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Maryam Ebrahim-Ghomi Bagher Mahmoodi Farshad Rakhshanderoo Masoud Naderpour Peyman Norouzi 《Archives Of Phytopathology And Plant Protection》2016,49(1-4):1-10
Beet necrotic yellow vein virus (BNYVV) is the most devastating pathogen of sugar beet worldwide. This virus has been reported in the majority of sugar beet growing regions of Iran as well. For the present study, we collected samples from different sugar beet varieties with suspected symptoms of BNYVV from the main important sugar beet growing regions in eight provinces of Iran. Infection of collected samples to BNYVV was tested by ELISA and RT-PCR. Upon testing of 167 collected samples of BNYVV suspected through ELISA and RT-PCR, 115 (68.9%) were infected. Different incidences of BNYVV through surveyed provinces may represent the presence of diverse infective viral sources or resistance genes in tested sugar beet varieties which need further attempts to develop control strategies. Results also showed that BNYVV has been recently distributed throughout some surveyed regions. Otherwise, trace infection or resistance to BNYVV infection in some varieties of distinct regions may represent proper sources of resistance to BNYVV. 相似文献
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In north Khorasan province, Iran, we compared the effectiveness of two types of traps for collecting reptiles: funnel traps and pitfall traps. Three stations were monitored over three 10-day periods and in total 544 individuals (including 200 recaptures) belonging to 5 species and 4 families of lizards (Lacertidae) were collected. Funnel traps with 280 captures were more efficient than pitfall traps with 264 captures, but the differences between the two traps are not significant. Three species were captured most often in the three different stations respectively: station 1, Bunopus crassicauda (22% relative frequency); station 2, Eremias fasciata (29% relative frequency); and station 3, Trapelus agilis (32% relative frequency). Shannon species richness indices were higher for pitfall than funnel traps (i.e., 1.45 vs. 1.40), but the difference was not significant. Pitfall traps were better for capturing species that search widely for food, while the funnel traps preferentially captured species that climb plants, such as Trapelus agilis. We recommend using both types of trap to capture the various types of species in any one region. 相似文献
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Fariba Karimzadeh Sayed Mostafa Modarres Mousavi Tahereh Ghadiri Maryam Jafarian Mansoureh Soleimani Shahin Mohammad Sadeghi Masoud Mesgari Mohammad-Taghi Joghataei Ali Gorji 《Molecular neurobiology》2017,54(2):846-854
Modulatory function of metabotropic glutamate type 1 (mGlu1) receptors plays a crucial role in the pathophysiology of some neurological disorders, including schizophrenia and epilepsy. In this study, the expression of mGlu1α receptors in the thalamic nuclei was assessed during development of absence seizures in the WAG/Rij rats, a valid genetic animal model of absence epilepsy. In addition, the effect of pharmacological modulation of mGlu1α receptors in the laterodorsal (LD) nucleus of the thalamus on the characteristic features of bioelectrical brain activities in the WAG/Rij rats was assessed. The expression of mGlu1α receptors in the LD was assessed in four experimental groups of both WAG/Rij and Wistar rats with 2 and 6 months of age. Agonist and antagonist of mGlu1α receptors were infused in LD in the six months old WAG/Rij (epileptic) rats. The protein level of mGlu1α receptors in the thalamus of the 6-month-old WAG/Rij rats was lower than non-epileptic animals. In addition, the distribution of mGlu1α receptors in different thalamic nuclei was lower in the 6-month-old WAG/Rij compared to age-matched Wistar rats. The gene expression of mGlu1α receptor was also significantly lower in 6-month-old WAG/Rij rats in the LD compared to other animal groups. The microinjection of mGlu1α receptors agonist and antagonist in the LD reduced the duration of spike-wave discharges (SWDs) and increased the amplitude and duration of SWDs, respectively, in 6-month-old WAG/Rij rats. The alterations of mGlu1α receptors expression in the thalamus of epileptic WAG/Rij rats as well as its modulatory effects in the generation of SWDs suggest the potential of mGlu1 receptors as a therapeutic target in absence epilepsy. 相似文献
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Fereshteh Azedi Somaieh Kazemnejad Amir Hassan Zarnani Masoud Soleimani Amir Shojaei Shaghayegh Arasteh 《Molecular biology reports》2017,44(1):169-182
In order to characterize the potency of menstrual blood stem cells (MenSCs) for future cell therapy of neurological disorders instead of bone marrow stem cells (BMSCs) as a well-known and conventional source of adult stem cells, we examined the in vitro differentiation potential of these stem cells into neural-like cells. The differentiation potential of MenSCs to neural cells in comparison with BMSCs was assessed under two step neural differentiation including conversion to neurosphere-like cells and final differentiation. The expression levels of Nestin, Microtubule-associated protein 2, gamma-aminobutyric acid type B receptor subunit 1 and 2, and Tubulin, beta 3 class III mRNA and/or protein were up-regulated during development of MenSCs into neurosphere-like cells (NSCs) and neural-like cells. The up-regulation level of these markers in differentiated neural-like cells from MenSCs was comparable with differentiated cells from BMSCs. Moreover, both differentiated MenSCs and BMSCs expressed high levels of potassium, calcium and sodium channel genes developing functional channels with electrophysiological recording. For the first time, we demonstrated that MenSCs are a unique cell population with differentiation ability into neural-like cells comparable to BMSCs. In addition, we have introduced an approach to generate NSCs from MenSCs and BMSCs and their further differentiation into neural-like cells in vitro. Our results hold a promise to future stem cell therapy of neurological disorders using NSCs derived from menstrual blood, an accessible source in every woman. 相似文献
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Afsaneh Salahvarzi Mohamad Mahani Masoud Torkzadeh-Mahani Reza Alizadeh 《Analytical biochemistry》2017
An immunoassay method based on the peak shift of the localized surface plasmon resonance (LSPR) absorption maxima has been developed for the determination of the thyroid stimulating hormone (TSH) in human blood serum. The anti-TSH antibody was adsorbed on the synthesized gold nanoparticles by electrostatic forces. The efficiency of the nanobiosensor was improved by optimizing the factors affecting the probe construction such as the pH and the antibody to gold nanoparticles ratio. Dynamic light scattering was applied for the characterization of the constructed probe. The amount of peak shift of the LSPR absorption maxima was selected as the basis for determination of TSH antigen. The linear dynamic range of 0.4–12.5 mIU L−1 and the calibration sensitivity of 1.71 L mIU−1 were obtained. The human control serum sample was analyzed for TSH by constructed nanobiosensor and the acceptable results were obtained. 相似文献
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