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排序方式: 共有134条查询结果,搜索用时 15 毫秒
121.
MicroRNAs (miRNAs) and related polymorphisms have been implicated in the susceptibility to oesophageal squamous cell carcinoma (OSCC). In our study, three miRNA-related SNPs: rs6505162 A>C (pre-miRNA of miR-423), rs213210 A>G (3’UTR of miR-219-1) and rs7372209 C>T (5’UTR of miR-26a-1) were investigated in the Black and Mixed Ancestry population groups in South Africa. The potential cumulative effects of these SNPs, as well as gene-environment interactions were also analysed. In Blacks, rs6505162 A>C was associated with OSCC under dominant, additive and recessive models with odds ratios (ORs) 1.353, 1.404, and 2.858, respectively. This locus showed very strong interactions with smoke inhalation from burning wood or charcoal used for heating and cooking in very poorly ventilated areas (OR(GE)=7.855, P(GE)=9.17*10-10 in the Black group). Furthermore, the miR-423-3p level was 1.39 fold up-regulated in tumour tissues compared to the adjacent normal tissue (paired t-test P value 0.0087). SNP-SNP interaction between rs2132210 and rs7372209 was found in both Black and Mixed Ancestry subjects. The AArs213210-CTrs7372209 genotype had a protective effect on OSCC risk (in the Black, OR=0.229, P=0.012; and the Mixed Ancestry groups, OR=0.230, P=0.00014). This study is the first to link SNPs in miR-423 together with environmental smoke exposure to risk for developing OSCC. 相似文献
122.
Spatial coherence resonance in excitable biochemical media induced by internal noise 总被引:2,自引:1,他引:1
We show that in a spatially extended excitable medium, presently modelled with diffusively coupled FitzHugh-Nagumo neurons, internal stochasticity is able to extract a characteristic spatial frequency of waves on the spatial grid. Internal noise is introduced via a stochastic simulation method and is the only agent acting on the system. Remarkably, the spatial periodicity is best pronounced at an intermediate level of internal stochasticity. Thus, the reported phenomenon is an observation of internal noise spatial coherence resonance in excitable biochemical media. 相似文献
123.
Scott Peterman Eric E. Niederkofler David A. Phillips Bryan Krastins Urban A. Kiernan Kemmons A. Tubbs Dobrin Nedelkov Amol Prakash Maryann S. Vogelsang Tara Schoeder Lewis Couchman David R. Taylor Cajetan F. Moniz Gouri Vadali Gregory Byram Mary F. Lopez 《Proteomics》2014,14(12):1445-1456
The detection and quantification of insulin and its therapeutic analogs is important for medical, sports doping, and forensic applications. Synthetic variants contain slight sequence variations to affect bioavailability. To reduce sample handling bias, a universal extraction method is required for simultaneous extraction of endogenous and variant insulins with subsequent targeted quantification by LC‐MS. A mass spectrometric immunoassay (MSIA), a multiplexed assay for intact insulin and its analogues that couples immunoenrichment with high resolution and accurate mass (HR/AM) spectrometric detection across the clinical range is presented in this report. The assay is sensitive, selective, semi‐automated and can potentially be applied to detect new insulin isoforms allowing their further incorporation into second or third generation assays. 相似文献
124.
Nucleus pulposus repair with cultured autologous elastic cartilage derived chondrocytes 总被引:14,自引:0,他引:14
Gorensek M Jaksimović C Kregar-Velikonja N Gorensek M Knezevic M Jeras M Pavlovcic V Cör A 《Cellular & molecular biology letters》2004,9(2):363-373
Low back pain is one of the most common medical conditions in the Western world. Disc degeneration, an inevitable process of ageing, is one of the major causes of low back pain. Autologous chondrocyte transplantation (ACT) is an increasingly popular method of addressing pathological disorders of cartilage. The purpose of our study was to determine whether autologous chondrocytes from elastic cartilage could survive and synthesise a cartilage specific matrix in the intervertebral disc of rabbits. Sixteen lumbar intervertebral discs (IVD) of New Zealand White rabbits were analysed. In 6 IVD, the nucleus pulposus was evacuated and replaced with tissue engineered autologous chondrocytes from auricular cartilage. In the second group, only the nucleus pulposus was evacuated from 6 IVD, with no chondrocytes implantation. Four non-operated IVD were used as a control. Six months after the operation, the animals were euthanized and the IVD were analysed histologically. Autologous cartilage implants were well tolerated by the host for up to six months in vivo. There was only hyaline-like cartilage in the place of the nucleus pulposus. We could not detect any elastic fibres in the new cartilage matrix. In IVD from which only the nucleus pulposus was evacuated and no chondrocytes were implanted, just fibrous tissue was found instead of nucleus pulposus. The overall histological analysis of new cartilage produced after implantation in our study confirmed the hypothesis that ACT from auricular cartilage can be implanted into the IVD instead of the nucleus pulposus and that a significant percentage of implanted chondrocytes survive and produce hyaline-like cartilage. 相似文献
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127.
M Karl G Metzner W Franke H Vogelsang 《Folia haematologica (Leipzig, Germany : 1928)》1983,110(1):9-23
A great pool procedure for producing human transfer factor preparations from buffy-coats of stored whole blood which has not been used up till now is described. From an initial tool size of about 1,500 units of whole blood the procedure represented makes it possible to gain higher amounts of uniform and in their immunological efficacy in vitro standardizable preparations for controlled long-term therapy studies. By means of various immunological in vitro tests, stimulating as well as suppressing activities of the charges obtained could be identified. Starting from the differentiated immunological effects of various charges of the transfer factor on T-cell populations in vitro, the possibility of influencing T-cell subpopulations through well-conceived therapeutic measures by a transfer factor is discussed. 相似文献
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129.
Pernilla Östlund Kalle Kilk Maria Lindgren Mattias Hällbrink Yang Jiang Metka Budihna Katarina Cerne Aljosa Bavec Claes-Göran Östenson Matjaz Zorko Ülo Langel 《International journal of peptide research and therapeutics》2005,11(4):237-247
Cell-penetrating peptides have proven themselves as valuable vectors for intracellular delivery. Relatively little is known
about the frequency of cell-penetrating sequences in native proteins and their functional role. By computational comparison
of peptide sequences, we recently predicted that intracellular loops of G-protein coupled receptors (GPCR) have high probability
for occurrence of cell-penetrating motifs. Since the loops are also receptor and G-protein interaction sites, we postulated
that the short cell-penetrating peptides, derived from GPCR, when applied extracellularly can pass the membrane and modulate
G-protein activity similarly to parent receptor proteins. Two model systems were analyzed as proofs of the principle. A peptide
based on the C-terminal intracellular sequence of the rat angiotensin receptor (AT1AR) is shown to internalize into live cells
and elicit blood vessel contraction even in the presence of AT1AR antagonist Sar1-Thr8-angiotensin II. The peptide interacts with the same selectivity towards G-protein subtypes as agonist-activated AT1AR and
blockade of phospholipase C abolishes its effect. Another cell-penetrating peptide, G53-2 derived from human glucagon-like
peptide receptor (GLP-1R) is shown to induce insulin release from isolated pancreatic islets. The mechanism was again found
to be shared with the original GLP-1R, namely G11-mediated inositol 1,4,5-triphosphate release pathway. These data reveal a novel possibility to mimic the effects of signalling
transmembrane proteins by application of shorter peptide fragments. 相似文献
130.