisvert FM Ahmad Y Gierliński M Charrière F Lamont D Scott M Barton G Lamond AI 《Molecular & cellular proteomics : MCP》2012,11(3):M111.011429
Measuring the properties of endogenous cell proteins, such as expression level, subcellular localization, and turnover rates, on a whole proteome level remains a major challenge in the postgenome era. Quantitative methods for measuring mRNA expression do not reliably predict corresponding protein levels and provide little or no information on other protein properties. Here we describe a combined pulse-labeling, spatial proteomics and data analysis strategy to characterize the expression, localization, synthesis, degradation, and turnover rates of endogenously expressed, untagged human proteins in different subcellular compartments. Using quantitative mass spectrometry and stable isotope labeling with amino acids in cell culture, a total of 80,098 peptides from 8,041 HeLa proteins were quantified, and their spatial distribution between the cytoplasm, nucleus and nucleolus determined and visualized using specialized software tools developed in PepTracker. Using information from ion intensities and rates of change in isotope ratios, protein abundance levels and protein synthesis, degradation and turnover rates were calculated for the whole cell and for the respective cytoplasmic, nuclear, and nucleolar compartments. Expression levels of endogenous HeLa proteins varied by up to seven orders of magnitude. The average turnover rate for HeLa proteins was ~20 h. Turnover rate did not correlate with either molecular weight or net charge, but did correlate with abundance, with highly abundant proteins showing longer than average half-lives. Fast turnover proteins had overall a higher frequency of PEST motifs than slow turnover proteins but no general correlation was observed between amino or carboxyl terminal amino acid identities and turnover rates. A subset of proteins was identified that exist in pools with different turnover rates depending on their subcellular localization. This strongly correlated with subunits of large, multiprotein complexes, suggesting a general mechanism whereby their assembly is controlled in a different subcellular location to their main site of function. 相似文献
277.
278.
279.
280.