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451.
H. Malekinejad A. Tukmechi H. Ebrahimi B. Bazargani-Gilani 《World journal of microbiology & biotechnology》2011,27(1):147-151
Recently a new method of antibacterial susceptibility testing has been developed, which based on the reduction of the 3-(4,
5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) by bacteria. This study aimed to improve the latest developed
method in terms of the period of time, concentration of bacterium, accuracy of assay and minimizing the toxic effect of the
MTT solution. The commonly used broth microdilution method was compared in this study, as well. The minimum inhibition concentration
(MIC) of the enrofloxacine against Aeromonas hydrophila as an opportunistic and ubiquitous bacterium was tested by using the improved MTT method. Using both methods showed that
enrofloxacine exerts an excellent antibacterial effect against A. hidrophyla with MIC value of 62.5 ng/ml. The simplicity of the improved MTT method was shown with performing all steps of the assay
in one plate, using rather low concentration of the bacterium (500 CFU/well) and shortening of the incubation time to 9 h. Moreover, 5–30 min incubation of bacteria with MTT solution excludes
any toxic effect of tetrazolium salt against bacteria. Thus, our results suggest that enrofloxacine might be considered as
a potent antibacterial agent against A. hydrophila induced contaminations. Moreover, MTT method with current improved characteristics such as short incubation time, low concentration
of bacteria, and high sensitivity could be more practical alternative for the broth microdilution method in antibacterial
susceptibility testing protocol. 相似文献
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Ebrahimi Elham Shabestari Rima Manafi Bashash Davood Safa Majid 《Molecular biology reports》2022,49(3):2025-2036
Molecular Biology Reports - Myeloid cell leukemia-1 (MCL-1) is a component of the Bcl-2 anti-apoptotic family that plays a key role in cell proliferation and differentiation. Despite tremendous... 相似文献
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Abdolalian Mehrnaz Ebrahimi Maryam Aghamirzadeh Mahansadat Eshraghi Nooshin Moghaddasi Mana Eslamnik Parvinsadat 《Journal of molecular histology》2021,52(4):635-642
Journal of Molecular Histology - Endothelial dysfunction is considered as the main hallmark of Preeclampsia (PE). Despite the unknown pathogenesis of PE, different possible causes have been... 相似文献
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Rouhollah Amini Atefeh Ebrahimi Adel Dabbagh Mohammadi Nasab 《Plant Species Biology》2021,36(2):338-347
Astrodaucus orientalis is a weed species in cropping systems and rangelands in Iran. The effects of temperature, light, NaCl concentration, water potential, seed burial depth and crop residue cover were assessed on seed germination and seedling emergence of two populations of A. orientalis from Ardabil (Meshginshahr population) and East Azarbayjan (Tabriz population) provinces of Iran. The A. orientalis populations indicated different responses to environmental factors and burial depth. In the Tabriz population the greatest germination (88.5%) was observed in 20/12°C day/night temperature but in the Meshginshahr population (83.2%) it was obtained in 24/16°C day/night temperature. Over a broad range of light period (10–24 hr light) germination was 74–83%, but it decreased (less than 37%) under 24 hr dark in both A. orientalis populations. With respect to water potential, the C50 parameters were −0.62 and − 0.49 MPa for Tabriz and Meshginshahr populations, respectively. The D50 parameters (the burial depth that caused 50% decrease in emergence) for Tabriz and Meshginshahr populations were 2.42 and 3.13 cm, respectively. Generally, the results showed that emergence of both populations of A. orientalis was delayed as depth of burial increased up to 4 cm and in cropping systems a shallow tillage that locates the seeds to >4 cm of depth in soil could be used in order to suppress seedling emergence. Our findings also could be useful in integrated management of A. orientalis in winter annual crops and rangelands. 相似文献
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