全文获取类型
收费全文 | 102篇 |
免费 | 14篇 |
出版年
2021年 | 2篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 11篇 |
2013年 | 3篇 |
2012年 | 3篇 |
2011年 | 6篇 |
2009年 | 5篇 |
2008年 | 5篇 |
2007年 | 3篇 |
2006年 | 4篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 1篇 |
2002年 | 4篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1991年 | 2篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1965年 | 3篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1959年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有116条查询结果,搜索用时 31 毫秒
21.
Gerd Meyer zu H?rste Steffen Cordes Anne K. Mausberg Alla L. Zozulya Carsten Wessig Tim Sparwasser Christian Mathys Heinz Wiendl Hans-Peter Hartung Bernd C. Kieseier 《PloS one》2014,9(10)
Inflammatory neuropathies represent disabling human autoimmune disorders with considerable disease variability. Animal models provide insights into defined aspects of their disease pathogenesis. Forkhead box P3 (FoxP3)+ regulatory T lymphocytes (Treg) are anti-inflammatory cells that maintain immune tolerance and counteract tissue damage in a variety of immune-mediated disorders. Dysfunction or a reduced frequency of Tregs have been associated with different human autoimmune disorders. We here analyzed the functional relevance of Tregs in determining disease manifestation and severity in murine models of autoimmune neuropathies. We took advantage of the DEREG mouse system allowing depletion of Treg with high specificity as well as anti-CD25 directed antibodies to deplete Tregs in mice in actively induced experimental autoimmune neuritis (EAN). Furthermore antibody-depletion was performed in an adoptive transfer model of chronic neuritis. Early Treg depletion increased clinical EAN severity both in active and adoptive transfer chronic neuritis. This was accompanied by increased proliferation of myelin specific T cells and histological signs of peripheral nerve inflammation. Late stage Treg depletion after initial disease manifestation however did not exacerbate inflammatory neuropathy symptoms further. We conclude that Tregs determine disease severity in experimental autoimmune neuropathies during the initial priming phase, but have no major disease modifying function after disease manifestation. Potential future therapeutic approaches targeting Tregs should thus be performed early in inflammatory neuropathies. 相似文献
22.
Asexual embryos of Theobroma cacao L. cultured in a liquid medium on a rotary drum apparatus were induced to produce storage lipids, anthocyanin, and alkaloids by increasing concentration of sucrose but not glucose, and only slightly by fructose or fructose + glucose at the same osmolarity. Glucose combined with sucrose or alternating with sucrose reduced lipid concentration as compared to sucrose alone. The concomitant development of lipids, anthocyanin, and alkaloids suggests that sucrose at high concentration regulates the initiation of embryo development. 相似文献
23.
Roots as an enhancing factor for the production of artemisinin in shoot cultures of Artemisia annua 总被引:2,自引:0,他引:2
Artemisinin was produced in differentiated shoot cultures of Artemisia annua L. but was undetected in callus or cell cultures. The growth regulators benzyladenine, kinetin, chlormequat, and daminozide, at concentrations which severely reduced rooting, reduced artemisinin production. A highly significant correlation (1% level) was observed between shoot artemisinin content and number of roots (r=0.775**), but shoot number and artemisinin content were unrelated (r=-0.198). Benzyladenine increased shoot proliferation at 0.5 and 5.0 M, but decreased root production at 0.5, 5.0, and 50 M. The highest levels of artemisinin production (0.287% DW) were obtained in hormone-free medium when root production was maximized. Removal of roots from shoots cultured in hormone-free liquid medium reduced shoot artemisinin by 53% and shoot arteannuin B by 60%. Neither artemisinin, arteannuin B, or artemisinic acid were detected from roots developed in semi-solid or liquid medium.Abbreviations BA
benzyladenine
- CCC
chlormequat
- DW
dry weight
- FW
fresh weight
- GA3
gibberellic acid
- GC/MS
gas chromatography/mass spectrometry
- HPLC-EC
high-performance liquid chromatography with electrochemical detection
- MS
Murashige & Skoog basal medium
- 2,4-d
2,4-dichlorophenoxyacetic acid
Journal paper no. 14558 of Purdue Agricultural Research Progress 相似文献
24.
Bose , Smritimoy , and Jules Janick . (Purdue U., Lafayette, Ind.) Karyo-races in Spinacia oleracea. Amer. Jour. Bot. 48(3): 238–241. Illus. 1961.—Cytological analysis of chromosome 1 of Spinacia oleracea, which contains the XY factor pair, revealed the existence of 3 distinct morphological types. The “standard” chromosome 1, the longest of the complement, is heterobrachial with one arm being about twice as long as the other. The most prevalent situation is for this “standard” chromosome pair to be homomorphic in staminate, monoecious, or pistillate plants. Some staminate and pistillate plants in the varieties ‘Spica’ and ‘Universal’ were found to be heteromorphic for the standard chromosome and a variant type, distinguished by a satellite on the short arm. In a cross derived from an accession PI 169671 from Turkey, which segregated for monoecious and pistillate types, half of the plants, irrespective of sex, were homomorphic for the standard chromosome and half were heteromorphic. The non-standard chromosome was longer and isobrachial, the added length apparently due to an additional segment on the short arm of the “standard” chromosome. Plants homomorphic for the satellited and isobrachial chromosome have been obtained. 相似文献
25.
