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991.
Tsutomu Nakanishi Kyoko Iwasaki Masao Nasu Iwao Miura Kaisuke Yoneda 《Phytochemistry》1982,21(7):1759-1762
Aristoloside, a new companion aristolochic acid derivative isolated from stems of Aristolochia manshuriensis has been shown to be 6-O-β-d-glucopyranoside of aristolochic acid-D on chemical and physicochemical evidence. Three known acids, aristolochic acids I, IV (both as their corresponding methyl esters), and -D have also been characterized from stems of the plant. 相似文献
992.
Masao Tanihara Yasushi Kikuchi Yukio Imanishi 《International journal of biological macromolecules》1982,4(5):297-304
Tripeptides with cyclic dipeptide backbones, cyclo[l-Glu(l-Leu-O Bzl)-t-His] and cyclo[l-Glu(l-Leu-OH)-Ir His, and the corresponding tripeptides with linear backbones, Me3COCO-l-Glu(l-Leu-OBzl)-l-His-OMe and Me3COCO-l-Glu(l-Leu-OH)-l-His-OMe, were synthesized and used as catalysts for the hydrolysis of carboxylic acid active esters of various types. The experimental results are summarized as follows. (I) In the hydrolysis of a neutral and hydrophobic substate, p-nitrophenyl laurate, in 20% dioxane/H2O mixture of pH 7.8, a hydrophobic and flexible peptide, Me3COCO-l-Glu(l-Leu-OH)-l-His-OMe, was more reactive than imidazole. On the other hand, cyclo[l-Glu(l-Leu-OBzl)-l-His] and cyclo[l-Leu-OH)-l-His], which have rigid backbone chain and fixed sidechain conformation, were not particularly reactive. (2) in the solcolysis of a positively charged substrate, p-nitrophenyl glycinate hydrochloride, in 42% i-PrOH/H2O mixture at pH 6.95, a positively charged substrate, p-nitrophenyl glycinate hydrochloride, in 42% i-PrOH/H2O mixture at pH 6.95, a negatively charged and flexible peptide, Me3COCO-l-Glu(l-Leu-OH)-l-His-OMe, was more reactive than imidazole. However, cyclo [l-Glu(l-Leu-OH)-l-His] was not particularly reactive in the same reaction. In the hydrolysis of p-nitrophenyl glycinate hydrochloride in aqueous solution at pH 7.8 a hydrophobic and rigid peptide, cyclo[(l-Glu(l-Leu-OBzl)-l-His], was more reactive than imidazole. However, in the hydrolysis of p-nitrophenyl CO-AMINODODECANOATE hydrochloride, which has a positive charge and a rective site separated by a long hydrophobic chain, peptide catalysts did not show efficient catalysis. (3) In the hydrolysis of a positively charged, hydrophobic and chiral substrate, p-nitrophenyl leucinate hydrochloride, in aqueous solution at pH 6.95, the d-enantiomer was hydrolysed more quickly that the t-enantiomer with cyclo[l-Glu(l-Leu-OBzl)l-His] or cyclo[t-Glu(l-Leu-OH)-l-His] as catalyst. On the other hand, the tripeptides with linear backbone did not effect an enantiomer-selective catalysis. The solvolytic reaction catalysed by the tripeptides with cyclic dipeptide backbone in 42% i-PrOH/water mixture was also enantiomer-selective. 相似文献
993.
