全文获取类型
收费全文 | 1371篇 |
免费 | 91篇 |
国内免费 | 4篇 |
出版年
2024年 | 4篇 |
2023年 | 27篇 |
2022年 | 60篇 |
2021年 | 97篇 |
2020年 | 91篇 |
2019年 | 166篇 |
2018年 | 98篇 |
2017年 | 78篇 |
2016年 | 82篇 |
2015年 | 72篇 |
2014年 | 87篇 |
2013年 | 123篇 |
2012年 | 108篇 |
2011年 | 88篇 |
2010年 | 57篇 |
2009年 | 50篇 |
2008年 | 45篇 |
2007年 | 18篇 |
2006年 | 26篇 |
2005年 | 20篇 |
2004年 | 20篇 |
2003年 | 19篇 |
2002年 | 16篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1988年 | 2篇 |
1985年 | 1篇 |
排序方式: 共有1466条查询结果,搜索用时 15 毫秒
191.
Maryam Amiri Mohsen Basiri Hossein Eskandary Zeinab Akbarnejad Marzie Esmaeeli Yaser Masoumi-Ardakani 《Electromagnetic biology and medicine》2018,37(3):138-145
Glioblastoma multiforme (GBM) is a malignant brain cancer that causes high mortality in patients. GBM responds weakly to the common cancer treatments such as chemotherapy and radiotherapy and even surgery. Carboplatin is an alkylating agent widely used to treat cancer. However, resistance to this drug is a common problem in its use in cancer treatment. Concomitant exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and carboplatin is one unexplored possibility for overcoming this resistance. Indeed, many lines of evidence show that EMF affects cancer cells and drug action. In this study, we evaluated the effect of concomitant administration of carboplatin and EMF (50 Hz, 70 G) and also concomitant administration of carboplatin and static magnetic field (SMF) (70 G) on human glioma cell line (U-87). The results showed that cotreatment reduced the efficiency of carboplatin in U-87 cells, by decreasing caspase-3 in comparison to drug groups. Overall, EMF reduced the apoptotic effect of carboplatin, possibly through a redox regulation mechanism. Therefore, we have to avoid coadministration of magnetic field (MF) and carboplatin in tumor area, because the MF decreased the toxicity of the drug. However, further studies are needed to reveal the action mechanism of this combination therapeutic method. 相似文献
192.
Mahdavi Manijeh Koulivand Leila Khorrami Mehdi Mirsafaie Maryam Kheirollahi Majid 《Molecular biology reports》2018,45(5):1165-1173
Molecular Biology Reports - Cystinuria is an autosomal recessive defect in reabsorptive transport of cystine and the dibasic amino acids ornithine, arginine, and lysine from renal tubule and small... 相似文献
193.
Nobakht-Haghighi Navid Rahimifard Mahban Baeeri Maryam Rezvanfar Mohammad Amin Moini Nodeh Shermineh Haghi-Aminjan Hamed Hamurtekin Emre Abdollahi Mohammad 《Molecular and cellular biochemistry》2018,449(1-2):267-276
Molecular and Cellular Biochemistry - Oxidative stress has been involved in the aging process and the pathogenesis of type-2 diabetes, which is a serious health problem worldwide. This study... 相似文献
194.
195.
Mona Navaei-Nigjeh Milad Moloudizargari Maryam Baeeri Mahdi Gholami Nasrin Lotfibakhshaiesh Masoud Soleimani Ebrahim Vasheghani-farahani Jafar AI Mohammad Abdollahi 《Cytotherapy》2018,20(9):1124-1142
Background aims
Adipose tissue–derived mesenchymal stromal cells (AT-MSCs), widely known as multipotent progenitors, release several cytokines that support cell survival and repair. There are in vitro and in vivo studies reporting the regenerative role of AT-MSCs possibly mediated by their protective effects on functional islet cells as well as their capacity to differentiate into insulin-producing cells (IPCs).Methods
On such a basis, our goal in the present study was to use three different models including direct and indirect co-cultures and islet-derived conditioned medium (CM) to differentiate AT-MSCs into IPCs and to illuminate the molecular mechanisms of the beneficial impact of AT-MSCs on pancreatic islet functionality. Furthermore, we combined in vitro co-culture of islets and AT-MSCs with in vivo assessment of islet graft function to assess whether co-transplantation of islets with AT-MSCs can reduce marginal mass required for successful islet transplantation and prolong graft function in a diabetic rat model.Results
Our findings demonstrated that AT-MSCs are suitable for creating a microenvironment favorable for the repair and longevity of the pancreas β cells through the improvement of islet survival and maintenance of cell morphology and insulin secretion due to their potent properties in differentiation. Most importantly, hybrid transplantation of islets with AT-MSCs significantly promoted survival, engraftment and insulin-producing function of the graft and reduced the islet mass required for reversal of diabetes.Conclusions
This strategy might be of therapeutic potential solving the problem of donor islet material loss that currently limits the application of allogeneic islet transplantation as a more widespread therapy for type 1 diabetes. 相似文献196.
