全文获取类型
收费全文 | 927篇 |
免费 | 62篇 |
出版年
2024年 | 3篇 |
2023年 | 6篇 |
2022年 | 18篇 |
2021年 | 44篇 |
2020年 | 31篇 |
2019年 | 35篇 |
2018年 | 41篇 |
2017年 | 25篇 |
2016年 | 37篇 |
2015年 | 57篇 |
2014年 | 64篇 |
2013年 | 66篇 |
2012年 | 73篇 |
2011年 | 69篇 |
2010年 | 41篇 |
2009年 | 33篇 |
2008年 | 42篇 |
2007年 | 55篇 |
2006年 | 38篇 |
2005年 | 46篇 |
2004年 | 42篇 |
2003年 | 25篇 |
2002年 | 21篇 |
2001年 | 3篇 |
2000年 | 8篇 |
1999年 | 6篇 |
1998年 | 9篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1970年 | 1篇 |
排序方式: 共有989条查询结果,搜索用时 15 毫秒
131.
Bruna M. Schweinberger André F. Rodrigues Elias Turcatel Paula Pierozan Leticia F. Pettenuzzo Mateus Grings Giselli Scaini Mariana M. Parisi Guilhian Leipnitz Emilio L. Streck Florencia M. Barbé-Tuana Angela T. S. Wyse 《Molecular neurobiology》2018,55(2):980-988
In the current study, we verified the effects of maternal hypermethioninemia on the number of neurons, apoptosis, nerve growth factor, and brain-derived neurotrophic factor levels, energy metabolism parameters (succinate dehydrogenase, complex II, and cytochrome c oxidase), expression and immunocontent of Na+,K+-ATPase, edema formation, inflammatory markers (tumor necrosis factor-alpha and interleukin-6), and mitochondrial hydrogen peroxide levels in the encephalon from the offspring. Pregnant Wistar rats were divided into two groups: the first one received saline (control) and the second group received 2.68 μmol methionine/g body weight by subcutaneous injections twice a day during gestation (approximately 21 days). After parturition, pups were killed at the 21st day of life for removal of encephalon. Neuronal staining (anti-NeuN) revealed a reduction in number of neurons, which was associated to decreased nerve growth factor and brain-derived neurotrophic factor levels. Maternal hypermethioninemia also reduced succinate dehydrogenase and complex II activities and increased expression and immunocontent of Na+,K+-ATPase alpha subunits. These results indicate that maternal hypermethioninemia may be a predisposing factor for damage to the brain during the intrauterine life. 相似文献
132.
Mateus Eugenio Loraine Campanati Nathalia Müller Luciana F. Romão Jorge de Souza Soniza Alves-Leon Wanderley de Souza Celso Sant’Anna 《Cytotechnology》2018,70(6):1607-1618
Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of biogenically produced Ag/AgCl-NPs (from yeast cultures) on the proliferation of GBM02 glioblastoma cells (and of human astrocytes) by automated, image-based high-content analysis (HCA). We compared the effect of 0.1–5.0 µg mL?1 Ag/AgCl-NPs with that of 9.7–48.5 µg mL?1 temozolomide (TMZ, chemotherapy drug currently used to treat glioblastomas), alone or in combination. At higher concentrations, Ag/AgCl-NPs inhibited GBM02 proliferation more effectively than TMZ (up to 82 and 62% inhibition, respectively), while the opposite occurred at lower concentrations (up to 23 and 53% inhibition, for Ag/AgCl-NPs and TMZ, respectively). The combined treatment (Ag/AgCl-NPs?+?TMZ) inhibited GBM02 proliferation by 54–83%. Ag/AgCl-NPs had a reduced effect on astrocyte proliferation compared with TMZ, and Ag/AgCl-NPs?+?TMZ inhibited astrocyte proliferation by 5–42%. The growth rate and population doubling time analyses confirmed that treatment with Ag/AgCl-NPs was more effective against GBM02 cells than TMZ (~?67-fold), and less aggressive to astrocytes, while Ag/AgCl-NP?+?TMZ treatment was no more effective against GBM02 cells than Ag/AgCl-NPs monotherapy. Taken together, our data indicate that 2.5 µg mL?1 Ag/AgCl-NPs represents the safest dose tested here, which affects GBM02 proliferation, with limited effect on astrocytes. Our findings show that HCA is a useful approach to evaluate the antiproliferative effect of nanoparticles against tumor cells. 相似文献
133.
Carlos?A.?Salvador-MontoyaEmail author Orlando?F.?Popoff Mateus?Reck Elisandro?R.?Drechsler-Santos 《Plant Systematics and Evolution》2018,304(3):445-459
Morphological revision of Fulvifomes robiniae, as well as phylogenetic inferences based on nITS and nLSU markers, indicated that the species has a narrower concept in its morphology and distribution. Other morphologically related taxa arise from this taxonomic approach. Fulvifomes cedrelae is not accepted as a synonym of F. robiniae, and Fulvifomes squamosus sp. nov. is described as new based on Peruvian specimens. Based on morphology, phylogenetic relationships and host distributions, the taxonomic implication for the genus and other related taxa are discussed. 相似文献
134.
