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901.
Sumiya Ishigami Shinichi Ueno Masataka Matsumoto Hiroshi Okumura Takaaki Arigami Yasuto Uchikado Tetsuro Setoyama Hideo Arima Ken Sasaki Masaki Kitazono Hiroyuki Shinchi Yuko Kijima Shoji Natsugoe 《Cancer immunology, immunotherapy : CII》2010,59(3):389-395
Background and aim
A new marker, CD208, was recently explored as a mature interdigitating dendritic cell (DC), and the correlation between the infiltration of CD208-positive cells and clinical factors has been reported in various types of cancers. In this study, we tried to clarify the clinical implication of CD208-positive cell infiltration in gastric cancer immunohistochemically.Patients and methods
A total of 128 gastric cancer patients who underwent a curative operation were enrolled. DCs in tumor nests were identified with two DC markers, CD208 and S-100 protein (S100), by immunohistochemistry. The correlation between clinicopathological features and the CD208- or S100-positive cell infiltration degree was analyzed.Results
Infiltration of S100-positive cells did not correlate with the degree of CD208-positive cell infiltration. Patients with high CD208-positive cell infiltration in the peritumor had a poorer surgical outcome than those with low CD208 infiltration (p < 0.05). Multivariate analysis revealed that CD208-positive cell infiltration was not an independent prognostic factor.Conclusion
We showed that intratumoral CD208-positive cells, as mature DCs, had an inverse correlation to patients’ postoperative outcome in gastric cancer, unlike a conventional DC marker. Evaluation of CD208-positive cell infiltration with S100-positive cell infiltration in gastric cancer is useful to predict antitumor immunological conditions in gastric cancer. 相似文献902.
903.
Akiko Sakata Masaki Mogi Jun Iwanami Kana Tsukuda Li-Juan Min Fei Jing Masaru Iwai Masaharu Ito Masatsugu Horiuchi 《Life sciences》2010,86(17-18):638-645
AimsSex-specific medicine has been highlighted as a different approach to the diagnosis and treatment of diseases between men and women. Type 2 diabetes has been reported to be a risk factor for cognitive impairment. Here, we investigated the sex difference in cognitive function associated with diabetes using KKAy mice.Main methodsCognitive function was evaluated by shuttle avoidance test and Morris water maze test. Changes in gene expression in the brain were evaluated by PCR array and confirmed by quantitative RT-PCR. To evaluate the effect of estradiol, some female KKAy were ovariectomized and treated with or without estradiol.Key findingsIn KKAy mice, female significantly exhibited impaired cognitive function compared with male, while there was no sex difference in these cognitive functions in C57BL6, wild-type mice. Female KKAy mice showed hyperinsulinemia, impaired glucose tolerance and increased oxidative stress compared with male KKAy mice. Female KKAy also showed a significant decrease in peroxisome proliferators-activated receptor (PPAR)-γ expression in the brain compared with male KKAy. Estradiol treatment improved the insulin resistance and higher superoxide production, but failed to improve the cognitive task performance, serum insulin level and lower expression of PPAR-γ.SignificanceIn diabetic mice, female showed significantly impaired cognitive function, with greater insulin resistance, lower expression of PPAR-γ and higher superoxide production compared with male. Estrogen had little effect on cognitive function. These results indicate that a sex-specific approach to cognitive impairment is necessary for diabetic patients, especially for women. 相似文献
904.
Masaki Takahashi Kentarou Ushijima Yohei Hayashi Tomohiro Maekawa Hitoshi Ando Shu-ichi Tsuruoka Akio Fujimura 《Life sciences》2010,86(1-2):24-29
AimsWhile glucocorticoids are widely used to treat patients with various diseases, they often cause adverse effects such as bone fractures. In this study, we investigated whether the decrease in bone density induced by glucocorticoid therapy was ameliorated by optimizing a dosing-time.Main methodsRats were administered with dexamethasone (Dex) orally (1 mg/kg/day) for 6 weeks at a resting or an active period. After the end of the treatment, bone density of femur, biomarkers of bone formation and resorption, and other biomedical variables were measured.Key findingsBone density of femur was significantly decreased by the 6-week treatment with Dex, and the degree of decrease in the 14 HALO (hours after light on) dosing group (an active period) was larger than that in the 2 HALO dosing group (a resting period). Although urinary calcium excretion was accelerated by Dex treatment, secondary hyperparathyroidism was not detected. Histomorphometry analysis showed that Dex suppressed bone resorption, which was larger in the 2 HALO than in the 14 HALO groups. These data indicate that Dex equally suppressed bone formation in the 2 and 14 HALO groups, but inhibited bone resorption more in the 2 HALO than in the 14 HALO groups.SignificanceThis study shows that the decrease in bone density induced by Dex was changed by its dosing-time. 相似文献
905.
Masaki Igarashi Jun-ichi Osuga Masashi Isshiki Motohiro Sekiya Hiroaki Okazaki Satoru Takase Mikio Takanashi Keisuke Ohta Masayoshi Kumagai Makiko Nishi Toshiro Fujita Ryozo Nagai Takashi Kadowaki Shun Ishibashi 《Journal of lipid research》2010,51(2):274-285
Neutral cholesterol ester hydrolase (NCEH) accounts for a large part of the nCEH activity in macrophage foam cells, a hallmark of atherosclerosis, but its subcellular localization and structure-function relationship are unknown. Here, we determined subcellular localization, glycosylation, and nCEH activity of a series of NCEH mutants expressed in macrophages. NCEH is a single-membrane-spanning type II membrane protein comprising three domains: N-terminal, catalytic, and lipid-binding domains. The N-terminal domain serves as a type II signal anchor sequence to recruit NCEH to the endoplasmic reticulum (ER) with its catalytic domain within the lumen. All of the putative N-linked glycosylation sites (Asn270, Asn367, and Asn389) of NCEH are glycosylated. Glycosylation at Asn270, which is located closest to the catalytic serine motif, is important for the enzymatic activity. Cholesterol loading by incubation with acetyl-LDL does not change the ER localization of NCEH. In conclusion, NCEH is targeted to the ER of macrophages, where it hydrolyzes CE to deliver cholesterol for efflux out of the cells. 相似文献
906.
A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one week is required for the widely used culture-dependent approach. 相似文献
907.
908.
909.
Leptodora kindtii is a major predator of small zooplankton in eutrophic and fish-abundant lakes. However, as it is very difficult to culture
in the laboratory, information about its sensitivity to pollutants is lacking. We have successfully established a laboratory
clonal culture of Leptodora. In this study, acute toxicities of an insecticide and three heavy metals to Leptodora were estimated by using laboratory-cultured individuals. Our results suggest that Leptodora is more susceptible to contamination with those chemicals than the standard test organism, Daphnia. 相似文献
910.
Mari Kawaguchi Junya Hiroi Masaki Miya Mutsumi Nishida Ichiro Iuchi Shigeki Yasumasu 《BMC evolutionary biology》2010,10(1):260