Summary Six different strains ofProspaltella perniciosi have been tested since 1957 with regard to their efficacy as parasites of the San José scaleQuadraspidiotus perniciosus under the conditions of Western and Southern Switzerland. A Russian, a Chinese and a North-American strain from Illinois
gave the best results both in laboratory rearing and when applied in experimental orchards. The Illinois strain achieved 20%
parasitization already within the first year after its release. Both the Chinese and the Russian strains reached 40% of parasitization
during the second year, and more than 80% during the third year. This was followed by a complete breakdown of the San-José
scale population. These results were promising enough to justify tests on a larger scale in whichP. perniciosi is being released systematically on greater surfaces. The parasite can, apparently, survive even when the host population
is small.
相似文献
26.
BACKGROUND: The gorgeous frescoes organized by the master Renaissance painter Raphael Sanzio (1483-1520) and illustrating the heavenly adventures of Cupid and Psyche were painted between 1515 and 1518 to decorate the Roman villa (now known as the Villa Farnesina) of the wealthy Sienese banker Agostino Chigi (1466-1520). Surrounding these paintings are festoons of fruits, vegetables and flowers painted by Giovanni Martini da Udine (1487-1564), which include over 170 species of plants. A deconstruction and collation of the cucurbit images in the festoons makes it possible to evaluate the genetic diversity of cucurbits in Renaissance Italy 500 years ago. FINDINGS: The festoons contain six species of Old World cucurbits, Citrullus lanatus (watermelon), Cucumis melo (melon), Cucumis sativus (cucumber), Ecballium elaterium (squirting cucumber), Lagenaria siceraria (bottle gourd) and Momordica balsamina (balsam apple), and two or three species of New World cucurbits, Cucurbita maxima, C. pepo and, perhaps, C. moschata (pumpkin, squash, gourd). The images of C. maxima are the first illustrations of this species in Europe. 相似文献
27.
Hepatoproliferin (HPF), a liver regeneration factor that was able to augment the growth of hepatocytes in the presence of EGF, was produced by young rat livers and hepatectomized adult livers (70%), but not by adult intact livers. Therefore only growing and regenerating livers produce HPF. This growth factor was purified into two homogeneous bioactive species having different single SDS-PAGE bands at 18.5 and 14 kDa, and different single pI-bands at pH 4.3 and 8.7, respectively. HPF was synthesized de novo by hepatocytes in the liver as shown by the in vivo incorporation of radiolabeled 35S-sulfate and 14C/3H-glucosamine. This radioactive HPF was secreted ex vivo by hepatocytes, probably to act as an autocrinal hepatomitogen since 90% was found in the growth medium. HPF was neither a classical peptido-mitogen nor a heparin binding growth factor, but a liver-originated non-proteinaceous factor, which probably contains sulfonated saccharides such as glucosamine sulfate. HPF was neither a polyglycan nor a glycopeptide nor a peptidoglycan. 相似文献
28.
Thijs G Moreau Y De Smet F Mathys J Lescot M Rombauts S Rouze P De Moor B Marchal K 《Bioinformatics (Oxford, England)》2002,18(2):331-332
INCLUSive allows automatic multistep analysis of microarray data (clustering and motif finding). The clustering algorithm (adaptive quality-based clustering) groups together genes with highly similar expression profiles. The upstream sequences of the genes belonging to a cluster are automatically retrieved from GenBank and can be fed directly into Motif Sampler, a Gibbs sampling algorithm that retrieves statistically over-represented motifs in sets of sequences, in this case upstream regions of co-expressed genes. 相似文献
29.
Said Ahmaidi Janick Masse-Biron Brigitte Adam Dominique Choquet Michel Freville Jean-Pierre Libert Christian Prefaut 《European journal of applied physiology and occupational physiology》1998,78(2):170-176
This study assessed clinical and cardiorespiratory responses after an interval training programme in sedentary elderly adults
using the ventilatory threshold (V
th) as the index of exercise training intensity. A selection of 22 subjects were randomized into two groups: 11 subjects served
as the training group (TG) and the others as controls (CG). Maximal exercise tests were performed on a treadmill before (T0), each month (T1, T2) and after the 3-month interval training programme period (T3). The TG subjects were individually trained at the heart rate corresponding to V
th measured at T0, T1 and T2 as the breakpoint in the oxygen uptake-carbon dioxide production relationship. Their training programme consisted of walking/jogging
sessions on a running track twice a week. The sessions consisted of varying durations of exercise alternating with active
recovery in such a way that the subjects slowly increased their total exercise time from an initial duration of 30 min to
a final duration of 1 h. During training the heart rate was continuously monitored by a cardiofrequency meter. Compared with
the daily activities of the controls, no training programme-related injuries were observed in TG. Moreover, programme adherence
(73%) and attendance (97.3%) were high. The maximal oxygen uptake and V
th were increased in TG, by 20% (P<0.05) and 26% (P<0.01), respectively. Interval training at V
th also significantly increased maximal O2 pulse (P<0.05) and maximal ventilation (P<0.01). A significant decrease in submaximal ventilation (P<0.05) and heart rate (P<0.01) was also noted. These results would suggest that for untrained elderly adults, an interval training programme at the
intensity of V
th may be well-tolerated clinically and may significantly improve both maximal aerobic power and submaximal exercise tolerance.
Accepted: 6 January 1998 相似文献
30.
Cleistogamy in Salpiglossis sinuata L. (Solanaceae) involves a series of developmental events that includes germination of pollen tetrads en masse inside the anther, penetration of pollen tubes through the anther wall toward the stigma which is in contact with the anther, growth of pollen tubes down the style, and, finally, fertilization. This occurs within 6 days from the first appearance of the flower as compared to 10–12 days for normal anthesis of chasmogamous flowers. Coincident with these events in cleistogamous flowers are lack of corolla development and reduced size of androecium and gynoecium. 相似文献