Patricio H. Manríquez Ricardo Guiñez Alberto Olivares Marcela Clarke Juan Carlos Castilla 《Marine Biology Research》2018,14(5):462-477
Reproduction in tunicates is considered to be particularly vulnerable to changes in seawater temperature. In the present study we investigated the effects of sea surface temperature and temperature anomalies on reproductive traits of the non-native sessile tunicate Pyura praeputialis. Reproductive traits of this species were investigated over the course of 67 months, based on samples collected at two localities (eastern and western shorelines) of the Bay of Antofagasta. The study period included years with different oceanic and atmospheric conditions: a warm event, El Niño (June 1997 to July 1998); a cold event, La Niña (August 1998 to December 2000); and post-La Niña (January 2000 to December 2002). We compared two common indices (condition and gonadosomatic) and histological sections to evaluate the maximum reproduction index of this introduced species that dominates a large part of the rocky intertidal habitat in the Bay of Antofagasta. We found sexually mature individuals all year-round and a decrease in both reproductive indices matching the reduction in the sea surface temperature during the austral autumn and winter months. The results suggest that gonad development in this species is sensitive to sea surface temperature and thermal anomalies. We conclude that future thermal anomalies or projected global average sea surface warming associated with climate change might have no negative consequences on these reproductive traits of P. praeputialis. This suggests this species is ecologically resilient and that the ecosystem services that this species provides for other invertebrate and algal species will be maintained. 相似文献
135.
Sebastian A. Klarian Carlos Canales-Cerro Patricio Barría Patricia Zárate Francisco Concha Sebastián Hernández 《Marine Biology Research》2018,14(2):173-182
The blue shark (Prionace glauca) and the shortfin mako shark (Isurus oxyrinchus) are two large and highly migratory sharks distributed in most oceans. Although they are often caught in the south Pacific Ocean long-line fisheries, their trophic ecology is poorly understood. Stable isotopes with Bayesian mixing and dependence concentration models were performed to determine the diet and trophic differences between the two species in the South-eastern Pacific Ocean. According to the mixing models, fishes are the most important prey of these sharks. Dolphin calves and remains were found in the stomachs of both species, which represents a novel finding in trophic ecology of South Pacific sharks. Intra-specific differences were found in P. glauca, but not in specimens of I. oxyrinchus. The two sharks showed a high degree of diet overlap (73%), primarily over mackerel and dolphin carcasses. Our results indicate that blue and shortfin mako sharks have a generalist feeding strategy in the eastern Pacific Ocean, with a strong preference for teleost fishes and also for dolphin carcasses. Therefore, trophic studies are useful to understand energy flow through the food web, and the trophic position of key species. 相似文献
136.
Top predator fish assemblages in Northern Patagonia,Argentina. What factors regulate their patterns of distribution and abundance? 下载免费PDF全文
María Valeria Fernández Mailén Lallement Magalí Rechencq Pablo H. Vigliano Alejandro Sosnovsky Patricio J. Macchi 《Austral ecology》2018,43(6):651-662
According to assemblage theory, three factors regulate fish biogeography: restriction of dispersion, environmental restrictions and biotic interactions. The first two factors act on a regional scale and delimit the area of action of the third, which operates on a local scale. Salmonid introductions began in Patagonia in 1904, and this has led to a restructuring of trophic webs and an increase in the number of top predators. This situation allowed us to evaluate, in a natural setting, how communities are formed on different geographic scales. We studied two large basins in Patagonia, situated close to each other but with different assemblages of top predatory fish. We hypothesized that differences in the structuring of the top predator assemblages between and within the basins are due to 1) environmental factors and dispersion processes facilitated by connectivity on a regional scale; and 2) biotic interaction (internal dynamics) between native perch and salmonids, the former acting as a modulator of the top predator assemblages on a local scale. To test these hypotheses, we analysed the top predator assemblages of 16 lakes and one reservoir, as well as their environmental characteristics. We performed a cluster analysis and related the resulting assembly groups to environmental factors by means of a tree model. We also analysed fish diets, using a similarities test to study biotic interactions. On regional and local scales, water basin, degree of connectivity, area, temperature and Zoogeographic integrity coefficient (ZIC) were important factors in the structuring of top predator assemblages. On a local scale, creole perch modulates the salmonid populations through feeding and the consequent distribution of resources. Our work showed that the structure of top predator assemblages was determined by a combination of local and regional factors acting in synergy, as postulated by the assemblage theory. 相似文献
137.
