全文获取类型
收费全文 | 700篇 |
免费 | 30篇 |
国内免费 | 1篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 12篇 |
2017年 | 7篇 |
2016年 | 9篇 |
2015年 | 14篇 |
2014年 | 34篇 |
2013年 | 55篇 |
2012年 | 41篇 |
2011年 | 40篇 |
2010年 | 22篇 |
2009年 | 26篇 |
2008年 | 38篇 |
2007年 | 45篇 |
2006年 | 37篇 |
2005年 | 69篇 |
2004年 | 50篇 |
2003年 | 41篇 |
2002年 | 48篇 |
2001年 | 6篇 |
2000年 | 9篇 |
1999年 | 11篇 |
1998年 | 12篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1995年 | 11篇 |
1994年 | 8篇 |
1993年 | 2篇 |
1991年 | 4篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 3篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有731条查询结果,搜索用时 31 毫秒
711.
S Miyabo M Minami T Inazu T Tamai N Aoyagi K Miyanaga E Ooya S Kishida T Nakai 《Hormone research》1989,31(4):163-168
This paper documents the rare and hitherto unreported association between isolated ACTH deficiency and normoreninemic hypoaldosteronism in a 63-year-old woman. Baseline plasma aldosterone and 18-hydroxycorticosterone were extremely low. Both steroids did not respond to exogenous angiotensin II infusion, whereas they were increased in parallel to ACTH stimulation. Thus, acquired dysfunction or congenital dysgenesis of the zona glomerulosa was suspected. The upright posture-furosemide test showed a subnormal but definite plasma aldosterone response coupled with a normal increase in plasma renin activity, indicating that there may be a yet unidentified mechanism(s) underlying the postural increase of aldosterone. 相似文献
712.
713.
Masanobu Miyahara Eiji Okimasu Hiroaki Mikasa Shigeo Terada Hiroyuki Kodama Kozo Utsumi 《Archives of biochemistry and biophysics》1984,233(1):139-150
The mitochondrial dysfunction induced by anoxia in vitro was improved with chlorpromazine, cepharanthine, bromophenacyl bromide, and mepacrine without affecting phospholipid or adenine nucleotide metabolisms. The drugs inhibited lipid peroxidation by Fe2+, mitochondrial disruption by Ca2+, and membrane perturbation by lysolecithin, and retained the activity to control H+ permeability across mitochondrial membranes. The drugs appeared to preserve the functions by acting to suppress the development of membrane deterioration which may have resided in the deenergization of mitochondria in the absence of oxygen. 相似文献
714.
715.
Masanobu Tara 《Journal of plant research》1979,92(2):151-156
In addition to various types of natural hybrids betweenAster ageratoides subsp.ovatus andKalimeris incisa reported earlier, a new backcross type has been discovered. This new type, characterized by the chromosomes of 2n=27L+54S,
was most probably produced through fertilization of a normally-reduced gamete of the F1 plant (2n=72=18L+54S) and an unreduced gamete of subsp.ovatus (2n=36=18L+18S). 相似文献
716.
717.
Masanobu Wada Shuichiro Inashima Takashi Yamada Satoshi Matsunaga 《Journal of applied physiology》2003,94(3):923-929
The effects of endurance training on the expression of myosin were electrophoretically analyzed in the deep portion of vastus lateralis muscle from the rat. A 10-wk running program led to increases (P < 0.01) in myosin heavy chain (MHC) 2a and 2d with a decrease (P < 0.01) in MHC(2b). Training also evoked a rearrangement of the isomyosin pattern with decreases in fast isomyosin (FM) 1 (P < 0.01) and FM2 (P < 0.05) and a rise in intermediate isomyosin (P < 0.01). These changes were accompanied by a 61% decrease (P < 0.01) in myosin light chain (MLC) 3F (11.8 +/- 2.7 vs. 4.6 +/- 4.2%). Two-dimensional electrophoresis made it possible to separate the triplet of isomyosins (FMb) consisting of MHC(2b). Training elicited a 26% decrease (P < 0.05) in the FM1b fraction within FMb, i.e., FM1b/(FM1b + FM2b + FM3b) (24.2 +/- 5.5 vs. 18.0 +/- 4.3%). These changes resulted in a 10% decrease (P < 0.05) in the MLC(3F) fraction, i.e., MLC(3F)/(MLC(1F) + MLC(3F)), in FMb (44.9 +/- 4.5 vs. 40.3 +/- 3.2%). These results suggest that endurance training may exert the depressive effect on the contractile velocity of type IIB fibers and that a training-induced decrease in the contractile velocity of whole muscle may be caused by alterations in fast alkali MLC complements within a given fiber type as well as by transitions in MHC-based fiber populations. 相似文献
718.
