Versuche über die Regeneration des Abdominalendes von Tenebrio molitor wÄhrend seiner postembryonalen Entwicklung

Ohne Zusammenfassung     

MZmine 2: Modular framework for processing,visualizing, and analyzing mass spectrometry-based molecular profile data

Background  

Mass spectrometry (MS) coupled with online separation methods is commonly applied for differential and quantitative profiling of biological samples in metabolomic as well as proteomic research. Such approaches are used for systems biology, functional genomics, and biomarker discovery, among others. An ongoing challenge of these molecular profiling approaches, however, is the development of better data processing methods. Here we introduce a new generation of a popular open-source data processing toolbox, MZmine 2.     

Steppe-like grassland as a refuge of the wild edible and medicinal plant species in anthropogenic landscape in northeastern Croatia

Habitat fragmentation and modification have been recognized as the most serious threats to biodiversity. In Croatia, the intensive human activities resulted in the landscape transformation, reduction of grassland areas and loss of species number. Increasingly threatened are the wild edible and medicinal plants which contribute remarkably to the biodiversity of different grassland types. In northeastern Croatia, one of the last remnants of the steppe-like grassland remained preserved in a very complex anthropogenic landscape. We examined the diversity of vascular plants occurring on this small habitat patch. Among the relatively high number of plant taxa (in a total of 177) found during the two years of investigations, most could be used both for human nutrition (28 taxa), and for the treatment of a wide range of diseases (60 taxa), while some taxa have nutritional value for livestock (17 taxa). All 23 recorded taxa with edible and medicinal properties are threatened in Croatia, 21 are threatened across Europe, and two are Red Listed taxa. Our results showed that small and isolated steppe-like grassland represents an important refuge for variety of valuable plant species in intensively used landscapes. Accordingly, such small habitats should be, at least locally, effectively protected.     

Changes in nonlinear characteristics of heart rate variability under functional load on the cardiovascular system in healthy subjects and coronary heart disease patients

Some nonlinear characteristics of heart rate variability in the course of functional tests with physical exercise are described. Two groups of volunteers participated in the tests: a control group of 32 healthy subjects (group 1) and a group of 35 coronary heart disease (CHD) patients (group 2). Two series of experiments were performed for each group. An active orthostatic test (AOT) was used in the first series, and a gradually growing physical load on a bicycle ergometer (bicycle ergometer test, BET), in the second series. Along with statistical indices of heart rate (the mean RR interval and standard deviation), nonlinear indices of heart rate were estimated: the correlation dimensionality (D ₂) and approximate entropy (ApEn). Trends of the changes in nonlinear indices of heart rate have been found. The D ₂ and ApEn decreased in both groups of subjects during the AOT and BET under the maximum load. However, the groups of healthy subjects and CHD patients differed in the reactivity of indices, the amplitude of changes in nonlinear indices being narrower in the latter group than in group 1. Differently directed shifts in standard deviation (SDNN) and nonlinear indices have been found. Thus, the data obtained with the use of nonlinear heart rate characteristics show that heart rate under physical load is more multivariate and diverse in healthy subjects at rest and the amplitude of changes during the AOT and BET is greater than in CHD patients, which is a result of the specific autonomic control of heart activity in cardiovascular pathologies.     

Effects of high metal concentrations on antioxidative system in Phragmites australis grown in mine and flotation tailings ponds

Plant and Soil - Phragmites australis grows as a pioneer plant species in several mine and flotation tailings ponds distinguished by extremely high concentrations of metals. The main goals of this...     

Über Restitutionserscheinungen an Stelle von Augen bei Tenebrio-Larven nach Zerstörung der optischen Ganglien

Ohne Zusammenfassung     

Hematopoietic stem cell and mesenchymal stem cell population size in bone marrow samples depends on patient’s age and harvesting technique

Mesenchymal stem cells (MSCs) are heterogeneous population of cells with great potential for regenerative medicine. MSCs are relatively easy to expand in a cell culture, however determination of their concentration in harvested tissue is more complex and is not implemented as routine procedure. To identify MSCs collected from bone marrow we have used two combinations of cell markers (CD45^?/CD73⁺/CD90⁺/CD105⁺ and CD45^?/CD271⁺) and fibroblast colony-forming unit (CFU-F) assay. Further, in donors of various ages, mesenchymal stem cell concentration was compared with the result of CFU-F assay and with hematopoietic stem cell concentration, determined by a standardized flow cytometric assay. A positive correlation of MSC populations to the CFU-F numbers is observed, the population of the CD45^?/CD271⁺ cells correlates better with CFU-F numbers than the population of the CD45^?/CD73⁺/CD90⁺/CD105⁺ cells. The relationship between the hematopoietic CD45^dim/CD34⁺ cell concentration and mesenchymal CFU-Fs or CD45^?/CD271⁺ cells shows a positive linear regression. An age-related quantitative reduction of hematopoietic CD45^dim/CD34⁺, mesenchymal CD45^?/CD73⁺/CD90⁺/CD105⁺ and CD45^?/CD271⁺ stem cells, and CFU-F numbers were noted. Additionally, statistically significant higher CFU-F numbers were observed when bone marrow samples were harvested from three different sites from the anterior iliac crest instead of harvesting the same sample amount only from one site.