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991.
B E Matthews 《Parasitology》1972,65(3):457-467
992.
Rong Jian Lu Catherine J. Mader Stephen E. Schneider Nicolai Tvermoes Myung‐Choi Kang John J. Dwyer Karen L. Wilson Thomas J. Matthews Mary K. Delmedico Brian Bray 《Journal of peptide science》2010,16(9):465-472
A helical, prehairpin trimer covering the majority of the HR1 region of human immunodeficiency virus gp41 was achieved by chemically coupling three identical 51 amino acid peptides. A 1,3,5‐tris(aminomethyl)‐2,4,6‐triethylbenzene pinwheel ‘cap’ was used to trimerize the peptides by taking advantage of the unique property of triacyl fluoride and orthogonal protection and deprotection. The resulting protein is fully helical, highly thermostable and soluble. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
993.
Beibei Zhang Baojuan Wang Peng Wang Zhengyu Cao Enqi Huang Jiasheng Hao Antony M. Dean Guoping Zhu 《Biochimie》2009,91(11-12):1405-1410
Isocitrate dehydrogenase (IDH) is one of the key enzymes in the citric acid cycle, which involves in providing energy and biosynthetic precursors for metabolism. Here, we report for the first time the enzymatic characterization of a monomeric NADP+-dependent IDH from Streptomyces lividans TK54 (SlIDH). The icd gene (GenBank database accession number EU661252) encoding IDH was cloned and overexpressed in Escherichia coli. The molecular mass of SlIDH was about 80 kDa, typical of a monomeric NADP-IDH, and showed high amino acid sequence identity with known monomeric IDHs. The optimal activity of the 6His-tagged SlIDH was found at pH values 8.5 (Mn2+) and 9.0 (Mg2+), and the optimal temperature was around 46 °C. Heat-inactivation studies showed that about 50% SlIDH activity was preserved at 38 °C after 20 min of incubation. The recombinant SlIDH displayed a 62,000-fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+, and a 85,000-fold greater specificity for NADP+ than NAD+ with Mg2+. Therefore, SlIDH is a divalent cation-dependent monomeric IDH with remarkably high coenzyme preference for NADP+. 相似文献
994.
Iwona E. Weidlich Thomas Dexheimer Christophe Marchand Smitha Antony Yves Pommier Marc C. Nicklaus 《Bioorganic & medicinal chemistry》2010,18(1):182-189
Human tyrosyl-DNA phosphodiesterase (hTdp1) inhibitors have become a major area of drug research and structure-based design since they have been shown to work synergistically with camptothecin (CPT) and selectively in cancer cells. The pharmacophore features of 14 hTdp1 inhibitors were used as a filter to screen the ChemNavigator iResearch Library of about 27 million purchasable samples. Docking of the inhibitors and hits obtained from virtual screening was performed into a structural model of hTdp1 based on a high resolution X-ray crystal structure of human Tdp1 in complex with vanadate, DNA and a human topoisomerase I (TopI)-derived peptide (PDB code: 1NOP). A total of 46 compounds matching the three-dimensional arrangement of the pharmacophoric features were assayed. Using a high-throughput screening assay, we have identified an 1H-indol-3-yl-acetic acid derivative as a potent Tdp1 inhibitor with an IC50 value of 7.94 μM. The obtained novel chemotype may provide a new scaffold for developing inhibitors of Tdp1. 相似文献
995.
996.
M A McAleer M A Breen N L White N Matthews 《The Journal of biological chemistry》1999,274(33):23541-23548
997.
Nuclear extracts of the true slime mold, Physarum polycephalum, show protein histidine kinase activity towards exogenous histones [(1985) J. Biol. Chem. 260, 16106-16113]. Physarum microplasmodia were labeled with [32P]phosphate in vivo and two basic proteins containing alkali-stable phosphate were detected. The labeled proteins comigrated with Physarum histones H1 (approximately) and H2A and phosphoamino acid analysis showed that each protein contained [32P]-phosphohistidine. The H2A-like protein was also labeled in isolated nuclei incubated with [35S]thio-ATP. We conclude that some Physarum nuclear proteins contain phosphohistidine. 相似文献
998.
Frequent polymerase errors observed in a restricted area of clones derived from the attachment (G) protein gene of respiratory syncytial virus. 总被引:2,自引:1,他引:1 下载免费PDF全文
Sequence analysis of a large number of clones derived from the carboxy-terminal one-third of the attachment (G) protein gene of subgroup A respiratory syncytial viruses revealed a region very prone to polymerase errors which resulted mainly in frameshifts because of the insertion or deletion of adenosine residues in some but not all runs of such residues. Such mutations were detected in 14% of clones derived from mRNA, 58% of clones derived from genomic-sense RNA, and 50% of clones derived from in vitro-transcribed RNA. This phenomenon appears to be dependent on the template sequence. 相似文献
999.
1000.