Yasushi Kikuchi Masao Tanihara Yukio Imanishi 《International journal of biological macromolecules》1982,4(5):305-312
Conformation in aqueous solution at pH 6.95 of tripeptides having cyclic dipeptide backbones, cyclo[l-Glu(l-Leu-OBzl)-l-His] and cyclo[l-Glu(l-Leu-OH)-l-His], was investigated by u.v., c.d. and n.m.r. spectroscopy and by the lanthanide probe method. In the major conformation of cyclo[l-Glu(l-Leu-OBzl)-l-His], the cyclic dipeptide backbone takes a flagpole-boat conformation in which the sidechain of the l-His residue is nearly parallel with the backbone plane and the sidechain of the l-Glu residue protrudes outside the backbone plane. In the major conformation of cyclo[l-Glu(l-Leu-OH)-l-His], the cyclic dipeptide backbone takes a flagpole-boat conformation in which the sidechains of the l-His and l-Glu residues are accommodated in the same side of the backbone plane so that the imidazolyl sidechain of l-His residue is twisted slightly. Tripeptides were not found to change the conformation when metal salts or ammonium salts such as Cl?H3N?(CH2)11 COOEt, Gly-OEt-HCl, dl-Val-OEt-HCl and l-Leu-OEt-HCl were added, but a significant conformation change occurred upon adding d-Leu-OEt·HCl. If the same situation holds with the addition of α-amino acid p-nitrophenyl ester hydrochlorides, the previously reported enantiomer-selective catalysis by the tripeptides which hydrolysed d-Leu-OPh(NO2·HCl faster than l-Leu-OPh(NO2)·HCl can be explained; that is, the tripeptides change the conformation only when d-Leu-OPh(NO2)·HCl is bound and consequently the intramolecular reaction is facilitated. This phenomenon may be compared with that of ‘induced fit’ in enzyme catalysis. 相似文献
994.
Kanji Ishizaki Takashi Yagi Masao Inoue Osamu Nikaido Hiraku Takebe 《Mutation research》1981,80(1):213-219
Sensitivities to UV and mitomycin C (MC) of fibroblasts obtained from 3 Japanese patients with Bloom's syndrome (BS) were studied. One BS strain was more sensitive to UV than normal cells only in colony-forming ability. Other responses to UV, such as unscheduled DNA synthesis, host-cell reactivation and removal of UV-endonuclease-susceptible sites, were normal in all 3 strains. These BS strains were more sensitive to MC than were normal cells. However, the amounts of unscheduled DNA synthesis after treatment with MC in BS cells did not differ from those in normal cells. 相似文献
995.
Yoshiyuki Suzuki Hitoshi Sakuraba Michel Potier Masao Akagi Masao Sakai Hirokuni Beppu 《Human genetics》1981,58(4):387-389
Summary 4-Methylumbelliferyl neuraminidase activity was studied in fibroblasts, leukocytes, and frozen tissues from adult patients with -galactosidase-neuraminidase deficiency and specific clinical manifestations. This enzyme was almost completely deficient in fibroblasts, but the residual activity was relatively high (20% of the control mean) in the leukocytes from the patients. The frozen liver from one patient showed the enzyme activity as high as controls.This enzyme consisted of two components, freeze-labile and freeze-stable, and it was demonstrated that only the labile enzyme was deficient in fibroblasts and leukocytes. The apparently normal activity of neuraminidase in frozen autopsy tissues of a patient may be explained by the loss of the labile component in control tissues after a long-term freezing. The neuraminidase activity was variable in parents and no definite conclusion was drawn on the hereditary nature of the disease. 相似文献
996.
Parvo-like virus, which was designated as “Ina-flacherie virus (Ina-FV),” was isolated from the silkworm, Bombyx mori, and the properties of its DNA were characterized. Purified Ina-FV had a diameter of 22 ± 0.5 nm and a sedimentation coefficient of 102 S. On density gradient separation in CsCl, particles were found at densities of 1.40 and 1.45 g/ml. The DNA content of Ina-FV was 28 ± 2%. The DNA in low-salt buffer possessed properties typical of a single-stranded (ss) molecule. Double-stranded (ds) DNA was extracted under conditions of appropriate high salt and elevated temperature. Electron microscopical examination revealed that the ds DNA was composed of linear molecules with an average length of 1.7 μm and other less well-defined structures. The linear ds molecule had a molecular weight of about 3.4 × 106 determined by electron microscopy (EM) and agarose gel electrophoresis. When the ds DNA was alkali-denatured and examined in an EM, linear ss molecules with approximate length of 1.7 μm were observed, indicating that the linear ds molecule was formed from the annealing of the linear ss molecules of unit length. These data suggest that Ina-FV is closely related to members of the densovirus subgroup. 相似文献
997.