The age and growth of the pintado Pseudoplatystoma corruscans were studied during the period from May 1994 to May 1995. The standard length ranged from 52 to 145 cm and the weight from 1.3 to 41 kg. The biometric relationship between the standard length (Ls) and total length (Ltotal) and between the total weight (Wt) and the standard length (Ls) were obtained for the species, being respectively: Ltotal = 3.296 + 1.069 * Ls and Wt = 0.00624 * Ls(3.134). The condition factor calculated monthly suggests the spawning season to be between the months of February and March. The age was estimated by counting growth rings present in the spines of the pectoral fins, and 10 age classes were detected. The mean distance of the last ring until the border of the spine suggests that the period of least growth is between July and September (dry period). Von Bertalaffy's equation describing the growth of the pintado is: Lt= 183 * [1 - exp - 0.085 * (t + 3.274)]. Total mortality was Z = 0.24 year(-1) and natural mortality M = 0.20 year(-1). As the present level of exploitation, F = Z - M = 0.04 year(-1), we conclude that the pintado stock was still underexploited in the Pantanal in the sampled period. 相似文献
138.
Genetic diversity and geographical dispersal in grapevine clones revealed by microsatellite markers.
Intravarietal genetic diversification associated with geographical dispersal of a vegetatively propagated species was studied using grapevine Vitis vinifera L. 'Cabernet Sauvignon' as a model. Fifty-nine clonal samples obtained from 7 countries (France, Chile, Spain, Australia, Hungary, USA, and Italy) were analyzed using 84 microsatellite markers. Eighteen polymorphic microsatellite loci (21.4%) were detected, finding 22 different genotypes in the population analyzed with a genetic similarity of over 97%. The presence of chimeric clones was evidenced at locus VMC5g7 by means of a segregation analysis of descendants by self-pollination of a triallelic Chilean clone and by somatic embryogenesis analysis, showing a mutation in L2 cell layer. Only 2 clones (obtained from France and Australia) presented the ancestral genotype, and the most divergent genotype was exhibited by another French clone, which had accumulated 5 somatic mutations. The 2 largest populations considered (from France and Chile) showed a clear divergency in the polymorphisms detected. These antecedents enabled the tracing of geographical dispersal with a phylogenetic hypothesis supporting France as the center of origin of diversification of Cabernet Sauvignon. The results obtained could help to explain diversification processes in other grapevine cultivars. The possibility that this kind of genetic variability occurs in other vegetatively propagated species is discussed, focusing on possible fingerprinting applications. 相似文献
139.
Trophic polymorphism, habitat and diet segregation in Percichthys trucha (Pisces: Percichthyidae) in the Andes 总被引:1,自引:0,他引:1
Daniel E. Ruzzante Sandra J. Walde Victor E. Cussac Patricio J. Macchi Marcelo F. Alonso 《Biological journal of the Linnean Society. Linnean Society of London》1998,65(2):191-214
Divergent natural selection affecting specific trait combinations that lead to greater efficiency in resource exploitation is believed to be a major mechanism leading to trophic polymorphism and adaptive radiation. We present evidence of trophic polymorphism involving two benthic morphs within Percichthys trucha , a fish endemic to temperate South America. In a series of lakes located in the southern Andes, we found two morphs of P. trucha that could be distinguished on the basis of gill raker length and five other morphological measures, most of which are likely associated with the use of food resources. The differences were consistent across all lakes examined, and were correlated with habitat use and diet. Individuals with longer gill rakers were more abundant in the littoral zone (littoral morph) while the short gill-raker morph was more abundant at 10 m depth and deeper (deep benthic morph). Both morphs fed primarily on benthic invertebrates, but the littoral morph fed more on larval Anisoptera than did the deep benthic morph. Phenotypic correlations among traits were high for the littoral morph, but low and non-significant for the deep-benthic morph. We suggest that gill raker length may influence the relative efficiency of suction feeding for the two morphs. This is the first evidence of trophic polymorphism in fishes from temperate South America. 相似文献
140.
Ana L. Obaid Alcides F. Rega Patricio J. Garrahan 《The Journal of membrane biology》1972,9(1):385-401
Summary Maleic anhydride (MA) has been shown to react specifically and rapidly with amino groups of proteins; the maleyl amino groups are negatively charged and completely stable at neutral pH. Treatment of human red cells with this reagent results in a significant increase in K+ permeability which is associated with a much smaller increase in Na+ permeability. Opposite effects are observed on anion permeability, the SO
4
––
and Cl– permeability being decreased to an approximately similar extent upon treatment with MA.Studies on the distribution of MA between membrane lipids and proteins shows that most of the membrane-bound MA is associated with membrane proteins. These results suggest that the observed effects of MA on the ion permeability of the red cell are caused by its combination with amino groups of cell membrane proteins.Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina (CONICET).Established Investigators of CONICET. 相似文献