Maryam Gazor Seyed Siamak Ashraf Talesh Alireza kavianpour Maryam Khatami Amin Javidanbardan Seyed Nezamedin Hosseini 《Biotechnology and Bioprocess Engineering》2018,23(1):49-54
In biotechnological processes, often cell disruption has been an inevitable step as current host cells express most of the desired products intracellularly. Thus, an appropriate cell disruption technique must be selected considering different factors including the target product, process scale, and cell wall structure. In the current study, as a novel method, the efficacy of cell disruption via laser was tested qualitatively and quantitatively in batch and continuous systems, respectively. Laser-induced cell lysis can be a clean, rapid and convenient alternative to the other conventional disruption techniques. Our investigations in the continuous system with a flow rate of 800 μL/sec proved efficient (~ 90%) Pichia pastoris cell disruption at the wavenumber 1,064 nm with the energy input of 284 mW after four complete rounds of circulation. The main mechanism of cell disruption is assumed to be thermolysis via instant heat increase in the laser-treated spot. The results of the current study showed that continuous laser system could be applied in laboratory and industry scale for cell disruption. 相似文献
197.
Improvement of both lipid and biomass productivities of Qatar Chlorocystis isolate for biodiesel production and food security 下载免费PDF全文
Imen Saadaoui Touria Bounnit Maryam Muraikhi Rihab Rasheed Ghamza Alghasal Hareb Al Jabri 《Phycological Research》2018,66(3):182-188
Microalgae are considered a very promising alternative for biofuel production. Several strategies were developed to modulate and improve algae metabolites production to meet the requirements for biodiesel production. Most previous research evidenced that the increase of the lipid content is accompanied by a decrease of the biomass production, which increases the cost of the downstream processing. Hence, the challenge is to find special culture conditions that increase the lipid and the biomass productivities simultaneously. In the present work, we developed a strategy for the improvement of biomass and lipid productivities in a novel local microalga isolate, Chlorocystis sp. QUCCCM14, which was not previously known as a promising strain. Indeed, culturing QUCCCM14 using f/2 medium with 10× NaH2PO4 (0.15 g L?1 NaNO3 and 5.6 mg L?1 NaH2PO4) resulted in an improvement of 3.178 folds the lipid productivity reaching 56.121 mg L?1 day?1 and enhanced the biomass productivity reaching 141.363 mg L?1 day?1, simultaneously. Comparative analyses of the FAME profiles demonstrated that fed‐batch culture with phosphate or nitrate separately leads to a high production of the omega 3 fatty acids (Linolenic acid), whereas fed‐batch culture with phosphate and nitrate simultaneously increased the production of fatty acids suitable for biodiesel production. 相似文献
198.
The understanding of host specificity of predacious insects is one of the most important problems in the investigation of biological control. Prey preference and the potential for prey switching in Anthocoris minki Dohrn (Hem.: Anthocoridae) was assessed in two- choice experiments under environmentally controlled conditions. Manly's preference index was used to compare A. minki predation on Agonoscena pistaciae Burckhardt and Lauterer (Hem.: Psyllidae) versus Psyllopsis repens Loginova (Hem.: Psyllidae) but showed no statistically significant evidence for preference. Anthocoris minki did prefer psyllids (Agonoscena pistaciae and Psyllopsis repens) over aphids (Forda hirsuta Mordvilko (Hem.: Pemphigidae) and Aphis craccivora Koch (Hem.: Aphididae)). The predator also consumed significantly more 2nd instar than 4th instar nymphs of P. repens. Switching behavior experiments showed that A. minki did not preferentially attack the most abundant prey when the prey combination was either biased toward A. pistaciae or toward F. hirsuta. Linear regression between preference indices and prey ratios indicated an overall preference for psyllids over aphids. The results suggest that a psyllid-specialized feeder such as A. minki is more likely to be effective biocontrol agent against psyllid species and less effective at reducing the numbers of other pest species that might co-occur with psyllids. 相似文献
199.
Maryam Ahmadian Sihao Liu Shannon M. Reilly Nasun Hah Weiwei Fan Eiji Yoshihara Pooja Jha C. Daniel De Magalhaes Filho Sandra Jacinto Andrew V. Gomez Yang Dai Ruth T. Yu Christopher Liddle Annette R. Atkins Johan Auwerx Alan R. Saltiel Michael Downes Ronald M. Evans 《Cell reports》2018,22(11):2849-2859
200.