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21.
Hisato Saitoh Maryann Delli Pizzi Jian Wang 《The Journal of biological chemistry》2002,277(7):4755-4763
Ubc9, a conjugation enzyme for the ubiquitin-related modifier SUMO, is present predominantly in the nucleus and at the nuclear pore complex. The functional significance of its subcellular compartmentalization, however, remains to be elucidated. Here, we define a Pro-Glu-Asp-Ser-Thr-rich element containing 129 amino acid residues, designated IR1+2, on the human nucleoporin RanBP2/Nup358, which binds directly to Ubc9 with high affinity both in vitro and in vivo. When IR1+2 tagged with green fluorescence protein at its amino terminus (GFP-IR1+2) was transfected into COS-7 cells, we found that approximately 90% of the nuclear Ubc9 was sequestered in the cytoplasm. We also observed that both SUMO-1 and SUMO-2/3 were mislocalized, and promyelocytic leukemia protein PML formed an enlarged aggregate in the nucleus. Moreover, the homologous recombination protein Rad51 mislocalized to the cytoplasm, and Rad51 foci, a hallmark of functional association of Rad51 with damaged DNA, did not form efficiently even in the presence of a DNA strand breaker. These findings emphasize that the IR1+2 domain is a useful tool for manipulating the nuclear localization of Ubc9 and perturbing the subcellular localization of SUMOs and/or SUMOlated proteins, and they emphasize the important role of nuclear Ubc9 in the Rad51-mediated homologous recombination pathway, possibly by modulating intracellular trafficking of Rad51. 相似文献
22.
The activity of the yeast activator HAP1in vivo requires heme. A heme responsive domain of HAP1 was identified previously. It is adjacent to the DNA-binding domain, and appears to block DNA binding in the absence of heme. Here we describe a novel genetic selection which yielded mutants of HAP1 that are independent of heme when assayed for activation. These mutants define a second region of HAP1, close to the activation domain, which also controls its response to heme. 相似文献
23.
Erin Quartley Andrei Alexandrov Maryann Mikucki Frederick S. Buckner Wim G. Hol George T. DeTitta Eric M. Phizicky Elizabeth J. Grayhack 《Journal of structural and functional genomics》2009,10(3):233-247
High level expression of many eukaryotic proteins for structural analysis is likely to require a eukaryotic host since many
proteins are either insoluble or lack essential post-translational modifications when expressed in E. coli. The well-studied eukaryote Saccharomyces cerevisiae possesses several attributes of a good expression host: it is simple and inexpensive to culture, has proven genetic tractability,
and has excellent recombinant DNA tools. We demonstrate here that this yeast exhibits three additional characteristics that
are desirable in a eukaryotic expression host. First, expression in yeast significantly improves the solubility of proteins
that are expressed but insoluble in E. coli. The expression and solubility of 83 Leishmania major ORFs were compared in S. cerevisiae and in E. coli, with the result that 42 of the 64 ORFs with good expression and poor solubility in E. coli are highly soluble in S. cerevisiae. Second, the yield and purity of heterologous proteins expressed in yeast is sufficient for structural analysis, as demonstrated
with both small scale purifications of 21 highly expressed proteins and large scale purifications of 2 proteins, which yield
highly homogeneous preparations. Third, protein expression can be improved by altering codon usage, based on the observation
that a codon-optimized construct of one ORF yields three-fold more protein. Thus, these results provide direct verification
that high level expression and purification of heterologous proteins in S. cerevisiae is feasible and likely to improve expression of proteins whose solubility in E. coli is poor. 相似文献
24.
Christopher Looze David Yui Lester Leung Matthew Ingham Maryann Kaler Xianglan Yao Wells W. Wu Rong-Fong Shen Mathew P. Daniels Stewart J. Levine 《Biochemical and biophysical research communications》2009,378(3):433-438
Exosomes are nanovesicles that are released from cells as a mechanism of cell-free intercellular communication. Only a limited number of proteins have been identified from the plasma exosome proteome. Here, we developed a multi-step fractionation scheme incorporating gel exclusion chromatography, rate zonal centrifugation through continuous sucrose gradients, and high-speed centrifugation to purify exosomes from human plasma. Exosome-associated proteins were separated by SDS–PAGE and 66 proteins were identified by LC–MS/MS, which included both cellular and extracellular proteins. Furthermore, we identified and characterized peroxisome proliferator-activated receptor-γ (PPARγ), a nuclear receptor that regulates adipocyte differentiation and proliferation, as well as immune and inflammatory cell functions, as a novel component of plasma-derived exosomes. Given the important role of exosomes as intercellular messengers, the discovery of PPARγ as a component of human plasma exosomes identifies a potential new pathway for the paracrine transfer of nuclear receptors. 相似文献
25.
Alessandra Corradetti Franca Saccucci Monica Emanuelli Giorgia Vagnoni Monia Cecati Davide Sartini Stefano R. Giannubilo Andrea L. Tranquilli 《Cell stress & chaperones》2010,15(1):95-100
Mitogen-activated protein kinase (MAPK) p38α was shown to be implicated in the organogenesis of the placenta, and such placental
alteration is crucial for the development of hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome. We aimed
to analyze for the first time human placental expression of MAPK p38α in pregnancies complicated by HELLP. The placental expression
of MAPK p38α was investigated by semiquantitative polymerase chain reaction using cDNA extracted from placental tissue of
15 pregnancies with HELLP syndrome and 15 gestational age-matched controls. Seven patients with HELLP also had intrauterine
fetal growth restriction (IUGR). In placenta from pregnancy complicated by HELLP, the expression of MAPK p38α is significantly
decreased compared to the group with normal pregnancy (p < 0.001), while no difference was found between the HELLP and HELLP with IUGR subpopulations. Our study shows for the first
time that MAPK p38α is expressed in the human placenta. Pregnancies with placental dysfunction and hypertensive complications
are characterized by a significantly decreased expression of MAPK p38α. Our observations suggest that p38 MAPK signaling may
be essential in placental angiogenesis and functioning. 相似文献
26.
