List of publications on human biometeorology in Czechoslovakia (period 1920–1960)

International Journal of Biometeorology -     

Ultrastructure of the surface of multiple scars inSaccharomycodes ludwigii

The use of induced primuline fluorescence led to the discovery of a new type of yeast scars (multiple scars) in the generaKloeckera, Saccharomycodes, Nadsonia andHanseniaspora. The structure and ultrastructure of their surface was studied by electron microscopy, using carbon replicas and isolated cell walls.     

Ribosomes in growing and non-growing bacterial cells

1. 1.
   It was found that growing cells always contained more different groups of ribosomes and their total amount was greater than in non-growing cells or spores, in which only two ribosome groups with different sedimentation constants, according to the species of microorganism, were consistently found.     

Contribution to the structural characterization of gamma globulin from pig colostrum

From the results obtained in the present work it is concluded that gamma globulin of the 7 S type (γG) which represents the main immunoglobulin component of pig colostrum, differs from serum γG globulin by the presence of another type of polypeptide chain, designed L₂; the latter was detected after S-sulfonation in starch gel electrophoresis as the fastest moving component and its immunoelectrophoretic pattern shows the presence of two precipitating components. Preparation of soluble heavy and light chains enabled us to study them imunochemically. The heavy chain is represented by two zones; the fainter one was not detected in comparative analysis of the heavy chain of serum γG globulin. The L₁ chain of colostral gamma globulin and the light chain of serum gamma globulin seem to contain three precipitating components, one of which appears due to aging of the chain solution in the presence of glycine. The material from colostrum seems to contain a larger amount of this component than serum. Further it was shown that the component arising in this way is antigenically closely related to the heavy chain.     

Study of the growth and development ofChlorella populations in the culture as a whole

Colour tests for study of the nuclear apparatus ofChlorella cells are described. It can be concluded from the results thatChlorella cell nucleus contains a relatively large nucleolus; the peripheral layers of the nucleus are rich in chromatin only in the nuclear division phase. The nucleus divides by a simplified form of mitosis, characterized morphologically by simple constriction.     

Preparation of anti-inhibitor serum and its effect on the multiplication of influenza virus

Antiserum был подготовлен противингибитор вируса haemagglutination (IVH)одна из эмбриона chorioallantoic мембр анныепутем иммун изации морских св инокЭто анти-инги битора в сыворотке заблокирован ингибирование IVH из chorioallantoicмембран в haemagglutination испытанияно ника кого влияния на ре цепторы эритроци товСыворотке кро] ви не влияет рост chorioallantoic мембраны клет ок инет нейтрализ овать воздействи е на вирус гриппа Она снизилась спо собность chorioallantoic мембр ан для adsorb вируса гри ппа, а также степен ь размножения вир усав такого рода тканиАвторы хоте ли бы поблагодари ть г-н Г. Ruttkay-Ne -палубе и для проведения эле ктрофореза меру ния.     

Three fluorescent probes for the flow-cytometric assessment of membrane potential inSaccharomyces cerevisiœ

Three fluorescent probes, tetramethyl rhodamine ethyl ester (TMRE), 3,3′-dipropylthiacarbocyanine iodide (diS-C₃(3)) and 3,3′-dipropyloxacarbocyanine iodide (diO-C₃(3)), were tested for their suitability as fluorescent indicators of membrane potential inSaccharomyces cerevisiœ in studies performed by flow cytometry. For all these dyes the intensity of fluorescence of stained cells increased with probe concentration in the range of 60–3000 nmol/L. The optimum staining period was 15–20 min for diS-C₃(3). Depolarization of cells by increased extracellular potassium level and by valinomycin elicited with all probes a drop in fluorescence intensity. In some yeast batches this depolarization was accompanied by a separation of subpopulations with different fluorescence properties.