Yoshinori Asakawa Hiroshi Inoue Masao Toyota Tsunematsu Takemoto 《Phytochemistry》1980,19(12):2623-2626
The distribution of ent-2,3-secoaromadendrane-, ent-aromadendrane-, ent-bicyclogermacrane- and ent-maaliane-type sesquiterpenoids in fourteen Plagiochila species is described. These sesquiterpenes are the significant chemosystematic markers of Plagiochila. The intense pungent substance of some Plagiochila species is due to an ent-2,3-secoaromadendrane-type sesquiterpene hemiacetal, plagiochiline A. 相似文献
998.
Yoshinori Asakawa Masao Toyota Masako Kano Tsunematsu Takemoto 《Phytochemistry》1980,19(12):2651-2654
Three new pinguisane-type sesquiterpenes, dehydropinguisanin, dehydropinguisenol and pinguisenal together with the previously known deoxopinguisone, pinguisanin, pinguisanolide and (—)-bicyclogermacrene, have been isolated from the liverwort, Trocholejeunea sandvicensis, and their structures have been established from spectral evidence. 相似文献
999.
Yoshinori Asakawa Masao Toyota Tsunematsu Takemoto Rudiger Mues 《Phytochemistry》1981,20(12):2695-2699
Three liverworts, Trichocolea tomentella, Neotrichocolea bissethii and Trichocoleopsis sacculata belonging to Jungermanniales were chemically investigated. Isoprenyl benzoates are the important chemical markers of Trichocolea tomentella. Neotrichocolea bissethii elaborates sesquiterpenes as the major components. The sacculatane-type diterpenes and pinguisane-type sesquiterpenes are the significant chemosystematic markers of Trichocoleopsis sacculata. These three species are chemically quite different. Trichocoleopsis sacculata is chemically rather close to Porella species. The present chemical results support the recent morphological classification of the above three species proposed by Schuster. Some species of Jungermanniales are chemically identical to those of Metzgeriales and these results also support the phylogenetic classification in which the two orders have been united within the subclass Jungermanniae. 相似文献
1000.
Koichi Kato Shuichi Ikeyama Muneo Takaoki Akio Shino Masao Takeuchi Atsushi Kakinuma 《Cell》1981,24(3):885-895
By indirect immunofluorescence microscopy, an antiserum raised in rabbit against serum thymic factor (FTS) was found to decorate the epithelial cells not only in the thymus, but also in the kidney, uterus, urinary bladder, prostatic glands, stomach, ileum, colon, submaxillary glands, trachea, epidermis and epidermal appendages of mouse. The staining ability was completely absorbed with an FTS-binding immunoabsorbent, and affinity-purified anti-FTS IgG showed the same staining patterns as the original antiserum. The staining profiles resembled those described for tissues stained with antiprekeratin and antikeratin antibodies in both distribution in tissue and localization in the epithelial cells. In primary-cultured cells from mouse kidney medullae, the anti-FTS antibodies decorated the cytoplasmic fiber network. The fibers were wavy, bundled together and branched. They were dense in the perinuclear cytoplasm and spread in the cytoplasm toward the cell periphery. This decoration was resistant to colchicine and cytochalasin B, but sensitive to pretreatment with formaldehyde. The organization and shape of the fiber network were similar to those of the networks of intermediate-sized filaments containing cytokeratins, keratins and vimentin. However, the antiserum did not give a precipitin band in immunodiffusion test with prekeratin from bovine muzzle, keratin from human epidermis or 3T3 vimentin. Neither tubulin nor actin formed precipitin bands with the antiserum. These results show that the epithelial cells of various mouse tissues contain FTS or substances close to FTS in chemical structure and suggest that they are associated with the intermediate-sized filaments. 相似文献