27.
Comparative RFLP mapping of Triticum monococcum genes controlling vernalization requirement 总被引:3,自引:0,他引:3
J. Dubcovsky D. Lijavetzky L. Appendino G. Tranquilli 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):968-975
The adaptability of Triticum aestivum to a large range of environments is partially due to genetic differences in sensitivity to vernalization. The most potent
gene reducing the vernalization requirement in hexaploid wheat is Vrn-A1. An orthologous vernalization gene, designated Vrn-A
m
1, was mapped in the diploid wheat Triticum monococcum between RFLP markers Xwg908 and Xabg702 on the long arm of chromosome 5AmL. The orthology of VrnA
m
1 with Vrn-A1 (5A wheat, originally Vrn1), Vrn-D1 (5D wheat, originally Vrn3), Vrn-R1 (5R rye, originally Sp1) and Vrn-H1 (5H barley, originally Sh2) was shown by mapping RFLP markers linked to these vernalization genes on the T. monococcum linkage map. A second vernalization gene, designated Vrn-A
m
2, was found in the distal region of chromosome 5AmL within a segment translocated from homoeologous group 4. This gene is completely linked to RFLP marker Xbcd402 and located between the same RFLP markers (Xβ-Amy-1 and Xmwg616) as the Vrn-H2 (originally Sh) locus in Hordeum vulgare.
Received: 6 January 1998 / Accepted: 31 March 1998 相似文献
28.
Javier I. Escobar Eugenia T. Randolph Maryann Hill 《Culture, medicine and psychiatry》1986,10(3):259-276
This study explores cultural influences on symptoms and course of schizophrenic disorder. Hispanic veterans with schizophrenia are compared to a similar group of White non-Hispanic (Anglo) veterans. Symptoms were elicited with a structured diagnostic interview and a battery of rating instruments. Primary symptoms of schizophrenia (e.g., hallucinations, delusions, functional deterioration) were very similar for both groups. However, Hispanic patients reported a later age of onset, exhibited more somatization, and spent less time in the hospital than Anglo patients.This study was supported by a grant from the Veterans Administration Health Services Research and Development Service (Project 11R 81-633). 相似文献
29.
Replication and Complementation of Human Adenoviruses and Simian Papovavirus at an Elevated Temperature 总被引:3,自引:2,他引:1
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The simian papovavirus SV40 replicated as well in simian cells incubated at 41 C as in cells incubated at 37 C, although the latent period was shortened at the elevated temperature. Human adenoviruses differed in their responses to the elevated temperature. Some serotypes, such as 3, 4, 5, 7, 8, 16, and 21, replicated as well, or almost as efficiently, in human cells incubated at 41 C as in cells incubated at 37 C, whereas with other serotypes, such as 1, 2, 6, 12, and 14, maximal yields in cultures incubated at 41 C were much lower than the yields from companion cultures incubated at 37 C. This difference was also detected in simian cells co-infected with SV40 and a human adenovirus; maximal complementation occurred with some serotypes at the elevated temperature but not with other serotypes. The degree of complementation observed in the simian cells at 41 C was directly correlated with the ability of the adenovirus to replicate at 41 C in human cells. Therefore, the capacity of SV40 to serve as a helper virus is not affected by the elevated temperature, showing that the complementation event supplied by the simian virus is heat-stable between 37 and 41 C. Maximal complementation appeared to depend upon a characteristic present in the adenovirus genome. 相似文献
30.
Yao X Dai C Fredriksson K Lam J Gao M Keeran KJ Nugent GZ Qu X Yu ZX Jeffries N Lin J Kaler M Shamburek R Costello R Csako G Dahl M Nordestgaard BG Remaley AT Levine SJ 《American journal of physiology. Lung cellular and molecular physiology》2012,302(2):L206-L215
Apolipoprotein E (apoE) is an endogenous negative regulator of airway hyperreactivity (AHR) and mucous cell metaplasia in experimental models of house dust mite (HDM)-induced airway disease. The gene encoding human apoE is polymorphic, with three common alleles (ε2, ε3, and ε4) reflecting single amino acid substitutions at amino acids 112 and 158. The objective of this study was to assess whether the human apoE alleles modify airway responses to repeated nasal HDM challenges. Mice expressing the human apoE ε2 (huApoE2), ε3 (huApoE3), or ε4 (huApoE4) alleles received nasal HDM challenges, and airway responses were compared with mice expressing the endogenous murine apoE gene (muApoE). huApoE3 mice displayed significant reductions in AHR, mucous cell metaplasia, and airway inflammation compared with muApoE mice. The attenuated severity of airway inflammation in huApoE3 mice was associated with reductions in lung mRNA levels of Th2 and Th17 cytokines, as well as chemokines (CCL7, CCL11, CCL24). huApoE4 mice had an intermediate phenotype, with attenuated AHR and IgE production, compared with muApoE mice, whereas airway inflammation and mucous cell metaplasia were not reduced. In contrast, HDM-induced airway responses were not modified in mice expressing the huApoE2 allele. We conclude that the polymorphic huApoE alleles differentially modulate HDM-induced airway disease, which can be stratified, in rank order of increasing disease severity, ε3 < ε4 < ε2. These results raise the possibility that the polymorphic apoE alleles may modify disease severity in human asthma. 相似文献