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911.
Involvement of cathepsin B- and L-like proteinases in silk gland histolysis during metamorphosis of Bombyx mori 总被引:2,自引:0,他引:2
To identify proteinases involved in programmed cell death of the silk glands of Bombyx mori, we measured enzyme activities in silk gland homogenates. Several peptidyl-4-methylcoumaryl-7-amides (MCAs) and bovine hemoglobin were used as substrates in the presence and absence of proteinase inhibitors. The hydrolysis of t-butyloxycarbonyl-Phe-Ser-Arg-MCA (Boc-FSR-MCA), benzyloxy-carbonyl-Phe-Arg-MCA (Z-FR-MCA), and Z-Arg-Arg-MCA (Z-RR-MCA) was optimal at pH 5.5, 5.0, and 5.5, respectively. It was stimulated by the sulfhydryl compounds or EDTA and inhibited by both cysteine proteinase inhibitors and a cathepsin B-specific inhibitor, l-3-trans-(propyl-carbamoyl)oxirane-2-carbonyl)-L-isoleucyl-L-prolin (CA-074). The hemoglobin hydrolysis at the optimum pH 3.5 was inactivated by cysteine proteinase inhibitors, but stimulated slightly by pepstatin. The cleavage of Arg-MCA (R-MCA) and Leu-MCA (L-MCA) at optimum pH of 7.0 was strongly inhibited by an aminopeptidase inhibitor, puromycin, and by sulfhydryl compounds. The Boc-FSR-MCA, Z-FR-MCA, Z-RR-MCA, and hemoglobin hydrolyzing activities increased in the silk glands dramatically after cocoon formation, while the R-MCA and L-MCA cleaving activities declined. The results strongly suggest the involvement of cathepsin B- and cathepsin L-like proteinases in the histolysis of the silk gland during metamorphosis. 相似文献
912.
913.
Osawa Y Nagaki M Banno Y Brenner DA Nozawa Y Moriwaki H Nakashima S 《Journal of immunology (Baltimore, Md. : 1950)》2003,170(8):4053-4060
914.
915.
916.
917.
Sequences of two internal transcribed spacer regions between 18S and 28S rRNA for recently described yeasts species, Saccharomyces kunashirensis, S. martiniae, S. rosinii, and S. transvaalensis, were determined to assess their phylogenetic relationship to the other Saccharomyces species. In the two phylogenetic trees constructed by the neighbor-joining method, independent branches reflected that delimitation
of the four new species was valid.
Received: 17 June 1998 / Accepted: 7 August 1998 相似文献
918.
I. Löw W. Jahn Th. Wieland S. Sekita K. Yoshihira S. Natori 《Analytical biochemistry》1979,95(1):14-18
The mold metabolites chaetoglobosins Ch-A, B, C, E, F, and J exert, as do the cytochalasins CB, CD, CE, and CG, enhancing effects of various strength on the polymerization of rabbit muscle G-actin. The polymers formed differ widely in their viscosity, Ch-B and Ch-J leading to the least viscous actins. Equal states of viscosity are arrived at by interaction of F-actin with the respective drugs. There is no correlation between the ATP hydrolyzing activity of F-actin elicited by the various cytochalasins and their influence on the viscosity. 相似文献
919.
920.
Hiroyuki Tomita Kaori Tanaka Akihiro Hirata Hideshi Okada Hisashi Imai Yohei Shirakami Kotaro Ohnishi Shigeyuki Sugie Hitomi Aoki Yuichiro Hatano Kei Noguchi Tomohiro Kanayama Ayumi Niwa Natsuko Suzui Tatsuhiko Miyazaki Takuji Tanaka Haruhiko Akiyama Masahito Shimizu Akira Hara 《Cell reports》2021,34(8):108772
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