Masaki Ogata Takehiko Oomori Hiroyuki Soga Yuta Ota Ari Itoh Takaji Matsutani Masanobu Nanno Ryuji Suzuki Tsunetoshi Itoh 《Cell and tissue research》2009,335(2):371-382
In our earlier work, we found that, in mice, i.p. injection of anti-CD3 monoclonal antibody activated intraepithelial lymphocytes
(iIEL), leading to DNA fragmentation in villous epithelial cells of the duodenum and jejunum within 30 min. By 2 h after injection,
nearly half of the enterocytes had detached from the villi, and DNA fragmentation could barely be detected in the remaining
villous epithelium. We hypothesized that DNA had been repaired in enterocytes in which DNA fragmentation had previously been
induced. In this study, enterocytes became negative for TUNEL staining at 60 min after anti-CD3 treatment, prior to detachment.
The remaining villous epithelial cells, after DNA fragmentation and detachment, were found to be positive for 5-bromo-2-deoxyuridine
labeling. To confirm whether fragmented DNA had been repaired in situ, we investigated the appearance and/or mobilization
of DNA-repair-related proteins. Focus formation, a typical staining pattern of repair-related proteins including phosphorylated
H2AX, phospo-ATM substrate, and Nbs1, was observed 30 min after anti-CD3 injection, with the kinetics virtually identical
to that of DNA fragmentation. The co-localization of γ-H2AX and phospo-ATM substrate was also confirmed. The disappearance
of a positive reaction for TUNEL staining in previously fragmented DNA, the appearance of representative DNA-repair-related
proteins, the coincidence of the kinetics of DNA fragmentation and this appearance of DNA-repair-related proteins, and the
co-localization of two of the repair-related proteins strongly indicated that enterocyte DNA could be repaired after it had
been fragmented in vivo. Thus, DNA fragmentation per se may not necessarily be an immediate sign of cell death.
This work was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture,
Japan (16590132 to T.M., 16390045 to T.I., and 20590181 to M.O.). 相似文献
719.
Takuya Koseki Yozo Miwa Yuichiro Mese Akimasa Miyanaga Shinya Fushinobu Takayoshi Wakagi Hirofumi Shoun Hiroshi Matsuzawa Katsumi Hashizume 《Biochimica et Biophysica Acta (BBA)/General Subjects》2006
A role for N-linked oligosaccharides on the biochemical properties of recombinant α-l-arabinofuranosidase 54 (AkAbf54) defined in glycoside hydrolase family 54 from Aspergillus kawachii expressed in Pichia pastoris was analyzed by site-directed mutagenesis. Two N-linked glycosylation motifs (Asn83–Thr–Thr and Asn202–Ser–Thr) were found in the AkAbf54 sequence. AkAbf54 comprises two domains, a catalytic domain and an arabinose-binding domain classified as carbohydrate-binding module 42. Two N-linked glycosylation sites are located in the catalytic domain. Asn83, Asn202, and the two residues together were replaced with glutamine by site-directed mutagenesis. The biochemical properties and kinetic parameters of the wild-type and mutant enzymes expressed in P. pastoris were examined. The N83Q mutant enzyme had the same catalytic activity and thermostability as the wild-type enzyme. On the other hand, the N202Q and N83Q/N202Q mutant enzymes exhibited a considerable decrease in thermostability compared to the glycosylated wild-type enzyme. The N202Q and N83Q/N202Q mutant enzymes also had slightly less specific activity towards arabinan and debranched arabinan. However, no significant effect on the affinity of the mutant enzymes for the ligands arabinan, debranched arabinan, and wheat and rye arabinoxylans was detected by affinity gel electrophoresis. These observations suggest that the glycosylation at Asn202 may contribute to thermostability and catalysis. 相似文献
720.
Not‐self or denatured nucleic acids are recognized by pattern recognition receptors localized mainly in endosomes and cytoplasm, such as Toll‐like receptor (TLR) 3, TLR7, TLR9, retinoic acid‐inducible gene‐I, DNA‐dependent activator of IFN‐regulatory factors and other receptors. The binding of polyriboinosinic:polyribocytidylic acid (poly I:C), a synthetic dsRNA that robustly induces type I interferon, to a putative cell‐surface receptor on a rabbit kidney cell line, RK13, has been analyzed by the authors and RK13 cells found to capture poly I:C in a specific fashion with sufficient affinity. These findings suggest that an alternative receptor to poly I:C participates in the induction of type 1 interferon, which localizes on cell surfaces. Although the nature of this molecule has not yet been identified, accumulating evidence has led the present authors to speculate that there are undefined classes of RNA‐recognition molecules on cell surfaces and that these are unlikely to be categorized as previously reported dsRNA receptors. Although many years have passed since this possibility was first reported by the present authors, it remains attractive. In this article, previously reported cell‐surface dsRNA receptors are reviewed in comparison with other receptors reported to date that are firmly involved in the innate immune‐sensing of nucleic acids. 相似文献